Bacterial Culture, Cystic Fibrosis with Antimicrobial Susceptibilities (CFRCS)

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Marshfield Clinic Public WebSite

Test Code: CFRCSO

Overview
Ordering
Specimen
Performing
Clinical/Interpretive
Contacts
Coding

Useful For

Detection of aerobic bacterial pathogens in specimens from patients with cystic fibrosis

Determining the in vitro antimicrobial susceptibility of potentially pathogenic aerobic bacteria, if appropriate

NOTE - Not to be ordered on Outreach clients.

Specimen Requirements

Submit only 1 of the following specimens:

Specimen Type	Preferred Container/Tube	Acceptable Container/Tube	Specimen Volume	Specimen Minimum Volume (allows for 1 repeat)
Sputum, expectorated or induced	Sterile, screw-capped container		2 mL	2 mL
Tracheal endotrach secretions, Bronchial wash or lavage	Sterile, screw-capped container		2 mL	2 mL
Throat	ESwab	Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium	One swab

Collection Processing Instructions

Specimens should be delivered to the laboratory as soon as possible. When culture is delayed, successful isolation of bacterial pathogens is less likely, due to the overgrowth of usual oropharyngeal flora.

Shipping Instructions: Specimen must arrive within 48 hours of collection.

Specimen Stability Information

Specimen Type	Temperature	Time
All Specimens	Refrigerate	48 hours

Rejection Criteria

Specimen >48 hours old
Pooled 24 hour samples
Dry swab

Performing Laboratory Information

Performing Location	Day(s) Test Performed	Analytical Time	Methodology/Instrumentation
Mayo Medical Laboratories	Monday through Sunday	5-12 days	Conventional Culture Technique

Reference Lab

Mayo Clinic Laboratories

Test Information

Life expectancy of patients with CF has increased steadily over the past 50 years, in large part due to improvements in the management of lung disease in this patient population. Still, chronic lung infection is responsible for 75% to 85% of deaths in patients with CF. Appropriate treatment for the causative organism can reduce morbidity and mortality.

The number of microbial species associated with CF lung disease is relatively limited. These include Pseudomonas aeruginosa (mucoid and nonmucoid), Staphylococcus aureus, Burkholderia cepacia complex, Stenotrophomonas maltophilia, other nonfermenting gram-negative rods, Haemophilus influenzae, and Streptococcus pneumoniae. Nontuberculous mycobacteria, common respiratory viruses and Aspergillus species may also play a role in CF lung disease. This culture, which is specifically designed for CF patients, utilizes conventional and additional selective media (compared to non-CF respiratory cultures) to isolate bacteria commonly associated with pulmonary disease in CF patients.

Reference Range Information

Performing Location	Reference Range
Mayo Medical Laboratories	No growth or usual flora Identification of probable pathogens

Performing Location

Reference Range

Mayo Medical Laboratories

No growth or usual flora

Identification of probable pathogens

Interpretation

A negative test result is no growth of bacteria or growth of only usual flora. A negative result does not rule out all causes of infectious lung disease.

Organisms associated with lower respiratory tract infections are reported.

For positive test results, pathogenic bacteria are identified. CF patients may be colonized or chronically infected by some organisms over a long period of time, therefore, positive results must be interpreted in conjunction with previous findings and the clinical picture to appropriately evaluate results

Ordering Applications

Ordering Application	Description
Centricity	Culture, Cystic Fibrosis w/susceptibility (CFRC)
Cerner	Culture, Cystic Fibrosis w/susceptibility (CFRC)
COM	Culture, Cystic Fibrosis w/susceptibility (CFRC)

If the ordering application you are looking for is not listed, contact your local laboratory for assistance.