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24890 EGFR with ALK Reflex, Tumor (EGFRR)

EGFR with ALK Reflex, Tumor (EGFRR)
Test Code:  EGFRPSO
Synonyms/Keywords
EGFR-tyrosine kinase inhibitor, Epidermal growth factor receptor, Erlotinib, Gefitinib, Non-small cell lung cancer​
Useful For
​Identifying non-small cell lung cancers that may benefit from treatment with epidermal growth factor receptor-tyrosine kinase or anaplastic lymphoma kinase inhibitors. When this test is ordered, EGFR and LCAF testing will always be ordered. The  EGFR Gene, Mutation Analysis, 29 Mutation Panel, Tumor, will always be performed.  All specimens without an EGFR mutation will be automatically reflexed to LCAF/ALK (2p23), Lung Caner, FISH Tissue at an additional charge.  Specimens with an identified EGFR mutation will result in cancellation of the LCAF test.   See - EGFR with ALK reflex.
Specimen Requirements
​
​Specimen Type ​Preferred Container/Tube ​Acceptable Container/Tube ​Specimen Volume ​Specimen Minimum Volume (allows for 1 repeat) ​Pediatric Minimum Volume (no repeat)
Tissue
​FFPE
tissue block
 Slides ​​12 unstained, positively charged, unbaked slides or 2 H&E slides and 10 unstained, positively charged, unbaked slides with 5 micron thick sections of the tumor tissue ​2 H&E slides and 10 unstained, positively charged, unbaked slides with 5 micron thick sections of the tumor tissue ​
​T​umor adequacy is 6x6 mm (5000 cells) of tumor, at least 10% tumor nuclei (can be present over multiple slides) ​ ​ ​ ​ ​
 
Collection Processing Instructions
Provide a pathology report with each specimen. The laboratory will not reject specimens that arrive without this information but will hold the specimen until a pathology report is received. Informed Consent for Genetic Testing For blocks residing at a Non-Clinic location, please complete the Surgical Pathology Specimen Request form found in FORMS PRINTER or located here: Surgical Pathology Specimen Request
Specimen Stability Information
Specimen Type Temperature
Tissue ​Ambient (preferred)
​Frozen
​Refrigerated
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Mayo Medical Laboratories​
 Monday through Friday​ 5 days (EGFR only) 10 days (if ALK is added on) Scorpions real-time PCR and allele-specific PCR technologies
Reference Lab
Mayo Clinic Laboratories
Reference Range Information
Interpretative Report
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
88381​ ​ 1​ Manual Microdissection​ ​
81235​ ​ 1​ EGFR Mutation Analysis​ ​
If ALK reflexed​ ​ ​ ​ ​
​88271 ​ ​2 ​Cytogenetics DNA Probe ​​​
​88274 ​ ​1 ​Cytogenetics 25-99 ​
​88291 ​ ​1 ​Cytogenetics Interp and Report ​
Synonyms/Keywords
EGFR-tyrosine kinase inhibitor, Epidermal growth factor receptor, Erlotinib, Gefitinib, Non-small cell lung cancer​
Ordering Applications
Ordering Application Description
​Centricity ​EGFR Mutation Analysis
​Cerner None​
COM ​EGFR with ALK Reflex
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
​
​Specimen Type ​Preferred Container/Tube ​Acceptable Container/Tube ​Specimen Volume ​Specimen Minimum Volume (allows for 1 repeat) ​Pediatric Minimum Volume (no repeat)
Tissue
​FFPE
tissue block
 Slides ​​12 unstained, positively charged, unbaked slides or 2 H&E slides and 10 unstained, positively charged, unbaked slides with 5 micron thick sections of the tumor tissue ​2 H&E slides and 10 unstained, positively charged, unbaked slides with 5 micron thick sections of the tumor tissue ​
​T​umor adequacy is 6x6 mm (5000 cells) of tumor, at least 10% tumor nuclei (can be present over multiple slides) ​ ​ ​ ​ ​
 
Collection Processing
Provide a pathology report with each specimen. The laboratory will not reject specimens that arrive without this information but will hold the specimen until a pathology report is received. Informed Consent for Genetic Testing For blocks residing at a Non-Clinic location, please complete the Surgical Pathology Specimen Request form found in FORMS PRINTER or located here: Surgical Pathology Specimen Request
Specimen Stability Information
Specimen Type Temperature
Tissue ​Ambient (preferred)
​Frozen
​Refrigerated
Useful For
​Identifying non-small cell lung cancers that may benefit from treatment with epidermal growth factor receptor-tyrosine kinase or anaplastic lymphoma kinase inhibitors. When this test is ordered, EGFR and LCAF testing will always be ordered. The  EGFR Gene, Mutation Analysis, 29 Mutation Panel, Tumor, will always be performed.  All specimens without an EGFR mutation will be automatically reflexed to LCAF/ALK (2p23), Lung Caner, FISH Tissue at an additional charge.  Specimens with an identified EGFR mutation will result in cancellation of the LCAF test.   See - EGFR with ALK reflex.
Reference Range Information
Interpretative Report
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Mayo Medical Laboratories​
 Monday through Friday​ 5 days (EGFR only) 10 days (if ALK is added on) Scorpions real-time PCR and allele-specific PCR technologies
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
88381​ ​ 1​ Manual Microdissection​ ​
81235​ ​ 1​ EGFR Mutation Analysis​ ​
If ALK reflexed​ ​ ​ ​ ​
​88271 ​ ​2 ​Cytogenetics DNA Probe ​​​
​88274 ​ ​1 ​Cytogenetics 25-99 ​
​88291 ​ ​1 ​Cytogenetics Interp and Report ​
For most current information refer to the Marshfield Laboratory online reference manual.