Mumps Virus Antibody, IgG and IgM, Separate Determinations (MMPGM)

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Marshfield Clinic Public WebSite

Test Code: MUMPDSO

Overview
Ordering
Specimen
Performing
Clinical/Interpretive
Contacts
Coding

Synonyms/Keywords

Mumps Serology, Mumps Antibody

Useful For

Laboratory diagnosis of mumps virus infection.

Determination of postimmunization immune response of individuals to the mumps vaccine.

Documentation of previous infection with mumps virus in an individual with no previous record of immunization to mumps virus.

Specimen Requirements

Specimen Type	Preferred Container/Tube	Acceptable Container/Tube	Specimen Volume	Specimen Minimum Volume (allows for 1 repeat)	Pediatric Minimum Volume (no repeat)
Serum	Red Top Tube (RTT)	Serum Separator Tube (SST)	1.0 mL	0.9 mL

Specimen Stability Information

Specimen Type	Temperature	Time
Serum	Refrigerated (preferred)	14 days
	Frozen	14 days

Rejection Criteria

Hemolysis	Mild OK; Gross reject
Lipemia	Mild OK; Gross reject
Other	Heat-inactivated specimen

Performing Laboratory Information

Performing Location	Day(s) Test Performed	Analytical Time	Methodology/Instrumentation
Mayo Medical Laboratories	Monday through Saturday, 9 a.m.	Same day/1 day	MMPM: Enzyme Immunoassay (EIA) MPPG: Multiplex Flow Immunoassay

Reference Lab

Mayo Clinic Laboratories

Test Information

Serum specimens obtained during the acute phase of infection or soon after vaccination may be negative for IgM- or IgG-class antibodies by serological tests.

All positive IgM results must be interpreted cautiously as some false-positive results or heterotypical responses of the IgM have been seen in the serum of pregnant women or in patients with an acute infection caused by cytomegalovirus, herpes simplex virus, measles, rubella, or parvovirus.

Testing for IgM-class antibodies to mumps virus should be limited to patients with a clinically compatible disease.

Mumps virus shares antigenic relationships with other viruses of the paramyxovirus group; therefore serologic cross-reactions are possible, but uncommon with this test procedure.

IgG-class antibodies to mumps virus may be present in serum specimens from individuals who have received blood products within the past several months, but have not been immunized or experienced past infection with this virus.

Reference Range Information

IgM

Negative (reported as positive, equivocal, or negative)

Index value 0.00-0.79=negative

IgG

Vaccinated: positive

Unvaccinated: negative

Interpretation

A positive IgG result coupled with a positive IgM result suggests recent infection with mumps virus. This result should not be used alone to diagnose mumps infection and should be interpreted in the context of clinical presentation.

A positive IgG result coupled with a negative IgM result indicates previous vaccination to or infection with mumps virus. These individuals are considered to have protective immunity to reinfection.

A negative IgG result coupled with a negative IgM result indicates the absence of prior exposure to mumps virus and nonimmunity. However, a negative result does not rule-out mumps infection or response to vaccination. The specimen may have been drawn before the appearance of detectable antibodies. Negative results in suspected early mumps infection or within a week following vaccination should be followed by testing a new serum specimen in 2 to 3 weeks.

Equivocal results should be followed up with testing of a new serum specimen within 10 to 14 days.

Outreach CPTs

CPT	Modifier (if needed)	Quantity	Description	Comments
86735			Mumps, IgG
86735			Mumps, IgM