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22346 Benzodiazepines, Urine

Benzodiazepines, Urine
Test Code: BENZ
Synonyms/Keywords
Clonazepam, Temazepam, Diazepam, Chlordiazepoxide, Alprazolam, Flunitrazepam, Flurazepam, Nordiazepam, Chlorazepate, Lorazepam, Xanax, Librium, Valium, Ativan, Serax, Restoril​, Date Rape Drug, 7-Aminoflunitrazepam, Roofies, Rohypnol
Test Components
​The immunoassay screening test for the Benzodiazepines drug class does not detect Lorazepam or Clonazepam well.  If either of these drugs are suspected, please order the test “Benzodiazepines, Urine, Confirm Only by LC/MSMS” in addition to this test to insure detection if present.
Useful For
Detecting exposure to benzodiazepines to monitor compliance in taking prescribed medications or to detect abuse​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Urine​ Sage urine collection container​ Sterile plastic container with no preservatives​ 5 mL​ 3 mL​ 1.5 mL
Specimen Stability Information
Specimen Type Temperature Time
Urine​ Ambient​ 72 hours​
Refrigerate (Preferred)​ 30 days​
Frozen​ 30 days​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​
If no confirmatory work is required: 1 day​
 
If confirmatory work is required: 4 days
 Beckman AU680 Clinical Chemistry System with Emit Immunoassay and Liquid Chromatography/Tandem Mass Spectrometry (LCMS/MS) using Multiple Reaction Monitoring (if needed for confirmation)​
Test Information
This test was developed and its performance characteristics determined by Marshfield Labs. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) to perform high complexity testing.

This test is a screen for the Benzodiazepine drug group by immunoassay; if the screen is positive, this test automatically includes LCMS/MS confirmation for the benzodiazepines drug group. Quantitation/confirmation charged separately if needed.  It is intended for use only in patients where an initial drug screening test has not already been performed (i.e. Point-of-Care drug screening cup, dipstick, etc.). The test will detect the presence of desalkylflurazepam, nordiazepam, lorazepam, 7-aminoflunitrazepam, oxazepam, temazepam, 7-aminoclonazepam, alpha-hydroxyalprazolam, and diazepam.
 
The immunoassay screening test for the Benzodiazepines drug class does not detect Lorazepam or Clonazepam well.  If either of these drugs are suspected, please order the test “Benzodiazepines, Urine, Confirm Only by LC/MSMS” in addition to this test to insure detection if present.
Reference Range Information
Performing Location Reference Range
Marshfield​

Negative.  Positives are reported with a quantitative LC-MS/MS result.

Interpretation
The benzodiazepine group metabolizes quickly and thus, many of the parent drugs are generally not detected in urine. The detection of a metabolite(s) of the parent drug confirm the use of the drug. Below is a description of some of the more commonly prescribed benzodiazepines and the metabolites that would be present in urine. Please call the lab at ext 93734 or 800-331-3734 with any further interpretive issues.
 
Alprazolam metabolizes to alpha-hydroxyalprazolam.
Clonazepam (Klonopin) metabolizes to 7-aminoclonazepam.
Flunitrazepam (Rohypnol) metabolizes to 7-aminoflunitrazepam.
Chlordiazepoxide metabolizes to nordiazepam and oxazepam.
Chlorazepate metabolizes to nordiazepam and oxazepam.
Diazepam (Valium) metabolizes to nordiazepam, temazepam and oxazepam.
Temazepam metabolizes to oxazepam.​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
80307 ​ 1​ Single Drug Class​ ​
80346 ​ 1​ Benzodiazepine Confirmation by LCMS/MS ​If needed
​G0480 ​ ​1 ​Benzodiazepine Confirmation by LCMS/MS ​For Medicare/Medicaid, If needed
Classification
This test was developed and its performance characteristics determined by Marshfield Labs. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) to perform high complexity testing.
​
Synonyms/Keywords
Clonazepam, Temazepam, Diazepam, Chlordiazepoxide, Alprazolam, Flunitrazepam, Flurazepam, Nordiazepam, Chlorazepate, Lorazepam, Xanax, Librium, Valium, Ativan, Serax, Restoril​, Date Rape Drug, 7-Aminoflunitrazepam, Roofies, Rohypnol
Test Components
​The immunoassay screening test for the Benzodiazepines drug class does not detect Lorazepam or Clonazepam well.  If either of these drugs are suspected, please order the test “Benzodiazepines, Urine, Confirm Only by LC/MSMS” in addition to this test to insure detection if present.
Ordering Applications
Ordering Application Description
​Centricity ​Benzodiazepine Screen
​Cerner ​Benzodiazepine Screen Urine
​COM ​Benzodiazepine, Urine
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Urine​ Sage urine collection container​ Sterile plastic container with no preservatives​ 5 mL​ 3 mL​ 1.5 mL
Specimen Stability Information
Specimen Type Temperature Time
Urine​ Ambient​ 72 hours​
Refrigerate (Preferred)​ 30 days​
Frozen​ 30 days​
Useful For
Detecting exposure to benzodiazepines to monitor compliance in taking prescribed medications or to detect abuse​
Test Components
​The immunoassay screening test for the Benzodiazepines drug class does not detect Lorazepam or Clonazepam well.  If either of these drugs are suspected, please order the test “Benzodiazepines, Urine, Confirm Only by LC/MSMS” in addition to this test to insure detection if present.
Reference Range Information
Performing Location Reference Range
Marshfield​

Negative.  Positives are reported with a quantitative LC-MS/MS result.

Interpretation
The benzodiazepine group metabolizes quickly and thus, many of the parent drugs are generally not detected in urine. The detection of a metabolite(s) of the parent drug confirm the use of the drug. Below is a description of some of the more commonly prescribed benzodiazepines and the metabolites that would be present in urine. Please call the lab at ext 93734 or 800-331-3734 with any further interpretive issues.
 
Alprazolam metabolizes to alpha-hydroxyalprazolam.
Clonazepam (Klonopin) metabolizes to 7-aminoclonazepam.
Flunitrazepam (Rohypnol) metabolizes to 7-aminoflunitrazepam.
Chlordiazepoxide metabolizes to nordiazepam and oxazepam.
Chlorazepate metabolizes to nordiazepam and oxazepam.
Diazepam (Valium) metabolizes to nordiazepam, temazepam and oxazepam.
Temazepam metabolizes to oxazepam.​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​
If no confirmatory work is required: 1 day​
 
If confirmatory work is required: 4 days
 Beckman AU680 Clinical Chemistry System with Emit Immunoassay and Liquid Chromatography/Tandem Mass Spectrometry (LCMS/MS) using Multiple Reaction Monitoring (if needed for confirmation)​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
80307 ​ 1​ Single Drug Class​ ​
80346 ​ 1​ Benzodiazepine Confirmation by LCMS/MS ​If needed
​G0480 ​ ​1 ​Benzodiazepine Confirmation by LCMS/MS ​For Medicare/Medicaid, If needed
Classification
This test was developed and its performance characteristics determined by Marshfield Labs. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) to perform high complexity testing.
​
For most current information refer to the Marshfield Laboratory online reference manual.