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22298 Antibody Elution

Antibody Elution
Test Code: DAT
Synonyms/Keywords
​Elution Study, Eluate
Useful For
​Used for investigation of positive DAT, to recover and identify the IgG and/or ABO antibody coating patient cells
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Whole blood​ 6 mL EDTA
Pink Top Tube
(PTT)​
Two 3 mL EDTA Lavender Top Tubes (LTT)
 
Cord blood Sodium-heparin Green Top Tube (GTT) or EDTA Lavender Top (LTT)​
 3 mL ​ 2.5 mL ​ Two 0.5 mL microcollection tubes​
Specimen Stability Information
Specimen Type Temperature Time
Whole Blood​ Refrigerate​ <72 hours old​
Cord Blood​ Refrigerate​ <72 hours old​
Rejection Criteria
Clotted specimen-if DAT  positive, must repeat on EDTA
Sample >72 hours old will yield weaker results- OK​
Serum separator tubes ​
Hemolyzed​
Frozen​
Interference

​False positive DAT results are most often associated with the use of refrigerated, clotted blood samples in which complement components coat RBCs in vitro. Any positive DAT result obtained from a clotted blood sample should be confirmed using a freshly collected EDTA-anticoagulated specimen.

Cells with a positive DAT due to complement alone will normally yield an eluate containing no antibody activity.

Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Sunday​ 2-4 hours​ Gamma ELU-KIT II or LUI FREEZE method​
Test Information
​Contact Transfusion Service with current test results and patient information at 800-222-5835 ext. 16262 before sending specimen. Test is used to identify red cell coating antibodies. This test is to be test requested by Transfusion Service medical director or designee in consultation with local provider. Test is used to identify red cell coating antibodies.
 
Order as DAT and comment ANTIBODY ELUTION
Reference Range Information
Performing Location Reference Range
​Marshfield No reference ranges are available​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​86860 ​ ​ ​ ​
Synonyms/Keywords
​Elution Study, Eluate
Ordering Applications
Ordering Application Description
​Centricity Direct Antiglobulin (DAT, Direct Coombs)​
​Cerner Direct Antiglobulin Test​
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Whole blood​ 6 mL EDTA
Pink Top Tube
(PTT)​
Two 3 mL EDTA Lavender Top Tubes (LTT)
 
Cord blood Sodium-heparin Green Top Tube (GTT) or EDTA Lavender Top (LTT)​
 3 mL ​ 2.5 mL ​ Two 0.5 mL microcollection tubes​
Specimen Stability Information
Specimen Type Temperature Time
Whole Blood​ Refrigerate​ <72 hours old​
Cord Blood​ Refrigerate​ <72 hours old​
Rejection Criteria
Clotted specimen-if DAT  positive, must repeat on EDTA
Sample >72 hours old will yield weaker results- OK​
Serum separator tubes ​
Hemolyzed​
Frozen​
Interference

​False positive DAT results are most often associated with the use of refrigerated, clotted blood samples in which complement components coat RBCs in vitro. Any positive DAT result obtained from a clotted blood sample should be confirmed using a freshly collected EDTA-anticoagulated specimen.

Cells with a positive DAT due to complement alone will normally yield an eluate containing no antibody activity.

Useful For
​Used for investigation of positive DAT, to recover and identify the IgG and/or ABO antibody coating patient cells
Reference Range Information
Performing Location Reference Range
​Marshfield No reference ranges are available​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Sunday​ 2-4 hours​ Gamma ELU-KIT II or LUI FREEZE method​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​86860 ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.