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22562 DIC Profile

DIC Profile
Test Code: DIC
Synonyms/Keywords
DIC, Disseminated Intravascular Coag. Profile​
Test Components
PT(INR), aPTT, Fibrinogen, D-dimer, Platelet count​
Specimen Requirements
See Collection and Processing Instructions section for Specimen Requirements
Collection Processing Instructions
This test requires three samples:
1. 1.0 mL 3.2% citrated plasma - blue top tube, (BTT) used for PT, APTT, and FIB.
2. 1 mL 3.2% citrated platelet poor plasma - blue top tube, (BTT) used for D-dimer.
3. 2 mL EDTA whole blood - lavender top tube, (LTT) used for platelet determination.
 
Minimum volume:
1. 1.0 mL 3.2% citrated plasma - blue top tube, (BTT)
2. 0.5 mL 3.2% citrated platelet poor plasma - blue top tube, (BTT)
3. 1 mL EDTA whole blood - lavender top tube, (LTT) and 2 unfixed smears 
 
Processing Instructions:
Blue top tubes:
- Must be drawn to the fill line.
- Invert tubes completely 3-4 times to mix.
- Do not shake.
 
First blue top tube:
- Centrifuge at 3000 rpm for 10 minutes with the brake OFF as soon as possible after collection.
- Perform testing within one hour of sample collection or separate citrated plasma for storage.
- Use plastic pipette to transfer top half of plasma to polypropylene tube.
- Avoid platelet contamination.
- Cap tightly.
 
 Second blue top tube:
-See "Preparation of Platelet Poor Plasma"
 
 EDTA lavender top tube:
-Test should be performed within 24 hours of collection.
- If not performed within 4 hours of collection, two smears should be prepared immediately for use in confirming abnormal counts.
Specimen Stability Information
Specimen Type Temperature Time
Citrated Whole Blood​ Ambient ​ 4 hours​
Citrated Plasma​
Frozen (-18°C)​
 2 weeks​
Frozen (-70°C)​ 6 months​
Thawed frozen​ 2 hours​
EDTA whole blood​ Ambient < 12 hours​
Refrigerate < 24 hours​
Unfixed blood smears Ambient ​
Rejection Criteria
BTT <90% full
Clotted samples​
Frozen whole blood​
Known IV fluid or Hickman line fluid contamination​
Gross hemolysis​

Specimens with abnormal PT and/or APTT results that have  a Hct >55% that have not had the citrate anticoagulant adjusted​

Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Diagnostic Treatment Center​ ​ ​ ​PT(INR), aPTT, Fibrinogen: Monday through Sunday​ ​Less than 2 hours ​
Optical Clot Detection/Sysmex CS 2500​
​D-dimer: Monday through Sunday​ ​Less than 2 hours ​Immunoassay/Sysmex CS 2500​
​Platelet count: Monday through Sunday​ ​Less than 2 hours ​
Electrical Impediance/Beckman Coulter DxH600 and Light Microscopy
Eau Claire
​ ​		 PT(INR), aPTT, Fibrinogen: Monday through Sunday​ Less than 2 hours​
Optical Clot Detection/Sysmex CS 2500​
D-dimer: Monday through Sunday​ Less than 2 hours​
Immunoassay/Sysmex CS 2500​
​
Platelet count: Monday through Sunday​ Less than 2 hours​
Electrical Impediance/Beckman Coulter DxH600 and Light Microscopy
Flambeau Hospital​ ​ ​ ​PT(INR), aPTT, Fibrinogen: Monday through Sunday​ ​Less than 2 hours​ Optical Clot Detection/Sysmex CS 2500​
​D-dimer: Monday through Sunday​ ​Less than 2 hours​ ​Immunoassay/Sysmex CS 2500​
​Platelet count: Monday through Sunday​ ​Less than 2 hours​ ​Electrical Impediance/Beckman Coulter DxH600 and Light Microscopy
Lakeview Medical Center​ ​ ​ ​​PT(INR), aPTT, Fibrinogen: Monday through Sunday​ ​​Less than 2 hours​ ​Optical Clot Detection/Sysmex CS 2500​​
​​D-dimer: Monday through Sunday​ ​​Less than 2 hours​ ​​Immunoassay/Sysmex CS 2500​
​Platelet count: Monday through Sunday​ ​​Less than 2 hours​ ​​Electrical Impediance/Beckman Coulter DxH600 and Light Microscopy
Marshfield​ ​ ​ ​PT(INR), aPTT, Fibrinogen: Monday through Sunday​ ​Less than 2 hours​ ​Optical Clot Detection/Sysmex CS 2500​
​D-dimer: Monday through Sunday​ ​Less than 2 hours​ ​Immunoassay/Sysmex CS 2500​
​Platelet count: Monday through Sunday​ ​Less than 2 hours​ Unicel Beckman Coulter DxH800 and Light Microscopy.​
Test Information
​Moderate hemolysis does not interfere with sample analysis, however it may be an indicator of clotting factor activation prior to testing.  Clinical correlation with results recommended.
Reference Range Information
Performing Location Reference Range
All Performing Sites See individual test listings for reference ranges.​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
85610  ​ ​ ​ PT(INR)​ ​
85730  ​ ​ ​ APTT​ ​
85384 ​ ​ ​ Fibrinogen​ ​
85379 ​ ​ ​ D-dimer​ ​
85049​ ​ ​ Platelet count​ ​
Synonyms/Keywords
DIC, Disseminated Intravascular Coag. Profile​
Test Components
PT(INR), aPTT, Fibrinogen, D-dimer, Platelet count​
Ordering Applications
Ordering Application Description
​Centricity

a. DIC

b. DIC Panel​

​Cerner ​DIC Profile
​COM ​DIC Profile
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
See Collection and Processing Instructions section for Specimen Requirements
Collection Processing
This test requires three samples:
1. 1.0 mL 3.2% citrated plasma - blue top tube, (BTT) used for PT, APTT, and FIB.
2. 1 mL 3.2% citrated platelet poor plasma - blue top tube, (BTT) used for D-dimer.
3. 2 mL EDTA whole blood - lavender top tube, (LTT) used for platelet determination.
 
