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22320 Antinuclear Antibody Immunofluorescent (ANA-HEp2)

Antinuclear Antibody Immunofluorescent (ANA-HEp2)
Test Code: ANA
Synonyms/Keywords
ANA, FANA, IFA-ANA​
Useful For
Antinuclear antibody (ANA) is a general term used to describe autoantibodies against various cell nuclear proteins. Antibodies specific to DNA show a high disease correlation with systemic lupus erythematosus (SLE). A number of other nuclear and cytoplasmic macromolecules have been detected, and are associated with other connective tissue diseases. Because a number of these antibodies appear to be of diagnostic and/or prognostic use in progressive systemic sclerosis, mixed connective tissue disease, Sjögren's syndrome, polymyositis, and rheumatoid arthritis, ANA testing is no longer considered only a test to rule out SLE. ANA testing is now recognized as a general screening tool for connective tissue disease. ​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum​ Red Top Tube (RTT) or Serum Separator Tube (SST) ​ ​ 0.5 mL​ 0.3 mL​ 0.3 mL​
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ Refrigerate​ <2 days​
Frozen >2 days​
Rejection Criteria
Gross hemolysis
Gross lipemia
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ 1 day​ Indirect Immunofluorescence (IFA)​
Test Information
Test is performed using Hep-2 substrate. Positives are titered at an additional charge and pattern reported. The following patterns will be reported when observed: anti-centromere (discrete speckled), homogeneous, speckled, nucleolar, Proliferating Cell Antigen, and Homogeneous Rim. The presence of a cytoplasmic pattern will also be reported.​
Reference Range Information
Performing Location Reference Range
Marshfield​ Negative: <1:80 ​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86038​ ​ 1​ Screen​ ​
86039​ ​ 1​ Titer, if positive​ ​
Synonyms/Keywords
ANA, FANA, IFA-ANA​
Ordering Applications
Ordering Application Description
​Centricity ​ANA Immunofluorescent (HEp2)
​Cerner ​ANA Immunofluorescent (HEp2)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum​ Red Top Tube (RTT) or Serum Separator Tube (SST) ​ ​ 0.5 mL​ 0.3 mL​ 0.3 mL​
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ Refrigerate​ <2 days​
Frozen >2 days​
Rejection Criteria
Gross hemolysis
Gross lipemia
Useful For
Antinuclear antibody (ANA) is a general term used to describe autoantibodies against various cell nuclear proteins. Antibodies specific to DNA show a high disease correlation with systemic lupus erythematosus (SLE). A number of other nuclear and cytoplasmic macromolecules have been detected, and are associated with other connective tissue diseases. Because a number of these antibodies appear to be of diagnostic and/or prognostic use in progressive systemic sclerosis, mixed connective tissue disease, Sjögren's syndrome, polymyositis, and rheumatoid arthritis, ANA testing is no longer considered only a test to rule out SLE. ANA testing is now recognized as a general screening tool for connective tissue disease. ​
Reference Range Information
Performing Location Reference Range
Marshfield​ Negative: <1:80 ​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ 1 day​ Indirect Immunofluorescence (IFA)​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86038​ ​ 1​ Screen​ ​
86039​ ​ 1​ Titer, if positive​ ​
For most current information refer to the Marshfield Laboratory online reference manual.