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25500 HIV-1 RNA Detect/Quant, P (HIVQN)

HIV-1 RNA Detect/Quant, P (HIVQN)
Test Code: HIVDQSO
Synonyms/Keywords
Acquired Immune Deficiency Syndrome (AIDS), Human Immunodeficiency Virus PCR, Viral Load, HIV-1 PCR
Test Components
​HIV-1 RNA Detection & Quantitation 
HIV-1 Genotypic PR-RT (Test code HIVPR) if needed
Useful For

Diagnosis of HIV-1 infection in individuals with acute or early HIV-1 infection.

Diagnosis of HIV-1 infection in infants of <18 months of age born to HIV-1 infected mothers.

Quantifying plasma HIV-1 RNA levels (viral load) in HIV-1 infected individuals:
 -Before initiating anti-HIV drug therapy (baseline viral load)
 -Who may have developed HIV-1 drug resistance while on anti-HIV-1 therapy 
 -Who may be noncompliant with anti-HIV-1 therapy

Monitoring HIV-1 disease progression while on or off antiretroviral drug therapy

Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)​​
No​ ​EDTA Plasma EDTA Pink Top Tube (PTT) ​​EDTA Lavender Top Tube (LTT) ​2.5 mL ​1.2 mL ​
Collection Processing Instructions
Spin down and remove plasma from cells within 6 hours of draw, place in a plastic vial. Freeze specimen immediately and ship on dry ice. Specimen is stable refrigerated for up to 5 days, if shipment is delayed for > 5 days, freeze plasma at -70 degrees C (up to 35 days) until shipment on dry ice.
Specimen Stability Information
Specimen Type Temperature Time​​
Plasma​ ​ ​Frozen (Preferred) ​35 days
​Refrigerate ​5 days
Rejection Criteria
​Green Top Tube (Heparin)
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrument​​ation
​Mayo Medical Laboratories ​Monday through Saturday 1-3 days ​Real-time reverse transcription-polymerase chain reaction (RT-PCR)
Reference Lab
Mayo Clinic Laboratories
Test Information
​​Mayo test HIV-1 Genotyping PR-RT, Resistance (HIVPR) may be reflexed from a positive result.
Reference Range Information
Undetected.  See Report.
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comm​​ents
87536 ​ ​ ​ ​
Synonyms/Keywords
Acquired Immune Deficiency Syndrome (AIDS), Human Immunodeficiency Virus PCR, Viral Load, HIV-1 PCR
Test Components
​HIV-1 RNA Detection & Quantitation 
HIV-1 Genotypic PR-RT (Test code HIVPR) if needed
Ordering Applications
Ordering Application Description
​Centricity ​HIV-1 RNA Detect/Quant, P (HIVQN)
​Cerner ​HIV-1 RNA Detect/Quant, P (HIVQN)
​COM ​HIV-1 RNA Detect/Quant, P (HIVQN)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)​​
No​ ​EDTA Plasma EDTA Pink Top Tube (PTT) ​​EDTA Lavender Top Tube (LTT) ​2.5 mL ​1.2 mL ​
Collection Processing
Spin down and remove plasma from cells within 6 hours of draw, place in a plastic vial. Freeze specimen immediately and ship on dry ice. Specimen is stable refrigerated for up to 5 days, if shipment is delayed for > 5 days, freeze plasma at -70 degrees C (up to 35 days) until shipment on dry ice.
Specimen Stability Information
Specimen Type Temperature Time​​
Plasma​ ​ ​Frozen (Preferred) ​35 days
​Refrigerate ​5 days
Rejection Criteria
​Green Top Tube (Heparin)
Useful For

Diagnosis of HIV-1 infection in individuals with acute or early HIV-1 infection.

Diagnosis of HIV-1 infection in infants of <18 months of age born to HIV-1 infected mothers.

Quantifying plasma HIV-1 RNA levels (viral load) in HIV-1 infected individuals:
 -Before initiating anti-HIV drug therapy (baseline viral load)
 -Who may have developed HIV-1 drug resistance while on anti-HIV-1 therapy 
 -Who may be noncompliant with anti-HIV-1 therapy

Monitoring HIV-1 disease progression while on or off antiretroviral drug therapy

Test Components
​HIV-1 RNA Detection & Quantitation 
HIV-1 Genotypic PR-RT (Test code HIVPR) if needed
Reference Range Information
Undetected.  See Report.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrument​​ation
​Mayo Medical Laboratories ​Monday through Saturday 1-3 days ​Real-time reverse transcription-polymerase chain reaction (RT-PCR)
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comm​​ents
87536 ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.