Minimum volume:
1. 1.0 mL 3.2% citrated plasma - blue top tube, (BTT)
2. 0.5 mL 3.2% citrated platelet poor plasma - blue top tube, (BTT)
3. 1 mL EDTA whole blood - lavender top tube, (LTT) and 2 unfixed smears 
 
Processing Instructions:
Blue top tubes:
- Must be drawn to the fill line.
- Invert tubes completely 3-4 times to mix.
- Do not shake.
 
First blue top tube:
- Centrifuge at 3000 rpm for 10 minutes with the brake OFF as soon as possible after collection.
- Perform testing within one hour of sample collection or separate citrated plasma for storage.
- Use plastic pipette to transfer top half of plasma to polypropylene tube.
- Avoid platelet contamination.
- Cap tightly.
 
 Second blue top tube:
-See "Preparation of Platelet Poor Plasma"
 
 EDTA lavender top tube:
-Test should be performed within 24 hours of collection.
- If not performed within 4 hours of collection, two smears should be prepared immediately for use in confirming abnormal counts.
Specimen Stability Information
Specimen Type Temperature Time
Citrated Whole Blood​ Ambient ​ 4 hours​
Citrated Plasma​
Frozen (-18°C)​
 2 weeks​
Frozen (-70°C)​ 6 months​
Thawed frozen​ 2 hours​
EDTA whole blood​ Ambient < 12 hours​
Refrigerate < 24 hours​
Unfixed blood smears Ambient ​
Rejection Criteria
BTT <90% full
Clotted samples​
Frozen whole blood​
Known IV fluid or Hickman line fluid contamination​
Gross hemolysis​

Specimens with abnormal PT and/or APTT results that have  a Hct >55% that have not had the citrate anticoagulant adjusted​

Test Components
PT(INR), aPTT, Fibrinogen, D-dimer, Platelet count​
Reference Range Information
Performing Location Reference Range
All Performing Sites See individual test listings for reference ranges.​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Diagnostic Treatment Center​ ​ ​ ​PT(INR), aPTT, Fibrinogen: Monday through Sunday​ ​Less than 2 hours ​
Optical Clot Detection/Sysmex CS 2500​
​D-dimer: Monday through Sunday​ ​Less than 2 hours ​Immunoassay/Sysmex CS 2500​
​Platelet count: Monday through Sunday​ ​Less than 2 hours ​
Electrical Impediance/Beckman Coulter DxH600 and Light Microscopy
Eau Claire
​ ​		 PT(INR), aPTT, Fibrinogen: Monday through Sunday​ Less than 2 hours​
Optical Clot Detection/Sysmex CS 2500​
D-dimer: Monday through Sunday​ Less than 2 hours​
Immunoassay/Sysmex CS 2500​
​
Platelet count: Monday through Sunday​ Less than 2 hours​
Electrical Impediance/Beckman Coulter DxH600 and Light Microscopy
Flambeau Hospital​ ​ ​ ​PT(INR), aPTT, Fibrinogen: Monday through Sunday​ ​Less than 2 hours​ Optical Clot Detection/Sysmex CS 2500​
​D-dimer: Monday through Sunday​ ​Less than 2 hours​ ​Immunoassay/Sysmex CS 2500​
​Platelet count: Monday through Sunday​ ​Less than 2 hours​ ​Electrical Impediance/Beckman Coulter DxH600 and Light Microscopy
Lakeview Medical Center​ ​ ​ ​​PT(INR), aPTT, Fibrinogen: Monday through Sunday​ ​​Less than 2 hours​ ​Optical Clot Detection/Sysmex CS 2500​​
​​D-dimer: Monday through Sunday​ ​​Less than 2 hours​ ​​Immunoassay/Sysmex CS 2500​
​Platelet count: Monday through Sunday​ ​​Less than 2 hours​ ​​Electrical Impediance/Beckman Coulter DxH600 and Light Microscopy
Marshfield​ ​ ​ ​PT(INR), aPTT, Fibrinogen: Monday through Sunday​ ​Less than 2 hours​ ​Optical Clot Detection/Sysmex CS 2500​
​D-dimer: Monday through Sunday​ ​Less than 2 hours​ ​Immunoassay/Sysmex CS 2500​
​Platelet count: Monday through Sunday​ ​Less than 2 hours​ Unicel Beckman Coulter DxH800 and Light Microscopy.​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
85610  ​ ​ ​ PT(INR)​ ​
85730  ​ ​ ​ APTT​ ​
85384 ​ ​ ​ Fibrinogen​ ​
85379 ​ ​ ​ D-dimer​ ​
85049​ ​ ​ Platelet count​ ​
For most current information refer to the Marshfield Laboratory online reference manual.