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22382 Lyme Test by PCR

Lyme Test by PCR
Test Code: LYMEPCR
Synonyms/Keywords
​Lyme Disease PCR, Borrelia burgdorferi by PCR
Useful For
​The Lyme disease PCR test detects specific DNA sequences of Borrelia burgdorferi in clinical specimens. PCR is most useful when testing synovial fluid of patients with suspected Lyme arthritis in order to establish a primary diagnosis and for monitoring response to therapy. Testing should be limited to patients for which there is a moderate to high risk of Lyme arthritis and have at least an equivocal or positive serologic test for antibodies to Borrelia burgdorferi. PCR on cerebrospinal fluid (CSF) should be requested only on patients with suspected neuroborreliosis who have abnormal CSF findings (elevated white blood cells and protein levels) and serum containing antibodies to B. burgdorferi.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No ​ ​CSF Screw-capped sterile vial​ 1.0 mL​ 0.5 mL​ 0.25 mL​
Synovial Fluid​ Screw-capped sterile vial​ ETDA Lavender Top Tube (LTT)​ 1.0 mL​ 0.5 mL​ 0.25 mL​
Collection Processing Instructions
Refrigerate or freeze.
Avoid freeze / thaw cycles.​
Specimen Stability Information
Specimen Type Temperature Time​
​CSF ​ ​Ambient 24 hrs​
​Refrigerate 7 days
​Synovial Fluids ​Ambient 24 hrs​
​Refrigerate 7 days
Rejection Criteria
Heparinized
​Specimens other than CSF or Synovial Fluid
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield
​CSF: Monday through Sunday
 
Synovial Fluids: Tuesday and Friday
6-8 hours*
 ​Real Time PCR, Taq Man Probe
*Testing performed once/day.
​ ​Sample must be re​ceived in the lab by 0400 to be tested same day.​ ​​ ​ ​ ​
Test Information
This test was developed and its performance characteristics determined by Marshfield Labs. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) to perform high complexity testing.
 
The sensitivity of PCR for the diagnosis of Borrelia burgdorferi infection is variable depending on the stage of the disease and presence or absence of prior antimicrobial therapy. A negative result does not exclude active infection. PCR results should be interpreted in conjunction with all of the clinical, laboratory, and epidemiologic information that is available.​
Reference Range Information
Performing Location Reference Range
​Marshfield ​Negative for Borrelia burgdorferi DNA by Rapid PCR.
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87476 ​ ​ ​ ​
Classification
This test was developed and its performance characteristics determined by Marshfield Labs. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) to perform high complexity testing.​
Synonyms/Keywords
​Lyme Disease PCR, Borrelia burgdorferi by PCR
Ordering Applications
Ordering Application Description
​Centricity ​Lyme-PCR
​Cerner ​Lyme Disease by PCR
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No ​ ​CSF Screw-capped sterile vial​ 1.0 mL​ 0.5 mL​ 0.25 mL​
Synovial Fluid​ Screw-capped sterile vial​ ETDA Lavender Top Tube (LTT)​ 1.0 mL​ 0.5 mL​ 0.25 mL​
Collection Processing
Refrigerate or freeze.
Avoid freeze / thaw cycles.​
Specimen Stability Information
Specimen Type Temperature Time​
​CSF ​ ​Ambient 24 hrs​
​Refrigerate 7 days
​Synovial Fluids ​Ambient 24 hrs​
​Refrigerate 7 days
Rejection Criteria
Heparinized
​Specimens other than CSF or Synovial Fluid
Useful For
​The Lyme disease PCR test detects specific DNA sequences of Borrelia burgdorferi in clinical specimens. PCR is most useful when testing synovial fluid of patients with suspected Lyme arthritis in order to establish a primary diagnosis and for monitoring response to therapy. Testing should be limited to patients for which there is a moderate to high risk of Lyme arthritis and have at least an equivocal or positive serologic test for antibodies to Borrelia burgdorferi. PCR on cerebrospinal fluid (CSF) should be requested only on patients with suspected neuroborreliosis who have abnormal CSF findings (elevated white blood cells and protein levels) and serum containing antibodies to B. burgdorferi.
Reference Range Information
Performing Location Reference Range
​Marshfield ​Negative for Borrelia burgdorferi DNA by Rapid PCR.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield
​CSF: Monday through Sunday
 
Synovial Fluids: Tuesday and Friday
6-8 hours*
 ​Real Time PCR, Taq Man Probe
*Testing performed once/day.
​ ​Sample must be re​ceived in the lab by 0400 to be tested same day.​ ​​ ​ ​ ​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87476 ​ ​ ​ ​
Classification
This test was developed and its performance characteristics determined by Marshfield Labs. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) to perform high complexity testing.​
For most current information refer to the Marshfield Laboratory online reference manual.