Aspartate Amino Transferase

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Test Code: AST

Overview
Ordering
Specimen
Performing
Clinical/Interpretive
Contacts
Coding

Synonyms/Keywords

SGOT, AST, GOT, Aspartrate Transaminase

Useful For

AST is used primarily in the diagnosis of liver and heart muscle disease. AST is also elevated in 97% of myocardial infarction patients.

Specimen Requirements

Fasting Required	Specimen Type	Preferred Container/Tube	Acceptable Container/Tube	Specimen Volume	Specimen Minimum Volume (allows for 1 repeat)	Pediatric Minimum Volume (no repeat)
No	Plasma/Serum	Lithium-heparin Plasma Separator (PST), Serum Separator Tube (SST)	Lithium or Sodium-heparin Green Top (GTT), Red Top Tube (RTT)	1 mL	0.3 mL	0.7 mL whole blood

Collection Processing Instructions

Samples collected in a RTT or GGT must be removed from cells within one hour for storage or transport. Samples collected in gel barrier tubes must be removed from the primary tube prior to transporting to Marshfield. Do not send the primary collection tube.

Specimen Stability Information

Specimen Type	Temperature	Time
Plasma/Serum	Ambient	5 days
	Refrigerate	7 days
	Frozen	>7 days to 3 months

Rejection Criteria

Grossly hemolyzed samples
Samples collected with sodium citrate, potassium oxalate, or sodium fluoride preservatives

Interference

Hemolysis will falsey increase AST result.

Sulfasalazine may interfere with Dimension AST assays (Regional labs) and cause falsely depressed results.

For patients receiving treatment, venipuncture should occur prior to drug administration to prevent this interference.

Performing Laboratory Information

Performing Location	Day(s) Test Performed	Analytical Time	Methodology/Instrumentation
Chippewa Falls	Monday through Friday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Diagnostic Treatment Center	Monday through Sunday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Eau Claire	Monday through Sunday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Flambeau Hospital	Monday through Sunday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Ladysmith Medical Center	Monday through Sunday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Lake Hallie	Monday through Sunday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Lakeview Medical Center	Monday through Sunday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Marshfield	Monday through Sunday	Less than 2 hours	(IFCC), UV/NADH Rate Assay/Beckman AU
Merrill	Monday through Saturday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Minocqua	Monday through Sunday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Neillsville	Monday through Sunday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Stevens Point	Monday through Friday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Wausau	Monday through Sunday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Wisconsin Rapids	Monday through Friday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension

Reference Range Information

Performing Location	Reference Range
All Performing Sites using Dimension	0 - 7 days: 20-96 IU/L 8 days - 3 years: 16-58 IU/L 4 years - 6 years: 10-47 IU/L Male: 7 - 15 years: 10-36 IU/L Male: >=16 years: 15-46 IU/L Female: 7 - 19 years: 10-36 IU/L Female: >= 20 years: 14-41 IU/L
Marshfield	Both: 0 - 14 days: <=134 U/L 15 days – <1yr: <=57 U/L 1 - <7 yrs: <=38 U/L 7 - <12 yrs: <=32 U/L Female: 12 - <19 yrs: <=24 U/L Male: 12 - <19 yrs: <=32 U/L Adult: >= 19 yrs: 13 - 39 U/L

Outreach CPTs

CPT	Modifier (if needed)	Quantity	Description	Comments
84450

Synonyms/Keywords

SGOT, AST, GOT, Aspartrate Transaminase

Ordering Applications

Ordering Application	Description
Centricity	a. AST (GOT) (Aspartrate Amino Transaminase) b. GOT (AST, SGOT) c. SGOT (AST, GOT)
Cerner	AST (COMMON)
COM	AST (SGOT)

Ordering Application

Description

Centricity

a. AST (GOT) (Aspartrate Amino Transaminase)

b. GOT (AST, SGOT)

c. SGOT (AST, GOT)

Cerner

AST (COMMON)

COM

AST (SGOT)

If the ordering application you are looking for is not listed, contact your local laboratory for assistance.

Specimen Requirements

Fasting Required	Specimen Type	Preferred Container/Tube	Acceptable Container/Tube	Specimen Volume	Specimen Minimum Volume (allows for 1 repeat)	Pediatric Minimum Volume (no repeat)
No	Plasma/Serum	Lithium-heparin Plasma Separator (PST), Serum Separator Tube (SST)	Lithium or Sodium-heparin Green Top (GTT), Red Top Tube (RTT)	1 mL	0.3 mL	0.7 mL whole blood

Collection Processing

Specimen Stability Information

Specimen Type	Temperature	Time
Plasma/Serum	Ambient	5 days
	Refrigerate	7 days
	Frozen	>7 days to 3 months

Rejection Criteria

Grossly hemolyzed samples
Samples collected with sodium citrate, potassium oxalate, or sodium fluoride preservatives

Interference

Hemolysis will falsey increase AST result.

Sulfasalazine may interfere with Dimension AST assays (Regional labs) and cause falsely depressed results.

For patients receiving treatment, venipuncture should occur prior to drug administration to prevent this interference.

Useful For

AST is used primarily in the diagnosis of liver and heart muscle disease. AST is also elevated in 97% of myocardial infarction patients.

Reference Range Information

Performing Location	Reference Range
All Performing Sites using Dimension	0 - 7 days: 20-96 IU/L 8 days - 3 years: 16-58 IU/L 4 years - 6 years: 10-47 IU/L Male: 7 - 15 years: 10-36 IU/L Male: >=16 years: 15-46 IU/L Female: 7 - 19 years: 10-36 IU/L Female: >= 20 years: 14-41 IU/L
Marshfield	Both: 0 - 14 days: <=134 U/L 15 days – <1yr: <=57 U/L 1 - <7 yrs: <=38 U/L 7 - <12 yrs: <=32 U/L Female: 12 - <19 yrs: <=24 U/L Male: 12 - <19 yrs: <=32 U/L Adult: >= 19 yrs: 13 - 39 U/L

For more information visit:

http://labtestsonline.org

Performing Laboratory Information

Performing Location	Day(s) Test Performed	Analytical Time	Methodology/Instrumentation
Chippewa Falls	Monday through Friday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Diagnostic Treatment Center	Monday through Sunday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Eau Claire	Monday through Sunday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Flambeau Hospital	Monday through Sunday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Ladysmith Medical Center	Monday through Sunday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Lake Hallie	Monday through Sunday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Lakeview Medical Center	Monday through Sunday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Marshfield	Monday through Sunday	Less than 2 hours	(IFCC), UV/NADH Rate Assay/Beckman AU
Merrill	Monday through Saturday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Minocqua	Monday through Sunday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Neillsville	Monday through Sunday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Stevens Point	Monday through Friday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Wausau	Monday through Sunday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension
Wisconsin Rapids	Monday through Friday	Less than 2 hours	Biochromatic Rate Technique/Siemens Dimension

For billing questions, see Contacts

Outreach CPTs

CPT	Modifier (if needed)	Quantity	Description	Comments
84450

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For most current information refer to the Marshfield Laboratory online reference manual.

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22340 Aspartate Amino Transferase