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25601 Plasma Cell Proliferative FISH, Tissue (PLASF)

Plasma Cell Proliferative FISH, Tissue (PLASF)
Test Code: PLASFSO
Synonyms/Keywords

-13/13q-, RB1/LAMP1
t(11;14) CCND1/IGH
14q32 rearrangement, IGH
17p-, TP53/D17Z1
+3/+7, D3Z1/D7Z1
+9/+15, D9Z1/D15Z4
1q gain, TP73/CKS1B
8q24.1 rearrangement, MYC

When an IGH rearrangement is identified, reflex testing is performed to identify the translocation partner. Probes include identification of t(4;14)(p16.3;q32) FGFR3/IGH, t(14;16)(q32;q23) IGH/MAF, t(14;20)(q32;q12), IGH/MAFB or t(6;14)(p21;q32) CCND3/IGH.

This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.

Indicate if the entire panel is to be performed. If the patient is being tracked for known abnormalities, indicate which probes should be used.

Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
​No ​Tissue ​Tissue Block ​Slides ​1 block/2 slides ​1 block/2 slides
Collection Processing Instructions
Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used. For each probe set ordered, 2 consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin (H&E) stained slide.
Specimen Stability Information
Specimen Type Temperature
​Tissue ​ ​Ambient (preferred)
​Refrigerated
Rejection Criteria
No specimen should be rejected. If specimen not received at appropriate temperature, include note to laboratory. Contact the laboratory with questions.
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Medical Laboratories ​Monday through Sunday ​7 days ​This test is performed using both commercially available and laboratory-developed probes. Deletion or monosomy of chromosomes 13 and 17 and copy number gain of 1q are detected using enumeration strategy probes. Centromere probes are used to detect chromosomal aneusomy of chromosomes 3, 7, 9, and 15. Translocations involving IGH with FGFR3, CCND1, CCND3, MAF, and MAFB are detected using dual-color, dual-fusion (D-FISH) strategy probes. Rearrangement of MYC is detected using a break-apart strategy (BAP) probe. Formalin-fixed, paraffin-embedded tissues are cut at 5 microns and mounted on positively charged glass slides. The selection of tissue and the identification of target areas on the hematoxylin and eosin (H and E)-stained slide are performed by a pathologist. Using the H and E-stained slide as a reference, target areas are etched with a diamond-tipped etcher on the back of the unstained slide to be assayed.  Each probe set is hybridized to the appropriate target areas and 2 technologists analyze 50 interphase nuclei each (100 total) with the results expressed as the percent abnormal nuclei.(Unpublished Mayo method)
Reference Lab
Mayo Clinic Laboratories
Reference Range Information
​Interpretive Report
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​88291 ​ ​1 ​Report ​
​88271 ​ ​2 ​Cytogenetic DNA probe ​
​88271 ​ ​varies ​DNA probes ​As needed
Synonyms/Keywords

-13/13q-, RB1/LAMP1
t(11;14) CCND1/IGH
14q32 rearrangement, IGH
17p-, TP53/D17Z1
+3/+7, D3Z1/D7Z1
+9/+15, D9Z1/D15Z4
1q gain, TP73/CKS1B
8q24.1 rearrangement, MYC

When an IGH rearrangement is identified, reflex testing is performed to identify the translocation partner. Probes include identification of t(4;14)(p16.3;q32) FGFR3/IGH, t(14;16)(q32;q23) IGH/MAF, t(14;20)(q32;q12), IGH/MAFB or t(6;14)(p21;q32) CCND3/IGH.

This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.

Indicate if the entire panel is to be performed. If the patient is being tracked for known abnormalities, indicate which probes should be used.

Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
​No ​Tissue ​Tissue Block ​Slides ​1 block/2 slides ​1 block/2 slides
Collection Processing
Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used. For each probe set ordered, 2 consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin (H&E) stained slide.
Specimen Stability Information
Specimen Type Temperature
​Tissue ​ ​Ambient (preferred)
​Refrigerated
Rejection Criteria
No specimen should be rejected. If specimen not received at appropriate temperature, include note to laboratory. Contact the laboratory with questions.
Reference Range Information
​Interpretive Report
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Medical Laboratories ​Monday through Sunday ​7 days ​This test is performed using both commercially available and laboratory-developed probes. Deletion or monosomy of chromosomes 13 and 17 and copy number gain of 1q are detected using enumeration strategy probes. Centromere probes are used to detect chromosomal aneusomy of chromosomes 3, 7, 9, and 15. Translocations involving IGH with FGFR3, CCND1, CCND3, MAF, and MAFB are detected using dual-color, dual-fusion (D-FISH) strategy probes. Rearrangement of MYC is detected using a break-apart strategy (BAP) probe. Formalin-fixed, paraffin-embedded tissues are cut at 5 microns and mounted on positively charged glass slides. The selection of tissue and the identification of target areas on the hematoxylin and eosin (H and E)-stained slide are performed by a pathologist. Using the H and E-stained slide as a reference, target areas are etched with a diamond-tipped etcher on the back of the unstained slide to be assayed.  Each probe set is hybridized to the appropriate target areas and 2 technologists analyze 50 interphase nuclei each (100 total) with the results expressed as the percent abnormal nuclei.(Unpublished Mayo method)
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​88291 ​ ​1 ​Report ​
​88271 ​ ​2 ​Cytogenetic DNA probe ​
​88271 ​ ​varies ​DNA probes ​As needed
For most current information refer to the Marshfield Laboratory online reference manual.