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23001 Prothrombin Gene Mutation

Prothrombin Gene Mutation
Test Code: PTG
Synonyms/Keywords
​Factor II Mutation, PTG 20210
Useful For
​People with prothrombin G20210A or factor V Leiden (G1691A) mutations have a higher than average risk of developing a type of blood clot called a deep venous thrombosis (DVT).  Prothrombin G20210A and Factor V Leiden mutation tests are ordered when it is suspected that a person has an inherited risk factor for thrombosis, for example, in someone who has a first DVT or venous thromboembolism (VTE) before age 50 or in an unusual part of the body.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No ​Whole Blood EDTA Lavender Top Tube (LTT)​ ​Citrated Blue Top Tube (BTT) 1.0 mL​ 1.0 mL​ 0.5 mL​
Collection Processing Instructions
​This tube should be drawn at least 90% full. Invert tube completely three or four times to mix. Do Not Shake. The blood specimen tube must not be opened. Uncentrifuged specimens are preferred.
Specimen Stability Information
Specimen Type Temperature Time
​Whole Blood ​Refrigerate ​<= 7 days
Rejection Criteria
Clotted
​> 7 days old
​Specimen tubes that have been opened
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Thursday 6 hours​* ​PCR/Hybridization Probe
​*Testing performed once per week.  Sample must be received in the lab by noon Wednesday to be tested on Thursday. ​ ​ ​
Test Information

​Heterozygous for Prothrombin G20210A: the relative risk of venous thrombosis is about 3-fold that of the general population. Other conditions, either acquired or inherited, may act synergistically to increase an individual's thrombotic risk.

Homozygous for Prothrombin G20210A:  This is a rare finding occurring roughly 1 in 10,000 people of European descent.  It probably confers some degrees of hypercoagulability, but there are too few cases to date to calculate risk.

Reference Range Information
Performing Location Reference Range
​Marshfield ​Individuals are interpreted as: Heterozygous, Homozygous, or Normal (no evidence of PT G20210A).
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​81240 ​ ​ ​ ​
Synonyms/Keywords
​Factor II Mutation, PTG 20210
Ordering Applications
Ordering Application Description
​Centricity ​Prothrombin G20210A Gene
​Cerner ​Prothrombin Gene Mutation 20210A
​COM ​Prothrombin G20210A Gene
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No ​Whole Blood EDTA Lavender Top Tube (LTT)​ ​Citrated Blue Top Tube (BTT) 1.0 mL​ 1.0 mL​ 0.5 mL​
Collection Processing
​This tube should be drawn at least 90% full. Invert tube completely three or four times to mix. Do Not Shake. The blood specimen tube must not be opened. Uncentrifuged specimens are preferred.
Specimen Stability Information
Specimen Type Temperature Time
​Whole Blood ​Refrigerate ​<= 7 days
Rejection Criteria
Clotted
​> 7 days old
​Specimen tubes that have been opened
Useful For
​People with prothrombin G20210A or factor V Leiden (G1691A) mutations have a higher than average risk of developing a type of blood clot called a deep venous thrombosis (DVT).  Prothrombin G20210A and Factor V Leiden mutation tests are ordered when it is suspected that a person has an inherited risk factor for thrombosis, for example, in someone who has a first DVT or venous thromboembolism (VTE) before age 50 or in an unusual part of the body.
Reference Range Information
Performing Location Reference Range
​Marshfield ​Individuals are interpreted as: Heterozygous, Homozygous, or Normal (no evidence of PT G20210A).
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Thursday 6 hours​* ​PCR/Hybridization Probe
​*Testing performed once per week.  Sample must be received in the lab by noon Wednesday to be tested on Thursday. ​ ​ ​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​81240 ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.