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22611 Immune Deficiency Cell markers, Blood

Immune Deficiency Cell markers, Blood
Test Code: T-BCELL
Synonyms/Keywords
Flow Cytometry, T&B Immunophenotyping, B & T Cells​
Useful For
For determining percentages and absolute counts of human helper/inducer (CD4) T-lymphocytes, suppressor/cytotoxic (CD8) T-lymphocytes, T-lymphocytes (CD3), natural killer lymphocytes (CD16+56), and B lymphocytes (CD19).​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Whole blood​ EDTA Lavender Top Tube (LTT) or
EDTA Pink Top Tube (PTT)​		 ​ 1 mL​ 1 mL​ 0.5 mL​
Collection Processing Instructions
A CBC with differential is required within 4 hours of collection of specimen for this test.  Submit results with the specimen for this test or include an order for a CBC with differential.  Also submit 2 unstained smears.  Specimen must arrive in Flow Cytometry within 36 hours of collection. Notify Flow Cytometry Lab (800-222-5835, ext 16385) before sending the specimen.​
Specimen Stability Information
Specimen Type Temperature Time
Whole blood​ Room temperature​ 36 hours​
Rejection Criteria
Refrigerated or frozen specimens
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday, days only​ 1 day​ Flow Cytometry​
Test Information

Results reported include CD3 (T-cell total); T-cell absolute CD4 and CD8, including ratio; CD19 (B-cell total); and CD16+56 (Natural killer cells).​

Absolute counts for this test are based on the CBC results provided at the time of testing, including lymphocyte percentage.

Reference Range Information
Performing Location Reference Range
Marshfield​ Age appropriate ranges printed on report​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86359​ ​ ​ T-cells​ ​
86360​ ​ ​ Absolute CD4 and CD8 ​ ​
86357​ ​ ​ Natural killer cells​ ​
86355​ ​ ​ B cells ​ ​
Synonyms/Keywords
Flow Cytometry, T&B Immunophenotyping, B & T Cells​
Ordering Applications
Ordering Application Description
​Centricity ​Immune Defic. Markers
​Cerner Immune Deficiency Cell Markers​
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Whole blood​ EDTA Lavender Top Tube (LTT) or
EDTA Pink Top Tube (PTT)​		 ​ 1 mL​ 1 mL​ 0.5 mL​
Collection Processing
A CBC with differential is required within 4 hours of collection of specimen for this test.  Submit results with the specimen for this test or include an order for a CBC with differential.  Also submit 2 unstained smears.  Specimen must arrive in Flow Cytometry within 36 hours of collection. Notify Flow Cytometry Lab (800-222-5835, ext 16385) before sending the specimen.​
Specimen Stability Information
Specimen Type Temperature Time
Whole blood​ Room temperature​ 36 hours​
Rejection Criteria
Refrigerated or frozen specimens
Useful For
For determining percentages and absolute counts of human helper/inducer (CD4) T-lymphocytes, suppressor/cytotoxic (CD8) T-lymphocytes, T-lymphocytes (CD3), natural killer lymphocytes (CD16+56), and B lymphocytes (CD19).​
Reference Range Information
Performing Location Reference Range
Marshfield​ Age appropriate ranges printed on report​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday, days only​ 1 day​ Flow Cytometry​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86359​ ​ ​ T-cells​ ​
86360​ ​ ​ Absolute CD4 and CD8 ​ ​
86357​ ​ ​ Natural killer cells​ ​
86355​ ​ ​ B cells ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.