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22749 HIV-1 Antibody Confirm by Western Blot (HV1WB)

HIV-1 Antibody Confirm by Western Blot (HV1WB)
Test Code: WBLOT
Synonyms/Keywords
​Ref Lab Code: 36006, Acquired Immune Deficiency Syndrome (AIDS), Anti-HIV, Anti-HIV-1 Western blot, HIV Types 1 Antibodies, HIV-1 Western blot, HIV-1 Ab Western blot, HIV-1 Antibodies, HUman Immunodeficiency Virus (HIV), Western blot
Useful For
Confirmatory detection of HIV-1 antibodies in patients with reactive HIV-1 antibody results from HIV 1,2 immunoassays.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​Serum Serum Separator Tube (SST)​ ​Red Top Tube (RTT) 1.6 mL​ 0.5 mL​ ​
Collection Processing Instructions
Separate serum from the blood within 24 hours of collection. Recommended guidelines for optimal sample processing should be followed. Specimen must be free of particulate matter including fibrin. Specimens transported to Marshfield must be removed from the primary collection tube and sent in a labeled daughter tube and sent frozen.
Specimen Stability Information
Specimen Type Temperature Time
Serum​ Frozen at -20 deg Celsius (preferred) ​ 30 days​
​ ​Refrigerate ​7 days
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Mayo Medical Laboratories​
 Monday through Friday​
1 day​
 Western Blot​
Reference Lab
Mayo Clinic Laboratories
Reference Range Information
Negative
Interpretation

An HIV-1 antibody Western Blot (WB) result is interpreted as positive when at least 2 of the 3 following bands are present: p24, gp41, and gp120/160. A positive HIV-1 antibody WB result following a reactive HIV-1/-2 antibody screening immunoassay (IA) or reactive rapid HIV-1/-2 antibody IA test supports HIV-1 infection at an undetermined stage of disease. Another specimen should be promptly submitted for HIV-1 Viral Load (HIVPCR), HIV-1 Proviral DNA by PCR, Qual (PHIV), or a repeat HIV-1 WB on first-time HIV-1 positive individuals to verify the diagnosis.

Indeterminate WB patterns can be found in up to 15% of persons without evidence of HIV infection. Repeatedly indeterminate results should be followed up with HIV-1 Viral Load (HIVPCR) or HIV-1 Proviral DNA by PCR, Qual (PHIV).

This test does not reliably detect anti-HIV-2 antibodies. HIV-2 is rare in most USA populations, but is linked with individuals from certain West African countries or their contacts. Should HIV-2 be suspected either clinically or due to a positive HIV-2 result from an HIV-1/-2 differentiation IA, please contact Marshfield Labs Customer Service for information on HIV-2 confirmatory testing. HIV infection is a nationally notifiable disease.

Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86689​ ​ ​ ​
Synonyms/Keywords
​Ref Lab Code: 36006, Acquired Immune Deficiency Syndrome (AIDS), Anti-HIV, Anti-HIV-1 Western blot, HIV Types 1 Antibodies, HIV-1 Western blot, HIV-1 Ab Western blot, HIV-1 Antibodies, HUman Immunodeficiency Virus (HIV), Western blot
Ordering Applications
Ordering Application Description
​Centricity ​HIV-1,2 AbEIA w/Confirm Eval (WBAR)
​Cerner ​HIV 1,2 Antibody-Confirm Eval (WBAR)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​Serum Serum Separator Tube (SST)​ ​Red Top Tube (RTT) 1.6 mL​ 0.5 mL​ ​
Collection Processing
Separate serum from the blood within 24 hours of collection. Recommended guidelines for optimal sample processing should be followed. Specimen must be free of particulate matter including fibrin. Specimens transported to Marshfield must be removed from the primary collection tube and sent in a labeled daughter tube and sent frozen.
Specimen Stability Information
Specimen Type Temperature Time
Serum​ Frozen at -20 deg Celsius (preferred) ​ 30 days​
​ ​Refrigerate ​7 days
Useful For
Confirmatory detection of HIV-1 antibodies in patients with reactive HIV-1 antibody results from HIV 1,2 immunoassays.
Reference Range Information
Negative
Interpretation

An HIV-1 antibody Western Blot (WB) result is interpreted as positive when at least 2 of the 3 following bands are present: p24, gp41, and gp120/160. A positive HIV-1 antibody WB result following a reactive HIV-1/-2 antibody screening immunoassay (IA) or reactive rapid HIV-1/-2 antibody IA test supports HIV-1 infection at an undetermined stage of disease. Another specimen should be promptly submitted for HIV-1 Viral Load (HIVPCR), HIV-1 Proviral DNA by PCR, Qual (PHIV), or a repeat HIV-1 WB on first-time HIV-1 positive individuals to verify the diagnosis.

Indeterminate WB patterns can be found in up to 15% of persons without evidence of HIV infection. Repeatedly indeterminate results should be followed up with HIV-1 Viral Load (HIVPCR) or HIV-1 Proviral DNA by PCR, Qual (PHIV).

This test does not reliably detect anti-HIV-2 antibodies. HIV-2 is rare in most USA populations, but is linked with individuals from certain West African countries or their contacts. Should HIV-2 be suspected either clinically or due to a positive HIV-2 result from an HIV-1/-2 differentiation IA, please contact Marshfield Labs Customer Service for information on HIV-2 confirmatory testing. HIV infection is a nationally notifiable disease.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Mayo Medical Laboratories​
 Monday through Friday​
1 day​
 Western Blot​
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86689​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.