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23215 Amnio Aneuploidy, FISH SO (PADF)

Amnio Aneuploidy, FISH SO (PADF)
Test Code: AFPADSO
Synonyms/Keywords
​Ref Lab Code: 81424, Ambiguous Genitalia, Chromosome Analysis Consultation, PAD, Down Syndrome, Edward's Syndrome, FISH (Fluorescent In Situ Hybridization), FISH 13,18,21,X,Y, FISH Amniotic Fluid, FISH for Ambiguous Genitalia, FISH for PAD, FISH for Prenatal Aneuploidy, FISH for Prenatal Aneusomy, FISH, Prenatal Aneuploidy Detection, FISH, Rule Out Down Syndrome, FISH, Rule Out Edwards Syndrome, FISH, Rule Out Klinefelter Syndrome, FISH, Rule Out Patau Syndrome, FISH, Rule Out Trisomy 13/ Trisomy 18/ or Trisomy 21, FISH, Rule Out XXY, XXX, or XYY, FISH, Turner Syndrome, Klinefelter Syndrome, Patau Syndrome, Prenatal Aneuploidy Detection (PAD), Prenatal Aneuploidy Detection, FISH, Prenatal Aneusomy Detection (PAD), Turner Syndrome, XXY, XXX, OR XYY
Useful For
​Ruling out aneuploidy of chromosomes 13, 18, 21, X, and Y in a rapid, cost-efficient manner
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Submit only 1 of the following specimens:
​ ​ ​ ​ ​ ​
Amniotic fluid​ Amniotic fluid container​ 20-25 mL​ 7 mL​
Acceptable:
Chorionic villi
15-mL tube containing 15 mL of transport media​ 20-30 mg​ 7 mg​
Collection Processing Instructions

​Provide a reason for referral and gestational age with each specimen, and verify the specimen source. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.

Amniotic fluid:

1. Optimal timing for specimen collection is during 14 to 18 weeks of gestation, but specimens collected at other weeks of gestation are also accepted. Provide gestational age at the time of amniocentesis.
2. Discard the first 2 mL of amniotic fluid.
Additional Information:
1. Place the tubes in a Styrofoam container.
2. Fill remaining space with packing material.
3. Unavoidably, about 1% to 2% of mailed-in specimens are not viable.
4. Bloody specimens are undesirable.
5. If the specimen does not grow in culture, you will be notified within 7 days of receipt.
6. Results will be reported and also telephoned or faxed, if requested.
 
Chorionic villi:
 
1. Collect specimen by the transabdominal or transcervical method.
2. Transfer chorionic villi to a Petri dish containing transport medium.
3. Using a stereomicroscope and sterile forceps, assess the quality and quantity of the villi and remove any blood clots and maternal decidua.
Specimen Stability Information
Specimen Type Temperature
Varies​ Refrigerated (preferred)​
Ambient ​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Mayo Medical Laboratories
Monday through Sunday​ 3 days​
Fluorescence In Situ Hybridization (FISH)​
Reference Lab
Reference Range Information
Interpretive report.
Interpretation
​An interpretive report is provided.
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​88291 1 Interpretation and report​
88271 ​2 Probe Set, 1ST
​88271 ​2 Probe, +2 ​as needed
​88271 ​1 ​Probe, +1 ​as needed
​88271 ​2 ​Probe +2 ​as needed
​88271 ​3 ​Probe +3 ​as needed
​88274 ​1 ​Interphases, <25 ​as needed
​88274 ​1 ​Interphases, 25-99 ​as needed
​88275 ​1 ​Interphases, >100 ​as needed
Synonyms/Keywords
​Ref Lab Code: 81424, Ambiguous Genitalia, Chromosome Analysis Consultation, PAD, Down Syndrome, Edward's Syndrome, FISH (Fluorescent In Situ Hybridization), FISH 13,18,21,X,Y, FISH Amniotic Fluid, FISH for Ambiguous Genitalia, FISH for PAD, FISH for Prenatal Aneuploidy, FISH for Prenatal Aneusomy, FISH, Prenatal Aneuploidy Detection, FISH, Rule Out Down Syndrome, FISH, Rule Out Edwards Syndrome, FISH, Rule Out Klinefelter Syndrome, FISH, Rule Out Patau Syndrome, FISH, Rule Out Trisomy 13/ Trisomy 18/ or Trisomy 21, FISH, Rule Out XXY, XXX, or XYY, FISH, Turner Syndrome, Klinefelter Syndrome, Patau Syndrome, Prenatal Aneuploidy Detection (PAD), Prenatal Aneuploidy Detection, FISH, Prenatal Aneusomy Detection (PAD), Turner Syndrome, XXY, XXX, OR XYY
Ordering Applications
Ordering Application Description
​Centricity ​Amnio Aneupolid FISH
​Cerner ​Amnio Aneuploidy FISH SO (PAD)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Submit only 1 of the following specimens:
​ ​ ​ ​ ​ ​
Amniotic fluid​ Amniotic fluid container​ 20-25 mL​ 7 mL​
Acceptable:
Chorionic villi
15-mL tube containing 15 mL of transport media​ 20-30 mg​ 7 mg​
Collection Processing

​Provide a reason for referral and gestational age with each specimen, and verify the specimen source. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.

Amniotic fluid:

1. Optimal timing for specimen collection is during 14 to 18 weeks of gestation, but specimens collected at other weeks of gestation are also accepted. Provide gestational age at the time of amniocentesis.
2. Discard the first 2 mL of amniotic fluid.
Additional Information:
1. Place the tubes in a Styrofoam container.
2. Fill remaining space with packing material.
3. Unavoidably, about 1% to 2% of mailed-in specimens are not viable.
4. Bloody specimens are undesirable.
5. If the specimen does not grow in culture, you will be notified within 7 days of receipt.
6. Results will be reported and also telephoned or faxed, if requested.
 
Chorionic villi:
 
1. Collect specimen by the transabdominal or transcervical method.
2. Transfer chorionic villi to a Petri dish containing transport medium.
3. Using a stereomicroscope and sterile forceps, assess the quality and quantity of the villi and remove any blood clots and maternal decidua.
Specimen Stability Information
Specimen Type Temperature
Varies​ Refrigerated (preferred)​
Ambient ​
Useful For
​Ruling out aneuploidy of chromosomes 13, 18, 21, X, and Y in a rapid, cost-efficient manner
Reference Range Information
Interpretive report.
Interpretation
​An interpretive report is provided.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Mayo Medical Laboratories
Monday through Sunday​ 3 days​
Fluorescence In Situ Hybridization (FISH)​
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​88291 1 Interpretation and report​
88271 ​2 Probe Set, 1ST
​88271 ​2 Probe, +2 ​as needed
​88271 ​1 ​Probe, +1 ​as needed
​88271 ​2 ​Probe +2 ​as needed
​88271 ​3 ​Probe +3 ​as needed
​88274 ​1 ​Interphases, <25 ​as needed
​88274 ​1 ​Interphases, 25-99 ​as needed
​88275 ​1 ​Interphases, >100 ​as needed
For most current information refer to the Marshfield Laboratory online reference manual.