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25542 Plasma Hemoglobin (PLHBB)

Plasma Hemoglobin (PLHBB)
Test Code: PLHBBSO
Synonyms/Keywords
​Plasma Free Hemoglobin
Useful For

Determining whether hemolysis is occurring such as from:

-Transfusion reaction

-Mechanical fragmentation of red blood cells

-Relative comparison to baseline levels in extracorporeal membrane oxygenation (ECMO) and centrifugal ventricular assist device (cVAD) patients to assess pump disruption

Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
​No ​Plasma ​EDTA Lavender Top Tube (LTT) ​ ​2 mL ​1.5 mL
Collection Processing Instructions
Spin down and transfer plasma to a secondary aliquot tube within 2 hours of draw.
Specimen Stability Information
Specimen Type Temperature Time
​Plasma ​ ​ ​Refrigerate (preferred) ​7 days
​Frozen ​7 days
​Ambient ​4 days
Rejection Criteria
Serum
Interference
​Falsely elevated levels result if left > 2 hours due to artefactual post draw RBC lysis.
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Medical Laboratories ​Monday through Sunday ​1 day ​Spectrophotometry (SP)
Reference Lab
Mayo Clinic Laboratories
Reference Range Information

TOTAL HEMOGLOBIN > or =12 months: 0.0-15.2 mg/dL
Reference values have not been established for patients who are <12 months of age.

OXYHEMOGLOBIN > or =12 months: 0.0-12.4 mg/dL
Reference values have not been established for patients who are <12 months of age.

Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​83051 ​ ​ ​ ​
Synonyms/Keywords
​Plasma Free Hemoglobin
Ordering Applications
Ordering Application Description
​Centricity ​Hemoglobin, Plasma
​Cerner ​Hemoglobin, Plasma
​Clinical Order Manager ​Hemoglobin, Plasma
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
​No ​Plasma ​EDTA Lavender Top Tube (LTT) ​ ​2 mL ​1.5 mL
Collection Processing
Spin down and transfer plasma to a secondary aliquot tube within 2 hours of draw.
Specimen Stability Information
Specimen Type Temperature Time
​Plasma ​ ​ ​Refrigerate (preferred) ​7 days
​Frozen ​7 days
​Ambient ​4 days
Rejection Criteria
Serum
Interference
​Falsely elevated levels result if left > 2 hours due to artefactual post draw RBC lysis.
Useful For

Determining whether hemolysis is occurring such as from:

-Transfusion reaction

-Mechanical fragmentation of red blood cells

-Relative comparison to baseline levels in extracorporeal membrane oxygenation (ECMO) and centrifugal ventricular assist device (cVAD) patients to assess pump disruption

Reference Range Information

TOTAL HEMOGLOBIN > or =12 months: 0.0-15.2 mg/dL
Reference values have not been established for patients who are <12 months of age.

OXYHEMOGLOBIN > or =12 months: 0.0-12.4 mg/dL
Reference values have not been established for patients who are <12 months of age.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Medical Laboratories ​Monday through Sunday ​1 day ​Spectrophotometry (SP)
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​83051 ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.