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25506 Immunoglobulins G-A-M & Immunotyping Add-On

Immunoglobulins G-A-M & Immunotyping Add-On
Test Code: GAM-IT
Synonyms/Keywords
Immunoglobulins, quantitative; GAM; IgG-A-M + immunotyping​, GAMIT
Test Components
IgG, IgA and IgM + Immunotyping (if required by interpreter).  There is an additional charge if Immunotyping is required​.
Useful For
Quantitative immunoglobulins are useful for detecting or monitoring of monoclonal gammopathies and immune deficiencies.  Immunotyping of serum is useful in determining monoclonal gammopathies.  Immunoglobulin quantitation is used to monitor the size of a monoclonal protein that is contained in a background of polyclonal immunoglobulins; however, changes in the immunoglobulin quantitation may reflect changes in the background immunoglobulins.​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​ Serum​ Red Top Tube (RTT)​ Serum Separator Tube (SST)​ 2.0 mL​ 1.5 mL serum​ 1.0 mL serum​
Collection Processing Instructions
This is an add-on test for quantitative immunoglobulins when the lab already has blood and the clinician wishes to have a complete protein workup involving quantitative immunoglobulins and Immunotyping. Test order further authorizes the lab to phenotype heavy and light chains (kappa and lambda) using Immunotyping electrophoresis (IT) based on PhD/Pathologist interpretation of previously obtained protein electrophoresis pattern (PEP) and quantitative immunoglobulin results. If Immunotyping electrophoresis (IT) is required, an additional charge will be generated. If the ordering physician wishes to obtain Immunotyping electrophoresis (IT) regardless of the quantitative immunoglobulin levels, add comment to order: "Do IF regardless".  Immunotyping should not be done routinely to follow known monoclonal patients.​
Specimen Stability Information
Specimen Type Temperature Time
Serum​ Refrigerate​ 2 weeks​
Rejection Criteria
Plasma samples
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ 2 days​

Turbidimetric method/The Binding Site Optilite analyzer

Immunotyping Electrophoresis/Sebia Capillarys 2 Protein System​

Reference Range Information
Performing Location Reference Range
Marshfield​

See table Serum Immunoglobulin Reference Ranges for IgG, IgA and IgM

Interpretation of Immunotyping is determined by interpreter​

Interpretation
Increased serum immunoglobulin concentrations occur due to polyclonal or oligoclonal immunoglobulin proliferation in hepatic disease (hepatitis, liver cirrhosis), connective tissue diseases, acute and chronic infections, as well as in the cord blood of neonates with intra uterine and perinatal infections. Elevations of IgG, IgA, or IgM may occur in monoclonal gammopathies such as multiple myeloma (IgG, IgA), macroglobulinemia (IgM), primary systemic amyloidosis, monoclonal gammopathy of undetermined significance (MGUS), and related disorders.  Decreased levels are found in patients with primary or secondary immune deficiencies.
 
Immunotypings are done to determine monoclonal specificity, or to rule out a monoclonal gammopathy.  Monoclonal gammopathies are characterized by an uncontrolled proliferation of a single clone of plasma cells at the expense of other clones.  This often leads to the synthesis of large amounts of one immunoglobulin with decreased levels of normal immunoglobulins.  Plasma cell myeloma, along with decreased levels of normal immunoglobulins can lead to life threatening consequences.  Light chain disease, where only kappa and lambda monoclonal light chains are synthesized, may also lead to suppression of normal immunoglobulins.  These low molecular weight immunoglobulin fragments are filtered through the glomerulus of the kidney and into the urine, giving a serum electrophoresis pattern showing hypogammaglobulinemia, with a faint monoclonal band or no band at all in the serum.​
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
82784​ 3​ IgG, IgA and IgM​

86334​ Immunotyping (if required)​
Synonyms/Keywords
Immunoglobulins, quantitative; GAM; IgG-A-M + immunotyping​, GAMIT
Test Components
IgG, IgA and IgM + Immunotyping (if required by interpreter).  There is an additional charge if Immunotyping is required​.
Ordering Applications
Ordering Application Description
​Centricity ​Prot. Imm. Type GAM Add-On
​Cerner ​Prot.-GAM/IF Add-On
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​ Serum​ Red Top Tube (RTT)​ Serum Separator Tube (SST)​ 2.0 mL​ 1.5 mL serum​ 1.0 mL serum​
Collection Processing
This is an add-on test for quantitative immunoglobulins when the lab already has blood and the clinician wishes to have a complete protein workup involving quantitative immunoglobulins and Immunotyping. Test order further authorizes the lab to phenotype heavy and light chains (kappa and lambda) using Immunotyping electrophoresis (IT) based on PhD/Pathologist interpretation of previously obtained protein electrophoresis pattern (PEP) and quantitative immunoglobulin results. If Immunotyping electrophoresis (IT) is required, an additional charge will be generated. If the ordering physician wishes to obtain Immunotyping electrophoresis (IT) regardless of the quantitative immunoglobulin levels, add comment to order: "Do IF regardless".  Immunotyping should not be done routinely to follow known monoclonal patients.​
Specimen Stability Information
Specimen Type Temperature Time
Serum​ Refrigerate​ 2 weeks​
Rejection Criteria
Plasma samples
Useful For
Quantitative immunoglobulins are useful for detecting or monitoring of monoclonal gammopathies and immune deficiencies.  Immunotyping of serum is useful in determining monoclonal gammopathies.  Immunoglobulin quantitation is used to monitor the size of a monoclonal protein that is contained in a background of polyclonal immunoglobulins; however, changes in the immunoglobulin quantitation may reflect changes in the background immunoglobulins.​
Test Components
IgG, IgA and IgM + Immunotyping (if required by interpreter).  There is an additional charge if Immunotyping is required​.
Reference Range Information
Performing Location Reference Range
Marshfield​

See table Serum Immunoglobulin Reference Ranges for IgG, IgA and IgM

Interpretation of Immunotyping is determined by interpreter​

Interpretation
Increased serum immunoglobulin concentrations occur due to polyclonal or oligoclonal immunoglobulin proliferation in hepatic disease (hepatitis, liver cirrhosis), connective tissue diseases, acute and chronic infections, as well as in the cord blood of neonates with intra uterine and perinatal infections. Elevations of IgG, IgA, or IgM may occur in monoclonal gammopathies such as multiple myeloma (IgG, IgA), macroglobulinemia (IgM), primary systemic amyloidosis, monoclonal gammopathy of undetermined significance (MGUS), and related disorders.  Decreased levels are found in patients with primary or secondary immune deficiencies.
 
Immunotypings are done to determine monoclonal specificity, or to rule out a monoclonal gammopathy.  Monoclonal gammopathies are characterized by an uncontrolled proliferation of a single clone of plasma cells at the expense of other clones.  This often leads to the synthesis of large amounts of one immunoglobulin with decreased levels of normal immunoglobulins.  Plasma cell myeloma, along with decreased levels of normal immunoglobulins can lead to life threatening consequences.  Light chain disease, where only kappa and lambda monoclonal light chains are synthesized, may also lead to suppression of normal immunoglobulins.  These low molecular weight immunoglobulin fragments are filtered through the glomerulus of the kidney and into the urine, giving a serum electrophoresis pattern showing hypogammaglobulinemia, with a faint monoclonal band or no band at all in the serum.​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ 2 days​

Turbidimetric method/The Binding Site Optilite analyzer

Immunotyping Electrophoresis/Sebia Capillarys 2 Protein System​

For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
82784​ 3​ IgG, IgA and IgM​

86334​ Immunotyping (if required)​
For most current information refer to the Marshfield Laboratory online reference manual.