Skip Ribbon Commands
Skip to main content
Sign In
Navigate Up

25628 B-Cell Lymphoma Tissue, FISH (BLYM)

B-Cell Lymphoma Tissue, FISH (BLYM)
Test Code: BLYMFSO
Synonyms/Keywords
​BCL2 (18q21) rearrangement
BCL6 (3q27) rearrangement
Burkitt lymphoma
CCND2
Diffuse Large Cell Lymphoma/"Double Hit"
Follicular lymphoma
MALT (18q21) rearrangement
MALT lymphoma
Mantle Cell Lymphoma (MCL)
MYC (8q24.1) rearrangement
MYC/Kappa
MYC/Lambda
Splenic Marginal Zone Lymphoma
t(11;14) (q13;q32) - CCND1/IGH
t(11;18) (q21;q21) - BIRC3/MALT1
t(14;18) (q32;q21) - IGH/MALT1
t(14;18)(q32;q21) - IGH/BCL2
t(2;8)(p12;q24) - IGK/MYC
t(8;14) (q24.1;q32) - MYC/IGH
t(8;22) (q24.1;q11.2) - MYC/IGL
Burkitt-like lymphoma
Test Components
​This test does not include a pathology consultation. If a pathology consultation is requested, 70012 / Pathology Consultation should be ordered and the appropriate FISH test will be ordered and performed at an additional charge. Mayo Hematopathology Consultants are involved in both the pre-analytic (tissue adequacy and probe selection, when applicable) and post-analytic (interpretation of FISH results in context of specific case, when applicable) phases.
 
This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.
 
Depending on the lymphoma subtype suspected, the most appropriate probes to order are listed in the table: Common Chromosome Abnormalities in B-cell Lymphomas in Clinical Information.
 
If the patient is being tracked for known abnormalities, indicate which probes should be used.
Useful For
​Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with various B-cell lymphomas
 
Tracking known chromosome abnormalities and response to therapy in patients with B-cell lymphomas
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​Lymph node ​Tissue block ​Slides ​ ​ ​
​Solid tumor ​Tissue block ​Slides ​ ​ ​
Collection Processing Instructions

​Tissue Block: Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used.

Slides:  For each probe set ordered, 2 consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.

Provide a reason for referral with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.

Specimen Stability Information
Specimen Type Temperature
​Tissue ​Room Temperature (preferred)
​ ​Refrigerate
Rejection Criteria
No specimen should be rejected
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Medical Laboratories ​Monday through Sunday ​4-7 days ​Fluorescence In Situ Hybridization (FISH)
Reference Lab
Mayo Clinic Laboratories
Test Information

This test was developed and its performance characteristics determined by Marshfield Labs.  It has not been cleared or approved by the U.S. Food and Drug Administration.  The FDA has determined that such clearance or approval is not necessary.  This test is for clinical purposes.  It should not be regarded as investigational or for research.  This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity clinical testing.

​
Interpretation
​A neoplastic clone is detected when the percent of cells with an abnormality exceeds the normal reference range for any given probe.
 
Detection of an abnormal clone is supportive of a diagnosis of a B-cell lymphoma. The specific abnormality detected may help subtype the neoplasm.
 
The absence of an abnormal clone does not rule out the presence of a neoplastic disorder.
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​88291 ​ ​1 ​ ​
​88271 ​ ​2 ​ ​
​88271 ​ ​2 ​Probe, +2 ​If needed
​88271 ​ ​1 ​Probe, +1 ​​If needed
​88271 ​ ​2 ​Probe, +2 ​​If needed
​88271 ​ ​3 ​Probe, +3 ​​If needed
​88274 ​ ​1 ​Interphases, <25 ​​If needed
​88274 ​ ​1 ​Interphases, 25-99 ​​If needed
​88275 ​ ​1 ​Interphases, >=100 ​​If needed
Classification

This test was developed and its performance characteristics determined by Marshfield Labs.  It has not been cleared or approved by the U.S. Food and Drug Administration.  The FDA has determined that such clearance or approval is not necessary.  This test is for clinical purposes.  It should not be regarded as investigational or for research.  This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity clinical testing.

