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23099 Herpes Simplex Virus (HSV) 1/2 by Rapid PCR

Herpes Simplex Virus (HSV) 1/2 by Rapid PCR
Test Code: HS12PCR
Synonyms/Keywords
​HSV by PCR, HSAG, VAG, DFA Antigen
Useful For
​Useful for detection and differentiation of HSV I and II DNA in CSF and vesicular fluid and cellular material from base of lesion (skin, mucosal, urogenital).
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ ​CSF ​Sterile vial ​ ​1.0 mL ​0.5 mL ​0.25 mL
​No ​Swab
Viral transport media​
(M4RT or M6)
 ​ 1 swab​ ​ ​
Collection Processing Instructions
Swabs in viral transport media: Vesicular fluid and cellular material from the base of lesion (skin, mucosal, urogenital). Specimens from early stage vesicular lesions rather than ulcerative or crusted lesions should be obtained. Place swabs in viral transport medium and break off swab at breakpoint of shaft.

For more information on Specimen Collection Media/Swab, see Specimen Transport Pictorial.
Specimen Stability Information
Specimen Type Temperature Time
CSF​ Refrigerate​ ​7 days
​Frozen ​30 days
​Swab ​Refrigerate ​7 days
Rejection Criteria
Collected on Wood-Shafted, Cotton, or Calcium Alginate Swabs
Urine and Body Fluids (with the exception of CSF)​
​Solid Tissue Samples
​Frozen Swabs
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Lesions: ​Monday - Friday
CSF: Monday - Sunday		 ​6-8 hours* ​PCR/Hybridization Probe
*Testing performed once per day.  
Samples must be received in the lab by 0400 to be tested same day.
Test Information
This test was developed and its performance characteristics determined by Marshfield Labs.  It has not been cleared or approved by the U.S. Food and Drug Administration.  The FDA has determined that such clearance or approval is not necessary.  This test is for clinical purposes.  It should not be regarded as investigational or for research.  This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity clinical testing.
Reference Range Information
Performing Location Reference Range
​Marshfield ​Negative
Interpretation
​Negative: No Herpes Simplex Virus DNA detected by Rapid PCR Positives: Herpes simplex type 1 DNA detected or Herpes simplex type 2 DNA detected
- All positive HS12PCR (CSF) results are called to the provider.
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87529 ​ ​ ​ ​
Classification
This test was developed and its performance characteristics determined by Marshfield Labs.  It has not been cleared or approved by the U.S. Food and Drug Administration.  The FDA has determined that such clearance or approval is not necessary.  This test is for clinical purposes.  It should not be regarded as investigational or for research.  This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity clinical testing.​
Synonyms/Keywords
​HSV by PCR, HSAG, VAG, DFA Antigen
Ordering Applications
Ordering Application Description
​Centricity ​HSV by PCR
​Cerner Herpes Simplex Virus by PCR​
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ ​CSF ​Sterile vial ​ ​1.0 mL ​0.5 mL ​0.25 mL
​No ​Swab
Viral transport media​
(M4RT or M6)
 ​ 1 swab​ ​ ​
Collection Processing
Swabs in viral transport media: Vesicular fluid and cellular material from the base of lesion (skin, mucosal, urogenital). Specimens from early stage vesicular lesions rather than ulcerative or crusted lesions should be obtained. Place swabs in viral transport medium and break off swab at breakpoint of shaft.

For more information on Specimen Collection Media/Swab, see Specimen Transport Pictorial.
Specimen Stability Information
Specimen Type Temperature Time
CSF​ Refrigerate​ ​7 days
​Frozen ​30 days
​Swab ​Refrigerate ​7 days
Rejection Criteria
Collected on Wood-Shafted, Cotton, or Calcium Alginate Swabs
Urine and Body Fluids (with the exception of CSF)​
​Solid Tissue Samples
​Frozen Swabs
Useful For
​Useful for detection and differentiation of HSV I and II DNA in CSF and vesicular fluid and cellular material from base of lesion (skin, mucosal, urogenital).
Reference Range Information
Performing Location Reference Range
​Marshfield ​Negative
Interpretation
​Negative: No Herpes Simplex Virus DNA detected by Rapid PCR Positives: Herpes simplex type 1 DNA detected or Herpes simplex type 2 DNA detected
- All positive HS12PCR (CSF) results are called to the provider.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Lesions: ​Monday - Friday
CSF: Monday - Sunday		 ​6-8 hours* ​PCR/Hybridization Probe
*Testing performed once per day.  
Samples must be received in the lab by 0400 to be tested same day.
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87529 ​ ​ ​ ​
Classification
This test was developed and its performance characteristics determined by Marshfield Labs.  It has not been cleared or approved by the U.S. Food and Drug Administration.  The FDA has determined that such clearance or approval is not necessary.  This test is for clinical purposes.  It should not be regarded as investigational or for research.  This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity clinical testing.​
For most current information refer to the Marshfield Laboratory online reference manual.