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22733 Rotavirus Antigen Test

Rotavirus Antigen Test
Test Code: ROAG
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No ​ Feces​ ​ Leakproof container ​ 1 mL, pea size portion 1 mL or 0.5 g​

1 mL or

0.5 g

Collection Processing Instructions

​Stool specimens should be collected as soon after onset of symptoms as possible. Peak viral counts are reported to occur on days 3-5 after onset of symptoms. Samples collected 8 days or more after onset of symptoms may not contain enough rotavirus antigens to produce a positive reaction.

Specimen Stability Information
Specimen Type Temperature Time
​Feces ​ Wet ice or refrigerate​ Within 72 hours of collection​
-20 degrees Celsius​ Greater than 72 hours after collection​
Rejection Criteria
Samples collected in containers with media, preservatives, animal serum, or detergent
Samples collected in diapers​
​Specimens submitted on swabs
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Diagnostic Treatment Center​ ​Monday through Sunday ​Less than 2 hours​ ​ImmunoCard STAT! ROTAVIRUS
​Marshfield Monday through Friday Less than 2 hours​ Sure-Vue Rotavirus Test
Test Information
​Rotavirus infections are self-limiting. Testing is not routinely recommended in adults and adolescents.
Reference Range Information
Performing Location Reference Range
All Performing Sites Negative for Rotavirus antigen​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87425 ​ ​ ​Rotavirus ​
Ordering Applications
Ordering Application Description
​Centricity

​a. Ag. Rotavirus

b. Rotavirus Antigen

​Cerner ​Rotavirus Antigen Stool
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No ​ Feces​ ​ Leakproof container ​ 1 mL, pea size portion 1 mL or 0.5 g​

1 mL or

0.5 g

Collection Processing

​Stool specimens should be collected as soon after onset of symptoms as possible. Peak viral counts are reported to occur on days 3-5 after onset of symptoms. Samples collected 8 days or more after onset of symptoms may not contain enough rotavirus antigens to produce a positive reaction.

Specimen Stability Information
Specimen Type Temperature Time
​Feces ​ Wet ice or refrigerate​ Within 72 hours of collection​
-20 degrees Celsius​ Greater than 72 hours after collection​
Rejection Criteria
Samples collected in containers with media, preservatives, animal serum, or detergent
Samples collected in diapers​
​Specimens submitted on swabs
Reference Range Information
Performing Location Reference Range
All Performing Sites Negative for Rotavirus antigen​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Diagnostic Treatment Center​ ​Monday through Sunday ​Less than 2 hours​ ​ImmunoCard STAT! ROTAVIRUS
​Marshfield Monday through Friday Less than 2 hours​ Sure-Vue Rotavirus Test
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87425 ​ ​ ​Rotavirus ​
For most current information refer to the Marshfield Laboratory online reference manual.