This test requires three samples:
1. 6.0 mL whole blood – Pink Top Tube (PTT) used for ABO, Rh factor and Antibody Screen.
2. 3.0 mL whole blood – EDTA Lavender Top Tube (LTT) used for CBC with automated differential. 2 peripheral
blood smears are also required.
3. 3.0 mL serum – Red Top Tube (RTT) used for Syphilis Antibody IgG, Rubella Antibody and Hepatitis B Surface
Antigen.
Processing Instructions:
-Samples collected in a (RTT) must be removed from the cells within one hour for storage or transport.
- Blood smears must be prepared within two hours of collection. Label with computer generated label or use
pencil to label with patients full name and date. Store slides at room temperature.
High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with this assay (HAA). It is recommended that patients refrain from consuming any multivitamin or supplement containing biotin for at least 72 hours prior to collection of a blood sample.