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22528 Hemoglobin F, Quantitative (HBF)

Hemoglobin F, Quantitative (HBF)
Test Code: MISC
Synonyms/Keywords
​Lab Ref Code:  8269, Hemoglobin-F, Alkali Resistant
Useful For
Quantitating the percent of fetal hemoglobin present
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Whole Blood​ ​EDTA Lavendar Top Tube (LTT) 3 mL​ ​1 mL
Specimen Stability Information
Specimen Type Temperature Time
​Whole blood ​Refrigerated ​7 days
Rejection Criteria
Hemolysis
Mild OK; Gross OK
Specimen cannot be frozen.​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Mayo Medical Laboratories​​
Monday through Saturday
1 day​
Ion-Exchange/High-Performance Liquid Chromatography (HPLC)
Reference Lab
Test Information
Consult pathologist if patient is less than 6 months of age.​
Reference Range Information
Reference Range
1-30 days: 22.8-92.0%
1-2 months: 7.6-89.8%
3-5 months: 1.6-42.2%
6-8 months: 0.0-16.7%
9-12 months: 0.0-10.5%
13-17 months: 0.0-7.9%
18-23 months: 0.0-6.3%
> or =24 months: 0.0-0.9%
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​83021
Synonyms/Keywords
​Lab Ref Code:  8269, Hemoglobin-F, Alkali Resistant
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Whole Blood​ ​EDTA Lavendar Top Tube (LTT) 3 mL​ ​1 mL
Specimen Stability Information
Specimen Type Temperature Time
​Whole blood ​Refrigerated ​7 days
Rejection Criteria
Hemolysis
Mild OK; Gross OK
Specimen cannot be frozen.​
Useful For
Quantitating the percent of fetal hemoglobin present
Reference Range Information
Reference Range
1-30 days: 22.8-92.0%
1-2 months: 7.6-89.8%
3-5 months: 1.6-42.2%
6-8 months: 0.0-16.7%
9-12 months: 0.0-10.5%
13-17 months: 0.0-7.9%
18-23 months: 0.0-6.3%
> or =24 months: 0.0-0.9%
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Mayo Medical Laboratories​​
Monday through Saturday
1 day​
Ion-Exchange/High-Performance Liquid Chromatography (HPLC)
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​83021
For most current information refer to the Marshfield Laboratory online reference manual.