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25475 Mycobacterium tuberculosis Complex, Molecular Detection, PCR (MTBRP)

Mycobacterium tuberculosis Complex, Molecular Detection, PCR (MTBRP)
Test Code: MTBRPSO
Synonyms/Keywords
Acid-Fast Bacilli, AFB, Bacillus-Acid Fast, MTB, ​​Mycobacterium tuberculosis, TB, Tuberculosis, Mycobacterium tuberculosis, PCR (MTBRP)
Test Components
Mycobacterium tuberculosis complex DNA unique sequence within the katG gene.​​
Useful For
​Rapid detection of Mycobacterium tuberculosis complex DNA, preferred method. Detection of Mycobacterium tuberculosis, when used in conjunction with mycobacterium culture.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)​​
​No ​Body fluid (body, bone marrow, aspirate, or CSF) ​Steile container ​ ​1.0 mL ​0.5 mL ​
​No ​Gastric washing ​Sterile container ​ ​10.0 mL ​5.0 mL ​
​No ​Respiratory (BAL, bronchial washing or sputum) ​Sterile container ​ ​3.0 mL ​1.5 mL ​
​No ​Stool ​Sterile container ​ ​5-10 grams ​5 grams ​
​No ​Tissue ​Sterile container ​ ​10 mm ​5 mm ​
​No ​Urine ​Sterile container ​ ​2.0 mL ​0.5 mL ​
Collection Processing Instructions

​Body fluid: Only fresh, non-NALC/NaOH-digested body fluid is acceptable.

Gastric washing: Neutralize specimen within 4 hours of collection with 100 mg of sodium carbonate per 5 to 10 mL of gastric washing.

Stool: Only fresh, non-NALC/NaOH-digested stool is acceptable. 

Tissue: Only fresh - keep moist with sterile water or saline, non-NALC/NaOH-digested tissue is acceptable.

Urine: Collect a random urine specimen.

Specimen Stability Information
Specimen Type Temperature Tim​​e
Body Fluid, Gastric, Washing, Respiratory, Stool, Tissue, Urine​ ​Refrigerate (preferred) ​7 days
​ ​Ambient ​7 days
​ ​Frozen ​7 days
Rejection Criteria
​​Blood
​Swab
​Tissue in formalin fluid
​Specimen > 7 days old (with the exception of paraffin tissue)
​Specimen in anaerobe vial or viral transport media (including but not limited to M4, M5, BD viral transport media, thioglycolate broth)
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instru​​mentation
Sent to ​Mayo Medical Laboratories ​Monday through Sunday ​1 day ​Real-Time Polymerase Chain Reaction (PCR)
Reference Lab
Mayo Clinic Laboratories
Test Information
This test should always be performed in conjunction with mycobacterial culture. This rapid PCR assay detects Mycobacterium tuberculosis complex nucleic acid and, therefore, does not distinguish between viable, disease-related organisms and nucleic acid persisting from prior infection. Test results should be correlated with patient symptoms and clinical presentation before a definitive diagnosis is made.
Reference Range Information
​See Report
Interpretation
A positive result indicates the presence of Mycobacterium tuberculosis complex DNA. Members of the Mycobacterium tuberculosis complex detected by this assay include Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium bovis Bacillus Calmette-Guerin, Mycobacterium africanum, Mycobacterium canetti, and Mycobacterium microti. The other species within the Mycobacterium tuberculosis complex (Mycobacterium bovis subspecies caprae and Mycobacterium pinnepedi) should, in theory, be detected using the primer and probe sequences in this assay, but they have not been tested at this time. This assay method does not distinguish between the species of the Mycobacterium tuberculosis complex.
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comm​​ents
87556​ ​ ​1 ​M. tuberculosis Complex PCR ​
​87015 ​ ​1 ​Mycobacteria Culture, Conc ​If needed (Reflex)
Synonyms/Keywords
Acid-Fast Bacilli, AFB, Bacillus-Acid Fast, MTB, ​​Mycobacterium tuberculosis, TB, Tuberculosis, Mycobacterium tuberculosis, PCR (MTBRP)
Test Components
Mycobacterium tuberculosis complex DNA unique sequence within the katG gene.​​
Ordering Applications
Ordering Application Descri​​ption
​Clinical Order Manager ​M. Tuberculosis, PCR
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)​​
​No ​Body fluid (body, bone marrow, aspirate, or CSF) ​Steile container ​ ​1.0 mL ​0.5 mL ​
​No ​Gastric washing ​Sterile container ​ ​10.0 mL ​5.0 mL ​
​No ​Respiratory (BAL, bronchial washing or sputum) ​Sterile container ​ ​3.0 mL ​1.5 mL ​
​No ​Stool ​Sterile container ​ ​5-10 grams ​5 grams ​
​No ​Tissue ​Sterile container ​ ​10 mm ​5 mm ​
​No ​Urine ​Sterile container ​ ​2.0 mL ​0.5 mL ​
Collection Processing

​Body fluid: Only fresh, non-NALC/NaOH-digested body fluid is acceptable.

Gastric washing: Neutralize specimen within 4 hours of collection with 100 mg of sodium carbonate per 5 to 10 mL of gastric washing.

Stool: Only fresh, non-NALC/NaOH-digested stool is acceptable. 

Tissue: Only fresh - keep moist with sterile water or saline, non-NALC/NaOH-digested tissue is acceptable.

Urine: Collect a random urine specimen.

Specimen Stability Information
Specimen Type Temperature Tim​​e
Body Fluid, Gastric, Washing, Respiratory, Stool, Tissue, Urine​ ​Refrigerate (preferred) ​7 days
​ ​Ambient ​7 days
​ ​Frozen ​7 days
Rejection Criteria
​​Blood
​Swab
​Tissue in formalin fluid
​Specimen > 7 days old (with the exception of paraffin tissue)
​Specimen in anaerobe vial or viral transport media (including but not limited to M4, M5, BD viral transport media, thioglycolate broth)
Useful For
​Rapid detection of Mycobacterium tuberculosis complex DNA, preferred method. Detection of Mycobacterium tuberculosis, when used in conjunction with mycobacterium culture.
Test Components
Mycobacterium tuberculosis complex DNA unique sequence within the katG gene.​​
Reference Range Information
​See Report
Interpretation
A positive result indicates the presence of Mycobacterium tuberculosis complex DNA. Members of the Mycobacterium tuberculosis complex detected by this assay include Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium bovis Bacillus Calmette-Guerin, Mycobacterium africanum, Mycobacterium canetti, and Mycobacterium microti. The other species within the Mycobacterium tuberculosis complex (Mycobacterium bovis subspecies caprae and Mycobacterium pinnepedi) should, in theory, be detected using the primer and probe sequences in this assay, but they have not been tested at this time. This assay method does not distinguish between the species of the Mycobacterium tuberculosis complex.
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Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instru​​mentation
Sent to ​Mayo Medical Laboratories ​Monday through Sunday ​1 day ​Real-Time Polymerase Chain Reaction (PCR)
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comm​​ents
87556​ ​ ​1 ​M. tuberculosis Complex PCR ​
​87015 ​ ​1 ​Mycobacteria Culture, Conc ​If needed (Reflex)
For most current information refer to the Marshfield Laboratory online reference manual.