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22249 Bilirubin, Neonatal

Bilirubin, Neonatal
Test Code: NBILI
Synonyms/Keywords
NBili, Neonatal Bilirubin​
Test Components
Total Bilirubin, Direct Bilirubin
Indirect Bilirubin will be calculated​
Useful For
Elevated serum bilirubin concentrations are associated with the development of yellow staining of the brain, kernicterus, and brain injury resulting in spasticity, hearing loss, and mental retardation.  ​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No Plasma/Serum​ Lithium-heparin Plasma Separator (PST)​, Serum Separator Tube (SST) Lithium or Sodium-heparin Green Top (GTT), Red Top Tube (RTT),
Red or Green Microcollection tube​		 0.1 mL​ 0.1 mL​ 0.1 mL serum or plasma from 0.3mL whole blood​
Collection Processing Instructions
Protect from light and extreme temperatures​
Specimen Stability Information
Specimen Type Temperature Time
Plasma/Serum​ ​ Refrigerate​ 2 days​
Frozen​ >2 days​
Rejection Criteria
​Samples not protected by light
Significantly hemolyzed samples cannot be assayed for Direct Bilirubin​
Interference
Hemolysis will falsely increase Direct Bilirubin.​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Chippewa Falls​ ​​Monday through Friday ​Less than 2 hours​ ​Bichromatic Endpoint Technique/Siemens Dimension
​Diagnostic Treatment Center ​Monday through Sunday​ ​Less than 2 hours​ ​​Bichromatic Endpoint Technique/Siemens Dimension
​Eau Claire ​Monday through Sunday​ ​Less than 2 hours​ ​​Bichromatic Endpoint Technique/Siemens Dimension
​Flambeau Hospital ​Monday through Sunday​ ​Less than 2 hours​ ​​Bichromatic Endpoint Technique/Siemens Dimension
​Lake Hallie ​Monday through Sunday​ ​Less than 2 hours​ ​​Bichromatic Endpoint Technique/Siemens Dimension
​Ladysmith Medical Center​Monday through Sunday​​Less than 2 hours​​Bichromatic Endpoint Technique/Siemens Dimension
​Lakeview Medical Center ​Monday through Sunday​ ​Less than 2 hours​ ​​Bichromatic Endpoint Technique/Siemens Dimension
Marshfield​ Monday through Sunday​ Less than 2 hours​ Diazonium Salt/DPD Endpoint Assay/ Beckman AU
​Merrill ​Monday through Friday ​Less than 2 hours​ ​​Bichromatic Endpoint Technique/Siemens Dimension
​Minocqua ​Monday through Sunday​ ​Less than 2 hours​ ​​Bichromatic Endpoint Technique/Siemens Dimension
Neillsville​​Monday through Sunday​​Less than 2 hours​​Bichromatic Endpoint Technique/Siemens Dimension
​Stevens Point ​Monday through Saturday ​Less than 2 hours ​Bichromatic Endpoint Technique/Siemens Dimension
Wausau​ ​Monday through Sunday​ ​Less than 2 hours​ ​​Bichromatic Endpoint Technique/Siemens Dimension
Reference Range Information
Performing Location Reference Range
All Performing Sites
Total:      0-14 days:  <=11.0 mg/dL
Total: 15 days-<1yr:    <=0.7 mg/dL
Total:           1-<9yr:    <=0.4 mg/dL
Direct:    <=14 days:    <=0.5 mg/dL
Direct:    >=15 days:​    <=0.3 mg/dL
​See Critical Value List for list of current critical values. ​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
82247​ ​ ​ Bilirubin, total  ​ ​
82248​ ​ ​ Bilirubin, direct ​ ​
Synonyms/Keywords
NBili, Neonatal Bilirubin​
Test Components
Total Bilirubin, Direct Bilirubin
Indirect Bilirubin will be calculated​
Ordering Applications
Ordering Application Description
​Centricity ​Bilirubin, Neonatal
​Cerner ​Bilirubin Total Pediatric (COMMON)
​COM ​Bilirubin, Neonate
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No Plasma/Serum​ Lithium-heparin Plasma Separator (PST)​, Serum Separator Tube (SST) Lithium or Sodium-heparin Green Top (GTT), Red Top Tube (RTT),
Red or Green Microcollection tube​		 0.1 mL​ 0.1 mL​ 0.1 mL serum or plasma from 0.3mL whole blood​
Collection Processing
Protect from light and extreme temperatures​
Specimen Stability Information
Specimen Type Temperature Time
Plasma/Serum​ ​ Refrigerate​ 2 days​
Frozen​ >2 days​
Rejection Criteria
​Samples not protected by light
Significantly hemolyzed samples cannot be assayed for Direct Bilirubin​
Interference
Hemolysis will falsely increase Direct Bilirubin.​
Useful For
Elevated serum bilirubin concentrations are associated with the development of yellow staining of the brain, kernicterus, and brain injury resulting in spasticity, hearing loss, and mental retardation.  ​
Test Components
Total Bilirubin, Direct Bilirubin
Indirect Bilirubin will be calculated​
Reference Range Information
Performing Location Reference Range
All Performing Sites
Total:      0-14 days:  <=11.0 mg/dL
Total: 15 days-<1yr:    <=0.7 mg/dL
Total:           1-<9yr:    <=0.4 mg/dL
Direct:    <=14 days:    <=0.5 mg/dL
Direct:    >=15 days:​    <=0.3 mg/dL
​See Critical Value List for list of current critical values. ​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Chippewa Falls​ ​​Monday through Friday ​Less than 2 hours​ ​Bichromatic Endpoint Technique/Siemens Dimension
​Diagnostic Treatment Center ​Monday through Sunday​ ​Less than 2 hours​ ​​Bichromatic Endpoint Technique/Siemens Dimension
​Eau Claire ​Monday through Sunday​ ​Less than 2 hours​ ​​Bichromatic Endpoint Technique/Siemens Dimension
​Flambeau Hospital ​Monday through Sunday​ ​Less than 2 hours​ ​​Bichromatic Endpoint Technique/Siemens Dimension
​Lake Hallie ​Monday through Sunday​ ​Less than 2 hours​ ​​Bichromatic Endpoint Technique/Siemens Dimension
​Ladysmith Medical Center​Monday through Sunday​​Less than 2 hours​​Bichromatic Endpoint Technique/Siemens Dimension
​Lakeview Medical Center ​Monday through Sunday​ ​Less than 2 hours​ ​​Bichromatic Endpoint Technique/Siemens Dimension
Marshfield​ Monday through Sunday​ Less than 2 hours​ Diazonium Salt/DPD Endpoint Assay/ Beckman AU
​Merrill ​Monday through Friday ​Less than 2 hours​ ​​Bichromatic Endpoint Technique/Siemens Dimension
​Minocqua ​Monday through Sunday​ ​Less than 2 hours​ ​​Bichromatic Endpoint Technique/Siemens Dimension
Neillsville​​Monday through Sunday​​Less than 2 hours​​Bichromatic Endpoint Technique/Siemens Dimension
​Stevens Point ​Monday through Saturday ​Less than 2 hours ​Bichromatic Endpoint Technique/Siemens Dimension
Wausau​ ​Monday through Sunday​ ​Less than 2 hours​ ​​Bichromatic Endpoint Technique/Siemens Dimension
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
82247​ ​ ​ Bilirubin, total  ​ ​
82248​ ​ ​ Bilirubin, direct ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.