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25203 Microsatellite Instability (MSI) by PCR (MSI)

Microsatellite Instability (MSI) by PCR (MSI)
Test Code: MSIPSO
Synonyms/Keywords
DNA Mismatch Repair-related HNPCC, Hereditary Nonpolyposis Colon Cancer (HNPCC), Lynch Syndrome, Microsatellite Instability (MSI), Tumor Microsatellite Instability (MSI)
Test Components
​Only microsatellite instability (MSI) testing is performed.
Useful For

​Evaluation of tumor tissue to identify patients at high risk for having hereditary nonpolyposis colorectal cancer (HNPCC)/Lynch syndrome.

Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
This assay requires submission of 1 cm(2) tumor tissue and normal tissue  for comparison.  
This can be 1 cm(2) in aggregate (eg 5 unstained slides each with 0.2 cm(2) of tumor and normal tissue).
​Tissue (Normal Control) ​Fixed Paraffin Block (marked "Normal Tissue") and 2 H&E Slides ​Slides ​1 slide stained with hematoxylin and eosin and 10 unstained, nonbaked slides 5-micron thick sections of the normal tissue. ​ ​
​Tissue (Tumor) ​Fixed Paraffin Block (marked "Tumor Tissue") and 2 H&E slides ​Slides ​​1 slide stained with hematoxylin and eosin and 10 unstained, nonbaked slides (5-micron thick sections) of the tumor tissue. ​ ​
​Minimum tumor adequacy is approximately 6x6 mm (5000 cells) of tumor, at least 40% tumor nuclei (can be present over multiple slides).  Normal tissue is also required to be present in tissue selected for testing. ​ ​ ​ ​ ​
Collection Processing Instructions

​A Pathology report MUST accompany specimen in order for testing to be performed.

Special Intructions and forms:

Molecular Genetics-Inherited Cancer Syndromes Patient Information Sheet

Specimen Stability Information
Specimen Type Temperature
Varies​ ​ Ambient (preferred)
Frozen
Refrigerated
Rejection Criteria
​Specimens not meeting minimum specimen requirements
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Mayo Medical Laboratories ​Tuesday and Friday ​10 days PCR​
Reference Lab
Mayo Clinic Laboratories
Reference Range Information
Interpretive Report
Interpretation
​The report will include specimen information, assay information, and interpretation of test results. Microsatellite stable (MSS) is reported as MSS/MSI-L (0 or 1 of 5 markers demonstrating instability) or MSI-H (2 or more of 5 markers demonstrating instability).
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​81301 ​ ​ ​Microsatellite Instability gene analysis ​
​88381 ​ ​ ​Microdissection, manual ​
Synonyms/Keywords
DNA Mismatch Repair-related HNPCC, Hereditary Nonpolyposis Colon Cancer (HNPCC), Lynch Syndrome, Microsatellite Instability (MSI), Tumor Microsatellite Instability (MSI)
Test Components
​Only microsatellite instability (MSI) testing is performed.
Ordering Applications
Ordering Application Description
​Centricity ​MSI by PCR
​Cerner None​
​COM ​MSI by PCR
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
This assay requires submission of 1 cm(2) tumor tissue and normal tissue  for comparison.  
This can be 1 cm(2) in aggregate (eg 5 unstained slides each with 0.2 cm(2) of tumor and normal tissue).
​Tissue (Normal Control) ​Fixed Paraffin Block (marked "Normal Tissue") and 2 H&E Slides ​Slides ​1 slide stained with hematoxylin and eosin and 10 unstained, nonbaked slides 5-micron thick sections of the normal tissue. ​ ​
​Tissue (Tumor) ​Fixed Paraffin Block (marked "Tumor Tissue") and 2 H&E slides ​Slides ​​1 slide stained with hematoxylin and eosin and 10 unstained, nonbaked slides (5-micron thick sections) of the tumor tissue. ​ ​
​Minimum tumor adequacy is approximately 6x6 mm (5000 cells) of tumor, at least 40% tumor nuclei (can be present over multiple slides).  Normal tissue is also required to be present in tissue selected for testing. ​ ​ ​ ​ ​
Collection Processing

​A Pathology report MUST accompany specimen in order for testing to be performed.

Special Intructions and forms:

Molecular Genetics-Inherited Cancer Syndromes Patient Information Sheet

Specimen Stability Information
Specimen Type Temperature
Varies​ ​ Ambient (preferred)
Frozen
Refrigerated
Rejection Criteria
​Specimens not meeting minimum specimen requirements
Useful For

​Evaluation of tumor tissue to identify patients at high risk for having hereditary nonpolyposis colorectal cancer (HNPCC)/Lynch syndrome.

Test Components
​Only microsatellite instability (MSI) testing is performed.
Reference Range Information
Interpretive Report
Interpretation
​The report will include specimen information, assay information, and interpretation of test results. Microsatellite stable (MSS) is reported as MSS/MSI-L (0 or 1 of 5 markers demonstrating instability) or MSI-H (2 or more of 5 markers demonstrating instability).
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Mayo Medical Laboratories ​Tuesday and Friday ​10 days PCR​
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​81301 ​ ​ ​Microsatellite Instability gene analysis ​
​88381 ​ ​ ​Microdissection, manual ​
For most current information refer to the Marshfield Laboratory online reference manual.