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24911 Ova and Parasites, (Giardia/Crypt) Routine

Ova and Parasites, (Giardia/Crypt) Routine
Test Code: OPRCG
Synonyms/Keywords

Giardia/Cryptosporidium EIA
O&P, (Giardia/Crypt) Routine​

Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ ​ ​ Feces​ ​ ​ Para-Pak C&S transport or other Cary Blair medium Para-Pak 10% Formalin, or Sodium acetate formalin (SAF) preservatives​ One vial of feces in ParaPak C&S transport 5 g stool C&S transport ​
​ ​ Fresh stool in a clean, leakproof container 5 g​ 1 g​ ​
Swab - Rectal One swab ​
​Duodenal aspirate ​Fresh in a clean, leakproof container ​ ​1 mL ​ ​0.25 mL
Collection Processing Instructions

​Place fresh stool specimen into a Para-Pak C&S transport collection vial. Add sufficient stool specimen to bring the liquid in the vial to the fill line.  A single sample is recommended.

If more than one specimen is collected within a 24 hour period, only one will be accepted by the lab.

If submitting a duodenal aspirate, the source must be noted for correct testing.

For more information on Specimen Collection Media/Swab, see Specimen Transport Pictorial.

Specimen Stability Information
Specimen Type Temperature Time
Para-Pak C&S (Cary Blair)​ ​Refrigerate ​​< or = 4 days
Preserved Feces​ Room Temperature 2 months
Refrigerated
​Fresh feces, rectal swabs ​ Refrigerated ​< or = 48 hours
​Frozen ​> 48 hours
​Duodenal aspirate, fresh Room Temperature​ ​< or = 2 hours
Rejection Criteria
Feces containing barium
Merthiolate iodine formalin (MIF), PVA only​
Rectal swab, fresh feces >48 hours old if not frozen
​Unpreserved duodenal aspirates > 2 hours old
​Leaking specimens
​Feces in Para-Pak ZN-PVA Fixative only
​Feces in Total-Fix vial
​> 1 specimen collected within 24 hour period
Interference
​Barium
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Diagnostic Treatment Center ​Monday through Sunday ​Less than 2 hours​
​ProSpecT Cryptosporidium Microplate EIA
 
ProSpecT Giardia Microplate EIA 
​Marshfield Monday through Saturday excluding holidays​ Less than 2 hours​

ProSpecT Cryptosporidium Microplate EIA

ProSpecT Giardia Microplate EIA

Test Information

Cryptosporidium and Giardia are the most common protozoan parasites encountered in the US and represent 85% of all significant parasites recovered in our laboratory. Therefore, routine O & P screens only for these organisms. This EIA method had a sensitivity and specificity >95% for these two organisms. Only a single sample is recommended. Complete microscopic examination is available but should be reserved for patients who have a history of travel or residency in a developing country, AIDS patients, patients in whom roundworms or tapeworms are suspected, or patients in whom symptoms persist despite a negative test for Cryptosporidium and Giardia. See Ova and Parasites, Special if complete examination is needed.

Microscopic exam for Stongyloides will be performed in addition to the Giardia/Cryptosporidium EIA on all duodenal aspirates.

Reference Range Information
Performing Location Reference Range
All Performing Sites Negative for Cryptosporidium and Giardia​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
87328​ ​ ​ Cryptosporidium​ ​
87329 ​ ​ Giardia​ ​
Synonyms/Keywords

Giardia/Cryptosporidium EIA
O&P, (Giardia/Crypt) Routine​

Ordering Applications
Ordering Application Description
​Centricity ​O&P, Routine (Giardia/Crypt)
​Cerner ​O and P, Stool (Routine) (COMMON)
​Clinical Order Manager ​O&P, (Giardia/Cyp) Routine​
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ ​ ​ Feces​ ​ ​ Para-Pak C&S transport or other Cary Blair medium Para-Pak 10% Formalin, or Sodium acetate formalin (SAF) preservatives​ One vial of feces in ParaPak C&S transport 5 g stool C&S transport ​
​ ​ Fresh stool in a clean, leakproof container 5 g​ 1 g​ ​
Swab - Rectal One swab ​
​Duodenal aspirate ​Fresh in a clean, leakproof container ​ ​1 mL ​ ​0.25 mL
Collection Processing

​Place fresh stool specimen into a Para-Pak C&S transport collection vial. Add sufficient stool specimen to bring the liquid in the vial to the fill line.  A single sample is recommended.

If more than one specimen is collected within a 24 hour period, only one will be accepted by the lab.

If submitting a duodenal aspirate, the source must be noted for correct testing.

For more information on Specimen Collection Media/Swab, see Specimen Transport Pictorial.

Specimen Stability Information
Specimen Type Temperature Time
Para-Pak C&S (Cary Blair)​ ​Refrigerate ​​< or = 4 days
Preserved Feces​ Room Temperature 2 months
Refrigerated
​Fresh feces, rectal swabs ​ Refrigerated ​< or = 48 hours
​Frozen ​> 48 hours
​Duodenal aspirate, fresh Room Temperature​ ​< or = 2 hours
Rejection Criteria
Feces containing barium
Merthiolate iodine formalin (MIF), PVA only​
Rectal swab, fresh feces >48 hours old if not frozen
​Unpreserved duodenal aspirates > 2 hours old
​Leaking specimens
​Feces in Para-Pak ZN-PVA Fixative only
​Feces in Total-Fix vial
​> 1 specimen collected within 24 hour period
Interference
​Barium
Reference Range Information
Performing Location Reference Range
All Performing Sites Negative for Cryptosporidium and Giardia​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Diagnostic Treatment Center ​Monday through Sunday ​Less than 2 hours​
​ProSpecT Cryptosporidium Microplate EIA
 
ProSpecT Giardia Microplate EIA 
​Marshfield Monday through Saturday excluding holidays​ Less than 2 hours​

ProSpecT Cryptosporidium Microplate EIA

ProSpecT Giardia Microplate EIA

For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
87328​ ​ ​ Cryptosporidium​ ​
87329 ​ ​ Giardia​ ​
For most current information refer to the Marshfield Laboratory online reference manual.