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22633 Flow Cell Phenotype Other

Flow Cell Phenotype Other
Test Code: FLOW
Synonyms/Keywords
Flow Cytometry, Immunophenotyping, Lymphoma / Leukemia Panel, CD4, CD4/CD8​
Useful For
Diagnosis and monitoring of patients with leukemia and lymphoma.​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Body fluid​ Sodium-heparin Green Top Tube (GTT)​ ​ ​4 mL ​Dependent on WBC Dependent on WBC​
No​ ​CSF Sodium-heparin Green Top Tube (GTT)​; add equal volume of RPMI or HBSS if available ​ 2 mL​ Dependent on WBC​ ​Dependent on WBC
​No ​Tissue ​Container/Tube filled with RPMI or HBSS ​ ​ ​ ​
Collection Processing Instructions
Acceptable specimens include: Fresh Tissue (in sterile RPMI-1640, Hank's Balanced Salt Solution, Cytogenetics Transport Media, or sterile saline); or Body Fluids (submit in sodium heparin). If tissue is large, cut into several smaller pieces.  Specimen must arrive in Flow Cytometry within 36 hours of collection. Notify Flow Cytometry (800-222-5835, ext. 16385) of specimen arrival in lab. Cells in specimen must be viable.​
Specimen Stability Information
Specimen Type Temperature Time
Tissue​ Room temperature​ 48-72 hours​
Body fluid​ Room temperature​ 48-72 hours​
​CSF ​Room temperature ​48-72 hours​
Rejection Criteria
Fixed specimens
Specimens more than 72 hours old​
Frozen or refrigerated​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday, days only​ 3 days​ Flow Cytometry​
Test Information
Markers are selected based upon available clinical history and morphology per pathology protocol.
 
This test was developed and its performance characteristics determined by Marshfield Labs.  It has not been cleared or approved by the U.S. Food and Drug Administration.  The FDA has determined that such clearance or approval is not necessary.  This test is for clinical purposes.  It should not be regarded as investigational or for research.  This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity clinical testing.
Reference Range Information
Performing Location Reference Range
Marshfield​ Interpretive report​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
88184​ ​ ​ Flow cytometry, first marker ​ ​
88185​ ​ ​ Additional marker, each ​ ​
88187​ ​ ​ Interpretation, 2 to 8 markers ​ ​
88188​ ​ ​ Interpretation, 9 to 15 markers ​ ​
88189​ ​ ​ Interpretation, 16 or more markers ​ ​
Classification
This test was developed and its performance characteristics determined by Marshfield Labs.  It has not been cleared or approved by the U.S. Food and Drug Administration.  The FDA has determined that such clearance or approval is not necessary.  This test is for clinical purposes.  It should not be regarded as investigational or for research.  This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity clinical testing.
​
Synonyms/Keywords
Flow Cytometry, Immunophenotyping, Lymphoma / Leukemia Panel, CD4, CD4/CD8​
Ordering Applications
Ordering Application Description
​Centricity ​Flow Cell Phenotyping Other
​Cerner ​Flow Cell Phenotype Other
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Body fluid​ Sodium-heparin Green Top Tube (GTT)​ ​ ​4 mL ​Dependent on WBC Dependent on WBC​
No​ ​CSF Sodium-heparin Green Top Tube (GTT)​; add equal volume of RPMI or HBSS if available ​ 2 mL​ Dependent on WBC​ ​Dependent on WBC
​No ​Tissue ​Container/Tube filled with RPMI or HBSS ​ ​ ​ ​
Collection Processing
Acceptable specimens include: Fresh Tissue (in sterile RPMI-1640, Hank's Balanced Salt Solution, Cytogenetics Transport Media, or sterile saline); or Body Fluids (submit in sodium heparin). If tissue is large, cut into several smaller pieces.  Specimen must arrive in Flow Cytometry within 36 hours of collection. Notify Flow Cytometry (800-222-5835, ext. 16385) of specimen arrival in lab. Cells in specimen must be viable.​
Specimen Stability Information
Specimen Type Temperature Time
Tissue​ Room temperature​ 48-72 hours​
Body fluid​ Room temperature​ 48-72 hours​
​CSF ​Room temperature ​48-72 hours​
Rejection Criteria
Fixed specimens
Specimens more than 72 hours old​
Frozen or refrigerated​
Useful For
Diagnosis and monitoring of patients with leukemia and lymphoma.​
Reference Range Information
Performing Location Reference Range
Marshfield​ Interpretive report​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday, days only​ 3 days​ Flow Cytometry​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
88184​ ​ ​ Flow cytometry, first marker ​ ​
88185​ ​ ​ Additional marker, each ​ ​
88187​ ​ ​ Interpretation, 2 to 8 markers ​ ​
88188​ ​ ​ Interpretation, 9 to 15 markers ​ ​
88189​ ​ ​ Interpretation, 16 or more markers ​ ​
Classification
This test was developed and its performance characteristics determined by Marshfield Labs.  It has not been cleared or approved by the U.S. Food and Drug Administration.  The FDA has determined that such clearance or approval is not necessary.  This test is for clinical purposes.  It should not be regarded as investigational or for research.  This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity clinical testing.
​
For most current information refer to the Marshfield Laboratory online reference manual.