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22238 Amylase, Other

Amylase, Other
Test Code: AMY-O
Synonyms/Keywords
Body Fluid Amylase​
Useful For
Aids in distinguishing between pseudocysts and other types of pancreatic cysts when used in conjunction with imaging studies cytology and other pancreatic cyst tumor markers.
If ascites are present, it is occasionally used to demonstrate pancreatic inflammation. If this is true, the level will be at least ten times that of serum.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Pancreatic Cyst Fluid / Body Fluid​
Body Fluid container​
 ​Plastic vial 300 uL 100 uL ​N/A
Collection Processing Instructions
Do not collect the specimen with preservatives or anticoagulants.
Specimens should be centrifuged to remove cells or clots if present.
Specimen Stability Information
Specimen Type Temperature Time
Pancreatic Cyst Fluid / Body Fluid​ ​ ​ ​ Ambient N/A
​Refrigerated ​7 days
​Frozen at -20 deg Celsius ​30 days
​Frozen at -70 deg Celsius ​> 30 days
Rejection Criteria
Blood or urine
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Sunday​ Less than 2 hours​ CNPG3 Rate Assay/Beckman AU
Reference Range Information
Performing Location

Reference Range

Marshfield​

Reference cut-off limits for Amylase for pancreatic cyst fluid and other body fluids have not been established or verified.

The test results should not be the sole basis of diagnosis and always be correlated with imaging and cytology.

Interpretation
Measurement of amylase in pancreatic cyst fluids is often used in conjunction with tumor markers, carcinoembryonic antigen (CEA) and CA 19-9 as a differential diagnosis of pancreatic cyst lesions. Low levels of amylase are observed to be useful in excluding pancreatic pseudocysts.

Based on evidence available and recommendations by the American College of Gastroenterology (ACG) practice guidelines for the Diagnosis and Management of Neoplastic Pancreatic Cysts (Am. J. Gastroenterol. 2007; 102: 2339-2349), a pancreatic cyst fluid amylase concentration < 250 U/L has high specificity to excludes pseudocysts and more consistent with cystic neoplasms (mucinous cystic neoplasm, intra-ductal papillary mucinous neoplasm, serous cystadenomas, cystic neuroendocrine tumor and mucinous cystadenocarcinomas).
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
82150   ​ ​ ​ ​ ​
Synonyms/Keywords
Body Fluid Amylase​
Ordering Applications
Ordering Application Description
​Centricity ​Amylase
​Cerner ​Amylase Level Body Fluid
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Pancreatic Cyst Fluid / Body Fluid​
Body Fluid container​
 ​Plastic vial 300 uL 100 uL ​N/A
Collection Processing
Do not collect the specimen with preservatives or anticoagulants.
Specimens should be centrifuged to remove cells or clots if present.
Specimen Stability Information
Specimen Type Temperature Time
Pancreatic Cyst Fluid / Body Fluid​ ​ ​ ​ Ambient N/A
​Refrigerated ​7 days
​Frozen at -20 deg Celsius ​30 days
​Frozen at -70 deg Celsius ​> 30 days
Rejection Criteria
Blood or urine
Useful For
Aids in distinguishing between pseudocysts and other types of pancreatic cysts when used in conjunction with imaging studies cytology and other pancreatic cyst tumor markers.
If ascites are present, it is occasionally used to demonstrate pancreatic inflammation. If this is true, the level will be at least ten times that of serum.
Reference Range Information
Performing Location

Reference Range

Marshfield​

Reference cut-off limits for Amylase for pancreatic cyst fluid and other body fluids have not been established or verified.

The test results should not be the sole basis of diagnosis and always be correlated with imaging and cytology.

Interpretation
Measurement of amylase in pancreatic cyst fluids is often used in conjunction with tumor markers, carcinoembryonic antigen (CEA) and CA 19-9 as a differential diagnosis of pancreatic cyst lesions. Low levels of amylase are observed to be useful in excluding pancreatic pseudocysts.

Based on evidence available and recommendations by the American College of Gastroenterology (ACG) practice guidelines for the Diagnosis and Management of Neoplastic Pancreatic Cysts (Am. J. Gastroenterol. 2007; 102: 2339-2349), a pancreatic cyst fluid amylase concentration < 250 U/L has high specificity to excludes pseudocysts and more consistent with cystic neoplasms (mucinous cystic neoplasm, intra-ductal papillary mucinous neoplasm, serous cystadenomas, cystic neuroendocrine tumor and mucinous cystadenocarcinomas).
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Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Sunday​ Less than 2 hours​ CNPG3 Rate Assay/Beckman AU
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
82150   ​ ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.