​
Synonyms/Keywords
​BCL2 (18q21) rearrangement
BCL6 (3q27) rearrangement
Burkitt lymphoma
CCND2
Diffuse Large Cell Lymphoma/"Double Hit"
Follicular lymphoma
MALT (18q21) rearrangement
MALT lymphoma
Mantle Cell Lymphoma (MCL)
MYC (8q24.1) rearrangement
MYC/Kappa
MYC/Lambda
Splenic Marginal Zone Lymphoma
t(11;14) (q13;q32) - CCND1/IGH
t(11;18) (q21;q21) - BIRC3/MALT1
t(14;18) (q32;q21) - IGH/MALT1
t(14;18)(q32;q21) - IGH/BCL2
t(2;8)(p12;q24) - IGK/MYC
t(8;14) (q24.1;q32) - MYC/IGH
t(8;22) (q24.1;q11.2) - MYC/IGL
Burkitt-like lymphoma
Test Components
​This test does not include a pathology consultation. If a pathology consultation is requested, 70012 / Pathology Consultation should be ordered and the appropriate FISH test will be ordered and performed at an additional charge. Mayo Hematopathology Consultants are involved in both the pre-analytic (tissue adequacy and probe selection, when applicable) and post-analytic (interpretation of FISH results in context of specific case, when applicable) phases.
 
This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.
 
Depending on the lymphoma subtype suspected, the most appropriate probes to order are listed in the table: Common Chromosome Abnormalities in B-cell Lymphomas in Clinical Information.
 
If the patient is being tracked for known abnormalities, indicate which probes should be used.
Ordering Applications
Ordering Application Description
​COM ​B-Cell Lymphoma Fish, Tissue
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​Lymph node ​Tissue block ​Slides ​ ​ ​
​Solid tumor ​Tissue block ​Slides ​ ​ ​
Collection Processing

​Tissue Block: Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used.

Slides:  For each probe set ordered, 2 consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.

Provide a reason for referral with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.

Specimen Stability Information
Specimen Type Temperature
​Tissue ​Room Temperature (preferred)
​ ​Refrigerate
Rejection Criteria
No specimen should be rejected
Useful For
​Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with various B-cell lymphomas
 
Tracking known chromosome abnormalities and response to therapy in patients with B-cell lymphomas
Test Components
​This test does not include a pathology consultation. If a pathology consultation is requested, 70012 / Pathology Consultation should be ordered and the appropriate FISH test will be ordered and performed at an additional charge. Mayo Hematopathology Consultants are involved in both the pre-analytic (tissue adequacy and probe selection, when applicable) and post-analytic (interpretation of FISH results in context of specific case, when applicable) phases.
 
This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.
 
Depending on the lymphoma subtype suspected, the most appropriate probes to order are listed in the table: Common Chromosome Abnormalities in B-cell Lymphomas in Clinical Information.
 
If the patient is being tracked for known abnormalities, indicate which probes should be used.
Interpretation
​A neoplastic clone is detected when the percent of cells with an abnormality exceeds the normal reference range for any given probe.
 
Detection of an abnormal clone is supportive of a diagnosis of a B-cell lymphoma. The specific abnormality detected may help subtype the neoplasm.
 
The absence of an abnormal clone does not rule out the presence of a neoplastic disorder.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Medical Laboratories ​Monday through Sunday ​4-7 days ​Fluorescence In Situ Hybridization (FISH)
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​88291 ​ ​1 ​ ​
​88271 ​ ​2 ​ ​
​88271 ​ ​2 ​Probe, +2 ​If needed
​88271 ​ ​1 ​Probe, +1 ​​If needed
​88271 ​ ​2 ​Probe, +2 ​​If needed
​88271 ​ ​3 ​Probe, +3 ​​If needed
​88274 ​ ​1 ​Interphases, <25 ​​If needed
​88274 ​ ​1 ​Interphases, 25-99 ​​If needed
​88275 ​ ​1 ​Interphases, >=100 ​​If needed
Classification

This test was developed and its performance characteristics determined by Marshfield Labs.  It has not been cleared or approved by the U.S. Food and Drug Administration.  The FDA has determined that such clearance or approval is not necessary.  This test is for clinical purposes.  It should not be regarded as investigational or for research.  This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity clinical testing.

​
For most current information refer to the Marshfield Laboratory online reference manual.