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23208 Microorganism ID, Ribosomal RNA Sequencing

Microorganism ID, Ribosomal RNA Sequencing
Test Code: IDSEQO
Synonyms/Keywords
​16S, Broad spectrum PCR, Bacterial sequencing, 18S, ITS, Fungal sequencing
Useful For
​Intended for the identification of bacterial, yeast or fungal isolates that are not successfully identified by conventional Microbiology methods.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No ​Microorganism ​Sealed Plate Media ​Agarose Slant ​ ​ ​
Collection Processing Instructions

​Subculture bacterial, yeast or fungal isolate to non-selective media for pure growth.  Do not send primary culture plates containing mixed microbial species.  Seal plates and submit in a biohazard bag.

Note: Mixed cultures will delay analysis

Acceptable Specimen Types
​Microorganism
Specimen Stability Information
Specimen Type Temperature Time
Bacterial Isolate​ ​Room Temperature ​72 hours
​Yeast Isolate ​Room Temperature 72 hours​
​Fungal Isolate ​Room Temperature ​72 hours
Rejection Criteria
Samples submitted on Swabs
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Weekly ​4 days Ribosomal RNA gene amplification and sequence analysis​
Test Information
This test was developed and its performance characteristics determined by Marshfield Labs.  It has not been cleared or approved by the U.S. Food and Drug Administration.  The FDA has determined that such clearance or approval is not necessary.  This test is for clinical purposes.  It should not be regarded as investigational or for research.  This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity clinical testing.​
Reference Range Information
Performing Location Reference Range
​Marshfield ​See Interpretation
Interpretation

​Organism identified by 16S nucleic acid amplification.

Ribosomal RNA sequence is matched to known rRNA sequences in GenBank® (NIH, U.S. DHHS) and Ridom (Ridom GmbH, Wurzburg). Presence of two or more bacterial species may result in ambiguous rRNA sequence. Interpretation based on CLSI Guidelines: Interpretive criteria for the identification of bacteria by DNA target sequencing.

Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87153 ​ 1​ Identification by nucleic acid sequencing method​ ​
Classification
This test was developed and its performance characteristics determined by Marshfield Labs.  It has not been cleared or approved by the U.S. Food and Drug Administration.  The FDA has determined that such clearance or approval is not necessary.  This test is for clinical purposes.  It should not be regarded as investigational or for research.  This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity clinical testing.​
Synonyms/Keywords
​16S, Broad spectrum PCR, Bacterial sequencing, 18S, ITS, Fungal sequencing
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No ​Microorganism ​Sealed Plate Media ​Agarose Slant ​ ​ ​
Collection Processing

​Subculture bacterial, yeast or fungal isolate to non-selective media for pure growth.  Do not send primary culture plates containing mixed microbial species.  Seal plates and submit in a biohazard bag.

Note: Mixed cultures will delay analysis

Acceptable Specimen Types
​Microorganism
Specimen Stability Information
Specimen Type Temperature Time
Bacterial Isolate​ ​Room Temperature ​72 hours
​Yeast Isolate ​Room Temperature 72 hours​
​Fungal Isolate ​Room Temperature ​72 hours
Rejection Criteria
Samples submitted on Swabs
Useful For
​Intended for the identification of bacterial, yeast or fungal isolates that are not successfully identified by conventional Microbiology methods.
Reference Range Information
Performing Location Reference Range
​Marshfield ​See Interpretation
Interpretation

​Organism identified by 16S nucleic acid amplification.

Ribosomal RNA sequence is matched to known rRNA sequences in GenBank® (NIH, U.S. DHHS) and Ridom (Ridom GmbH, Wurzburg). Presence of two or more bacterial species may result in ambiguous rRNA sequence. Interpretation based on CLSI Guidelines: Interpretive criteria for the identification of bacteria by DNA target sequencing.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Weekly ​4 days Ribosomal RNA gene amplification and sequence analysis​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87153 ​ 1​ Identification by nucleic acid sequencing method​ ​
Classification
This test was developed and its performance characteristics determined by Marshfield Labs.  It has not been cleared or approved by the U.S. Food and Drug Administration.  The FDA has determined that such clearance or approval is not necessary.  This test is for clinical purposes.  It should not be regarded as investigational or for research.  This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity clinical testing.​
For most current information refer to the Marshfield Laboratory online reference manual.