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22962 Porphyrin Evaluation, Whole Blood (PEE)

Porphyrin Evaluation, Whole Blood (PEE)
Test Code: MISC
Synonyms/Keywords
​Ref Lab Code: 88886, Coproporphyrin, Erythrocyte Porphyrin, Erythropoietic Protoporphyria (EPP), Free Erythrocyte Porphyrin (FEP), Heptacarboxyl Porphyrin, Pentacarboxyl Porphyrin, Protoporphyrin, Protoporphyrins, Total, Erythrocytes, RBC Porphyrins, Red Blood Cell Porphyrins, Uroporphyrin, Hexacarboxyl Porphyrin, Congenital Erythropoietic Porphyria (CEP)
Useful For
Diagnosis of erythropoietic protoporphyria and congenital erythropoietic porphyria
 
Evaluation of chronic intoxication from exposure to a variety of agents including heavy metals and chemicals
 
Differentiating iron-deficiency anemia from other causes of elevated porphyrin levels
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​All porphyrin tests on whole blood can be performed on 1 draw tube.
Specimen must arrive within 72 hours of draw. ​
Whole blood​ ​Heparin Green Top Tube (GTT) ​Metal free, Dark Blue Top Tube (BTT) or Lithium Heparin Green Top (GTT) ​Full Tube ​3 mL
Collection Processing Instructions
1. Patient should abstain from alcohol for 24 hours.
2. Immediately place specimen on wet ice.
Additional Information: Include a list of medications the patient is currently taking.
Specimen Stability Information
Specimen Type Temperature Time
Whole blood ​Refrigerated ​7 days
Rejection Criteria
Hemolysis Mild reject; Gross reject
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Medical Laboratories Monday through Friday​ ​3 days (not reported on Saturday or Sunday
HPLC​
Reference Lab
Test Information
​Testing begins with total RBC porphyrins. If the result is <80mcg/dL, it is normal and testing is complete. If the total RBC porphyrin value is >80 mcg/dL, additional testing (fractionation assays) will be perfomed at an additional charge to identify the porphyrin involved.
Reference Range Information
Interpretive report
Interpretation
An elevation of total RBC porphyrins can be an expression of a primary or secondary defect in the biosynthesis of heme. However, the specific type of porphyria cannot be determined by total porphyrin analysis alone.
 
Total RBC porphyrin values >80 mcg/dL suggest the existence of an intoxication problem or a metabolic problem that involves accelerated erythropoiesis. If the total RBC porphyrin concentration is elevated, additional testing (fractionation assays) is required to identify and quantify the specific affected porphyrin. Increased total RBC porphyrin concentrations may be due to:
-Free protoporphyrin, the predominant form that is elevated in patients with erythropoietic protoporphyria
-Zinc protoporphyrin, elevated in patients with heavy metal intoxication or iron deficiency anemia
-Uroporphyrin and coproporphyrin, preferentially elevated in patients with congenital erythropoietic porphyria
 
A written interpretation is included with all reports. When abnormal results are detected, a detailed interpretation is given, including:
-An overview of the results and their significance
-Elements of a differential diagnosis
-Recommendations for additional biochemical testing
 
A normal RBC porphyrin evaluation does not rule out other forms of porphyria including porphyria cutanea tarda (PCT), acute intermittent porphyria (AIP), hereditary coproporphyria (HCP), and variegate porphyria (VP).
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
84311​ ​1 Spectrophotometry
​82542 ​1 Chromatography (if appropriate)
Synonyms/Keywords
​Ref Lab Code: 88886, Coproporphyrin, Erythrocyte Porphyrin, Erythropoietic Protoporphyria (EPP), Free Erythrocyte Porphyrin (FEP), Heptacarboxyl Porphyrin, Pentacarboxyl Porphyrin, Protoporphyrin, Protoporphyrins, Total, Erythrocytes, RBC Porphyrins, Red Blood Cell Porphyrins, Uroporphyrin, Hexacarboxyl Porphyrin, Congenital Erythropoietic Porphyria (CEP)
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​All porphyrin tests on whole blood can be performed on 1 draw tube.
Specimen must arrive within 72 hours of draw. ​
Whole blood​ ​Heparin Green Top Tube (GTT) ​Metal free, Dark Blue Top Tube (BTT) or Lithium Heparin Green Top (GTT) ​Full Tube ​3 mL
Collection Processing
1. Patient should abstain from alcohol for 24 hours.
2. Immediately place specimen on wet ice.
Additional Information: Include a list of medications the patient is currently taking.
Specimen Stability Information
Specimen Type Temperature Time
Whole blood ​Refrigerated ​7 days
Rejection Criteria
Hemolysis Mild reject; Gross reject
Useful For
Diagnosis of erythropoietic protoporphyria and congenital erythropoietic porphyria
 
Evaluation of chronic intoxication from exposure to a variety of agents including heavy metals and chemicals
 
Differentiating iron-deficiency anemia from other causes of elevated porphyrin levels
Reference Range Information
Interpretive report
Interpretation
An elevation of total RBC porphyrins can be an expression of a primary or secondary defect in the biosynthesis of heme. However, the specific type of porphyria cannot be determined by total porphyrin analysis alone.
 
Total RBC porphyrin values >80 mcg/dL suggest the existence of an intoxication problem or a metabolic problem that involves accelerated erythropoiesis. If the total RBC porphyrin concentration is elevated, additional testing (fractionation assays) is required to identify and quantify the specific affected porphyrin. Increased total RBC porphyrin concentrations may be due to:
-Free protoporphyrin, the predominant form that is elevated in patients with erythropoietic protoporphyria
-Zinc protoporphyrin, elevated in patients with heavy metal intoxication or iron deficiency anemia
-Uroporphyrin and coproporphyrin, preferentially elevated in patients with congenital erythropoietic porphyria
 
A written interpretation is included with all reports. When abnormal results are detected, a detailed interpretation is given, including:
-An overview of the results and their significance
-Elements of a differential diagnosis
-Recommendations for additional biochemical testing
 
A normal RBC porphyrin evaluation does not rule out other forms of porphyria including porphyria cutanea tarda (PCT), acute intermittent porphyria (AIP), hereditary coproporphyria (HCP), and variegate porphyria (VP).
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Medical Laboratories Monday through Friday​ ​3 days (not reported on Saturday or Sunday
HPLC​
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
84311​ ​1 Spectrophotometry
​82542 ​1 Chromatography (if appropriate)
For most current information refer to the Marshfield Laboratory online reference manual.