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22187 Antithrombin III Antigen, Immunologic (ATTI)

Antithrombin III Antigen, Immunologic (ATTI)
Test Code: MISC
Synonyms/Keywords
​Ref Lab Code: 9031,
Antithrombin III, Immunologic, Plasma, AT III Antigen/Immunologic, AT3 Antigen/Immunologic
Useful For
​Assessing abnormal results of the antithrombin activity assay. (ATTF/9030 Antithrombin Activity, Plasma), which is recommended as the primary (screening) antithrombin assay
 
Diagnosing antithrombin deficiency, acquired or congenital, in conjunction with measurement of antithrombin activity
 
As an adjunct in the diagnosis and management of carbohydrate-deficient glycoprotein syndromes
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Plasma​ Citrated Blue Top Tube (BTT)​ ​ 1 mL​ 0.5 mL​ ​
Collection Processing Instructions

​Submit sample (platelet poor plasma) in a plastic vial.

1. Spin down, remove plasma, and spin plasma again.
2. Freeze specimen immediately at < or =-40 degrees C, if possible.
Additional Information:
1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.
2. Each coagulation assay requested should have its own vial.
3. Heparin treatment may lower plasma antithrombin.
4. Coagulation testing is highly complex, often requiring the performance of multiple assays and correlation with clinical information. For that reason, we suggest ordering THRMP/83093 Coagulation Consultation, Thrombosis/Hypercoagulability, Blood and Plasma.
Specimen Stability Information
Specimen Type Temperature Time
​Plasma Frozen​ 14 days​
Rejection Criteria
Gross hemolysis
​Gross lipemia
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Mayo Medical Laboratories​
Monday through Friday​
 2 hours​
Automated Latex Immunoassay (LIA)​
Reference Lab
Mayo Clinic Laboratories
Reference Range Information
Adults: 80-130%
Normal, full-term newborn infants may have decreased levels (> or =35-40%) which reach adult levels by 180 days postnatal.*
Healthy, premature infants (30-36 weeks gestation) may have decreased levels which reach adult levels by 180 days postnatal.*
Interpretation
​Hereditary antithrombin deficiency is much less common than acquired deficiency. Diagnosis of hereditary deficiency requires clinical correlation, testing of both antithrombin activity and antithrombin antigen, and may be aided by repeated testing and by family studies. DNA-based diagnostic testing may be helpful, but is generally not readily available.
 
Acquired antithrombin deficiency may occur in association with a number of conditions (see Clinical Information). The clinical significance (thrombotic risk) of acquired antithrombin deficiency is not well established, but accumulating information suggests possible benefit of antithrombin replacement therapy in carefully selected situations.(4)
 
Increased antithrombin activity has no definite clinical significance.
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​85301 ​ ​ ​ ​
Synonyms/Keywords
​Ref Lab Code: 9031,
Antithrombin III, Immunologic, Plasma, AT III Antigen/Immunologic, AT3 Antigen/Immunologic
Ordering Applications
Ordering Application Description
​COM ​Misc Test
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Plasma​ Citrated Blue Top Tube (BTT)​ ​ 1 mL​ 0.5 mL​ ​
Collection Processing

​Submit sample (platelet poor plasma) in a plastic vial.

1. Spin down, remove plasma, and spin plasma again.
2. Freeze specimen immediately at < or =-40 degrees C, if possible.
Additional Information:
1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.
2. Each coagulation assay requested should have its own vial.
3. Heparin treatment may lower plasma antithrombin.
4. Coagulation testing is highly complex, often requiring the performance of multiple assays and correlation with clinical information. For that reason, we suggest ordering THRMP/83093 Coagulation Consultation, Thrombosis/Hypercoagulability, Blood and Plasma.
Specimen Stability Information
Specimen Type Temperature Time
​Plasma Frozen​ 14 days​
Rejection Criteria
Gross hemolysis
​Gross lipemia
Useful For
​Assessing abnormal results of the antithrombin activity assay. (ATTF/9030 Antithrombin Activity, Plasma), which is recommended as the primary (screening) antithrombin assay
 
Diagnosing antithrombin deficiency, acquired or congenital, in conjunction with measurement of antithrombin activity
 
As an adjunct in the diagnosis and management of carbohydrate-deficient glycoprotein syndromes
Reference Range Information
Adults: 80-130%
Normal, full-term newborn infants may have decreased levels (> or =35-40%) which reach adult levels by 180 days postnatal.*
Healthy, premature infants (30-36 weeks gestation) may have decreased levels which reach adult levels by 180 days postnatal.*
Interpretation
​Hereditary antithrombin deficiency is much less common than acquired deficiency. Diagnosis of hereditary deficiency requires clinical correlation, testing of both antithrombin activity and antithrombin antigen, and may be aided by repeated testing and by family studies. DNA-based diagnostic testing may be helpful, but is generally not readily available.
 
Acquired antithrombin deficiency may occur in association with a number of conditions (see Clinical Information). The clinical significance (thrombotic risk) of acquired antithrombin deficiency is not well established, but accumulating information suggests possible benefit of antithrombin replacement therapy in carefully selected situations.(4)
 
Increased antithrombin activity has no definite clinical significance.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Mayo Medical Laboratories​
Monday through Friday​
 2 hours​
Automated Latex Immunoassay (LIA)​
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​85301 ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.