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22299 Antibody Identification, RBC Antibodies

Antibody Identification, RBC Antibodies
Test Code: ABSC
Synonyms/Keywords
​Atypical Antibody Panel, Antibody ID, Red Cell Antibody, ABID
Test Components
Antibody identification is not a provider orderable test; FOR INTERNAL USE ONLY.
Laboratories requesting antibody identification should order ABSC. 
 
Testing may include the following components: ABSC, ABID, DAT 
Useful For
​Identifying unexpected red cell antibodies when the antibody screen is positive.  This is important to provide compatible, antigen negative red cell products for transfusion.
 
Identifying antibody(ies) in prenatal patients to assist in determining the risk for Hemolytic Disease of the Fetus and Newborn (HDFN).
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Whole blood​
6 mL EDTA
Pink Top Tube
(PTT)​
Two 3 mL EDTA Lavender Top Tubes (LTT)
OR  
6 mL Red Top Tube (RTT)​
 6 mL ​ 2.5 mL ​ Four  0.5 mL microcollection tubes​
Collection Processing Instructions
​Invert specimen at least 5-6 times in order to facilitate mixing and prevent clotting.  Do not separate plasma from red cells.
Specimen Stability Information
Specimen Type Temperature Time
Whole blood/serum​ Refrigerate​ 4 days old​
Rejection Criteria
Serum separator tubes (SST)
Frozen​
Hemolyzed​
Interference
​Fresh serum, fibrin or particulate matter in the patient specimen may cause anomalous results.  EDTA plasma may minimize these interferences.
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Monday through Sunday​ typically 4 hours*
Solid Phase Red Cell Adherence
PEG enhanced ID panel
​*Based on complexity of antibodies present, may take up to 24 hours. ​ ​ ​
Test Information
​For other laboratories solely requesting Antibody Identification for positive Antibody Screen results:
Contact Transfusion Service with current test results and patient information at 800-222-5835 ext. 16262 before sending specimen. Complete a Request for Additional Serologic Testing at Marshfield Center
form to Include patient diagnosis, history of transfusions, pregnancies and administration of Rh(D) Immune Globulin, if applicable.  Send copies of serological work and include a copy of screen cell antigram.
 
Other Testing Performed: Tests may or may not be performed, at additional charge depending on the results and interpretation of the initial tests.  Refer to CPT tables for additional testing codes and description.
 
The following common antibodies (not inclusive) are considered clinically significant and have been implicated in Hemolytic Disease of the Fetus and Newborn and/or hemolytic transfusion reactions: Anti-D, Anti-E, Anti-C, Anti-e, Anti-c, Anti-K, Anti-k, Anti-Fya, Anti-Fyb, Anti-Jka, Anti-Jkb, Anti-S, Anti-s, and IgG form of Anti-M. Antigen regative red cells should be selected for transfusion when antibodies are detected or patient has a history of the antibody.
Reference Range Information
Performing Location Reference Range
​Marshfield No reference ranges are available.  ​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​86870 ​ ​ ​ABID (additional antibody panels) ​
​86077 ​ PATH CONSULT ​
86850​ ​ ​ ​ABSC ​
​86880 ​ ​ ​DAT ​
​86905 ​ ​ ​RBC antigen (patient or donor unit) ​
86886 ​ ​ ​Antibody titer ​
​86860 ​ ​ ​Elution ​
​86978 ​ ​ ​Cold adsorption* ​
​86978 ​ ​ ​Warm adsorption* ​
​86971 ​ ​ ​Ficin treatment ​
​86977 ​ ​ ​Neutralization or acidification ​
86920
86921
86922
 ​ ​ ​IAT crossmatch ​
​*charge will be for quantity of adsorptions performed ​ ​ ​ ​
Synonyms/Keywords
​Atypical Antibody Panel, Antibody ID, Red Cell Antibody, ABID
Test Components
Antibody identification is not a provider orderable test; FOR INTERNAL USE ONLY.
Laboratories requesting antibody identification should order ABSC. 
 
Testing may include the following components: ABSC, ABID, DAT 
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Whole blood​
6 mL EDTA
Pink Top Tube
(PTT)​
Two 3 mL EDTA Lavender Top Tubes (LTT)
OR  
6 mL Red Top Tube (RTT)​
 6 mL ​ 2.5 mL ​ Four  0.5 mL microcollection tubes​
Collection Processing
​Invert specimen at least 5-6 times in order to facilitate mixing and prevent clotting.  Do not separate plasma from red cells.
Specimen Stability Information
Specimen Type Temperature Time
Whole blood/serum​ Refrigerate​ 4 days old​
Rejection Criteria
Serum separator tubes (SST)
Frozen​
Hemolyzed​
Interference
​Fresh serum, fibrin or particulate matter in the patient specimen may cause anomalous results.  EDTA plasma may minimize these interferences.
Useful For
​Identifying unexpected red cell antibodies when the antibody screen is positive.  This is important to provide compatible, antigen negative red cell products for transfusion.
 
Identifying antibody(ies) in prenatal patients to assist in determining the risk for Hemolytic Disease of the Fetus and Newborn (HDFN).
Test Components
Antibody identification is not a provider orderable test; FOR INTERNAL USE ONLY.
Laboratories requesting antibody identification should order ABSC. 
 
Testing may include the following components: ABSC, ABID, DAT 
Reference Range Information
Performing Location Reference Range
​Marshfield No reference ranges are available.  ​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Monday through Sunday​ typically 4 hours*
Solid Phase Red Cell Adherence
PEG enhanced ID panel
​*Based on complexity of antibodies present, may take up to 24 hours. ​ ​ ​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​86870 ​ ​ ​ABID (additional antibody panels) ​
​86077 ​ PATH CONSULT ​
86850​ ​ ​ ​ABSC ​
​86880 ​ ​ ​DAT ​
​86905 ​ ​ ​RBC antigen (patient or donor unit) ​
86886 ​ ​ ​Antibody titer ​
​86860 ​ ​ ​Elution ​
​86978 ​ ​ ​Cold adsorption* ​
​86978 ​ ​ ​Warm adsorption* ​
​86971 ​ ​ ​Ficin treatment ​
​86977 ​ ​ ​Neutralization or acidification ​
86920
86921
86922
 ​ ​ ​IAT crossmatch ​
​*charge will be for quantity of adsorptions performed ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.