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22752 HLA-B27

HLA-B27
Test Code: B27
Synonyms/Keywords
Flow Cytometry, B27, B27-SO​
Useful For
HLA-B27 antigen is strongly associated with ankylosing spondylitis, a chronic inflammatory disease of the axial musculoskeletal system and a few other rheumatic disorders such as Reiter’s syndrome, acute anterior uveitis, and inflammatory bowel disease.  HLA-B27 testing is routinely used to screen for ankylosing spondylitis since 90% of patients with ankylosing spondylitis have the HLA-B27 surface antigen compared to only 8% of healthy individuals.​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Whole blood​
EDTA Lavender Top Tube (LTT) or
EDTA Pink Top Tube (PTT)​
Sodium-heparin Green Top Tube (GTT)
ACD Soln. A Yellow Top Tube​
 1 mL​ 1 mL​ 0.5 mL​
Collection Processing Instructions
If the results are indeterminate, a confirmatory test is needed. Current sample may be used for confirmatory testing if requested within 10 days of draw date.  Order as a MISC and add comment B27-SO. Call Flow Cytometry (800-222-5835 ext 16385) for more information.​
Specimen Stability Information
Specimen Type Temperature Time
Whole blood​ Room temperature​ 5 days ​
Rejection Criteria
Refrigerated or frozen
Fixed specimens​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday, days only​ 4 days​ Flow Cytometry​
Reference Range Information
Performing Location Reference Range
Marshfield​ Negative​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86812​ ​ ​ ​ ​
Synonyms/Keywords
Flow Cytometry, B27, B27-SO​
Ordering Applications
Ordering Application Description
​Centricity ​HLA B27
​Cerner ​HLA B27
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Whole blood​
EDTA Lavender Top Tube (LTT) or
EDTA Pink Top Tube (PTT)​
Sodium-heparin Green Top Tube (GTT)
ACD Soln. A Yellow Top Tube​
 1 mL​ 1 mL​ 0.5 mL​
Collection Processing
If the results are indeterminate, a confirmatory test is needed. Current sample may be used for confirmatory testing if requested within 10 days of draw date.  Order as a MISC and add comment B27-SO. Call Flow Cytometry (800-222-5835 ext 16385) for more information.​
Specimen Stability Information
Specimen Type Temperature Time
Whole blood​ Room temperature​ 5 days ​
Rejection Criteria
Refrigerated or frozen
Fixed specimens​
Useful For
HLA-B27 antigen is strongly associated with ankylosing spondylitis, a chronic inflammatory disease of the axial musculoskeletal system and a few other rheumatic disorders such as Reiter’s syndrome, acute anterior uveitis, and inflammatory bowel disease.  HLA-B27 testing is routinely used to screen for ankylosing spondylitis since 90% of patients with ankylosing spondylitis have the HLA-B27 surface antigen compared to only 8% of healthy individuals.​
Reference Range Information
Performing Location Reference Range
Marshfield​ Negative​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday, days only​ 4 days​ Flow Cytometry​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86812​ ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.