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25459 Acid Lipase Level

Acid Lipase Level
Test Code: LADLSO
Synonyms/Keywords
​LAL, Lysosomal Acid Lipase, Wolman Disease, Cholesteryl Ester Storage Disease
Useful For
​Lysosomal Acid Lipase (LAL) deficiency is an autosomal recessive lysosomal storage disease caused by a deficiency of LAL, an enzyme responsible for lipid breakdown.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)​​
​No ​Whole Blood ​5 full blood circles of EDTA Lavender Top Tube (LTT) whole blood on specimen collection card ​3 full blood circles on specimen collection card ​ ​ ​
​No ​Whole Blood ​ ​EDTA Lavender Top Tube (LTT) or Sodium Heparin Green Top Tube (GTT) ​2 mL ​2 mL ​
Collection Processing Instructions
​Three fully filled circles are better than five partially filled circles of blood.  Allow blood spots to dry for at least 3 hours.  Do not heat samples or expose to direct sunlight.  Once dry, place in sealed bag with sachet of desiccant.  Ship at room temperature.
Specimen Stability Information
Specimen Type Temperature Time​​
​Dried Blood Spots ​Room Temperature ​1 week
​ ​Refrigerated ​1 month
​ ​Frozen (-20C) ​3 months
​Whole Blood ​Room Temperature ​48 hours
​ ​Refrigerated ​48 hours
Rejection Criteria

Gel Separator Tube

Whole blood >48 hours ​​

Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation​​
​Seattle Children's Laboratory ​Weekly ​7-10 days ​Fluorometric enzyme test for diagnosis of Wolman disease and cholesterol ester storage disease (CESD)
Test Information
​The disease can present in infancy as Wolman disease, or in childhood or adulthood as cholesterol ester storage diseae (CESD).  Wolman disease is a severe phenotype associated with a clinical spectrum that can include growth failure, malabsorption, steatorrhea, hepatosplenomegaly, failure to thrive and hepatic failure.  Later onset disease may present with hyperlipidemia, liver dysfunction, hepatosplenomegaly, liver fibrosis and cirrhosis.  LAL deficiency may present with findings similar to non-alcoholic fatty liver disease (NAFLD), non-alcoholic steatohepatitis (NASH) and alcoholic liver disease.  Dyslipidemia (high cholesterol, triglycerides and/or low HDL) may also be a presenting feature. 
Reference Range Information
Performing Location Reference Range​​
​Seattle Children's Hospital ​40-600 pmol/hr/spot
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments​​
​82657 ​ ​ ​ ​
Synonyms/Keywords
​LAL, Lysosomal Acid Lipase, Wolman Disease, Cholesteryl Ester Storage Disease
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)​​
​No ​Whole Blood ​5 full blood circles of EDTA Lavender Top Tube (LTT) whole blood on specimen collection card ​3 full blood circles on specimen collection card ​ ​ ​
​No ​Whole Blood ​ ​EDTA Lavender Top Tube (LTT) or Sodium Heparin Green Top Tube (GTT) ​2 mL ​2 mL ​
Collection Processing
​Three fully filled circles are better than five partially filled circles of blood.  Allow blood spots to dry for at least 3 hours.  Do not heat samples or expose to direct sunlight.  Once dry, place in sealed bag with sachet of desiccant.  Ship at room temperature.
Specimen Stability Information
Specimen Type Temperature Time​​
​Dried Blood Spots ​Room Temperature ​1 week
​ ​Refrigerated ​1 month
​ ​Frozen (-20C) ​3 months
​Whole Blood ​Room Temperature ​48 hours
​ ​Refrigerated ​48 hours
Rejection Criteria

Gel Separator Tube

Whole blood >48 hours ​​

Useful For
​Lysosomal Acid Lipase (LAL) deficiency is an autosomal recessive lysosomal storage disease caused by a deficiency of LAL, an enzyme responsible for lipid breakdown.
Reference Range Information
Performing Location Reference Range​​
​Seattle Children's Hospital ​40-600 pmol/hr/spot
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation​​
​Seattle Children's Laboratory ​Weekly ​7-10 days ​Fluorometric enzyme test for diagnosis of Wolman disease and cholesterol ester storage disease (CESD)
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments​​
​82657 ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.