Skip Ribbon Commands
Skip to main content
Sign In
Navigate Up

23109 B-Type Natriuretic Peptide

B-Type Natriuretic Peptide
Test Code: BNP
Synonyms/Keywords
​BNP, Brain-type Natriuretic peptide
Useful For
​This test is used as an aid in the diagnosis of congestive heart failure.
Specimen Requirements
 
 
 
 
 
 
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​

Plasma​

 

(Performing Site-Marshfield Center Lab)

EDTA Pink Top Tube (PTT)​EDTA Lavender Top Tube (LTT)​1.0 mL​ Plasma
0.5 mL​
Plasma
0.3 mL​
Plasma
​No

​Whole Blood

 

(Performing Site-Marshfield Regional Center Labs)

​EDTA Pink Top Tube (PTT)​​EDTA Lavender Top Tube (LTT)​
​1.0 mL
Whole Blood
​0.5 mL
Whole Blood
​0.3 mL
Whole Blood
Collection Processing Instructions

Marshfield Center Lab: Plasma must be separated from red cells within 7 hours of collection. If assay cannot not be performed within 7 hours, plasma may be stored up to 24 hours refrigerated, or at -20 degrees Celsius for longer storage. Preferred storage is frozen. For add-on (U-have) testing, EDTA whole blood samples (pink or lavender top tube) stored up to 7 hours at 2-25 degrees C may be used to obtain the plasma sample.

Regional Center Labs: Whole blood must be analyzed within 7 hours of collection. If assay cannot not be performed within 7 hours of collection, then centrifuge and remove plasma within 7 hours of collection, and send to Marshfield Center lab for testing. Plasma may be stored up to 24 hours refrigerated, or at -20 degrees Celsius for longer storage. Preferred storage is frozen. For add-on (U-have) testing, EDTA whole blood samples (pink or lavender top tube) stored up to 7 hours at 2-25 degrees C may be used to obtain the plasma sample.

Specimen Stability Information
Specimen Type Temperature Time
EDTA Whole Blood​ Ambient​ 7 hours
Refrigerate 7 hours
EDTA Plasma​ ​ ​ Ambient 7 hours
​Refrigerate ​24 hours
​Frozen at -20 deg Celsius ​> 24 hours (up to 3 months)
Rejection Criteria
​
Whole Blood > 7 hours
Room Temperature Plasma > 7 hours
Refrigerated Plasma > 24 hours
Interference
​Hemolyzed specimens should be avoided whenever possible. However, hemoglobin (up to 500 mg/dL), triglycerides (triolein up to 3000 mg/dL), bilirubin (up to 20 mg/dL) or human serum albumin (up to 1500 mg/L) do not interfere with the recovery of BNP per Biosite literature.
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Chippewa Falls​ ​Monday through Friday ​Less than 2 hours​ ​Fluorescence Immunoassay/Triage Meter Alere
​Diagnostic Treatment Center ​Monday through Sunday​ ​Less than 2 hours​ ​​Fluorescence Immunoassay/Triage Meter Alere
​Eau Claire ​Monday through Sunday​ ​Less than 2 hours​ ​​Fluorescence Immunoassay/Triage Meter Alere
​Flambeau Hospital ​Monday through Sunday​ ​Less than 2 hours​ ​​Fluorescence Immunoassay/Triage Meter Alere
​Ladysmith Medical Center ​​Monday through Sunday ​Less than 2 hours​ ​​Fluorescence Immunoassay/Triage Meter Alere
​Lake Hallie ​Monday through Sunday​ ​Less than 2 hours​ ​​Fluorescence Immunoassay/Triage Meter Alere
​Lakeview Medical Center ​Monday through Sunday​ ​Less than 2 hours​ ​​Fluorescence Immunoassay/Triage Meter Alere
Marshfield​ Monday through Sunday​ Less than 2 hours​ Chemiluminescent/Siemens Centaur
Minocqua​ ​Monday through Sunday​ ​Less than 2 hours​ ​​Fluorescence Immunoassay/Triage Meter Alere
​Neillsville​Monday through Sunday​​Less than 2 hours​​Fluorescence Immunoassay/Triage Meter Alere
Stevens Point​​Monday through Sunday​​Less than 2 hours​​​​Fluorescence Immunoassay/Triage Meter Alere
​Wausau ​Monday through Sunday​ ​Less than 2 hours​ ​​Fluorescence Immunoassay/Triage Meter Alere
​Wisconsin Rapids ​Monday through Sunday ​Less than 2 hours ​Fluorescence Immunoassay/Triage Meter Alere
Reference Range Information
Performing Location Reference Range
All Performing Sites using Triage Meter Alere <=100 pg/mL​
​Marshfield ​<=80 pg/mL
Interpretation
​Normal values <100 pg/mL. The decision threshold of 100 pg/mL translates into a general specificity greater than 95%, i.e. less than 5% expected false positives in individuals without Congestive Heart Failure (CHF).
BNP levels in CHF:
Less than 200: Likely compensated
200-400: Moderate CHF
Greater than 400: Severe CHF.
 
Normal values <80 pg/mL:  The decision threshold for predicting survival and future heart failure in patients with acute coronary syndromes is 80 pg/mL.
The decision threshold for determining heart failure in apparently healthy individuals is 100 pg/mL.  This BNP value translates into a general specificity of the test of greater than 97%.
Less than 200:  Likely compensated
200-400:  Moderate CHF
Greater than 400:  Severe CHF
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
83880​ ​ ​ ​ ​
Synonyms/Keywords
​BNP, Brain-type Natriuretic peptide
Ordering Applications
Ordering Application Description
​Centricity

​a. B-Type Natriuretic Peptide

b. BNP

​Cerner ​B-Type Natriuretic Peptide (COMMON)
​COM ​B-type Natriuretic Peptide-BNP
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
 
 
 
 
 
 
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​

Plasma​

 

(Performing Site-Marshfield Center Lab)

EDTA Pink Top Tube (PTT)​EDTA Lavender Top Tube (LTT)​1.0 mL​ Plasma
0.5 mL​
Plasma
0.3 mL​
Plasma
​No

​Whole Blood

 

(Performing Site-Marshfield Regional Center Labs)

​EDTA Pink Top Tube (PTT)​​EDTA Lavender Top Tube (LTT)​
​1.0 mL
Whole Blood
​0.5 mL
Whole Blood
​0.3 mL
Whole Blood
Collection Processing

Marshfield Center Lab: Plasma must be separated from red cells within 7 hours of collection. If assay cannot not be performed within 7 hours, plasma may be stored up to 24 hours refrigerated, or at -20 degrees Celsius for longer storage. Preferred storage is frozen. For add-on (U-have) testing, EDTA whole blood samples (pink or lavender top tube) stored up to 7 hours at 2-25 degrees C may be used to obtain the plasma sample.

Regional Center Labs: Whole blood must be analyzed within 7 hours of collection. If assay cannot not be performed within 7 hours of collection, then centrifuge and remove plasma within 7 hours of collection, and send to Marshfield Center lab for testing. Plasma may be stored up to 24 hours refrigerated, or at -20 degrees Celsius for longer storage. Preferred storage is frozen. For add-on (U-have) testing, EDTA whole blood samples (pink or lavender top tube) stored up to 7 hours at 2-25 degrees C may be used to obtain the plasma sample.

Specimen Stability Information
Specimen Type Temperature Time
EDTA Whole Blood​ Ambient​ 7 hours
Refrigerate 7 hours
EDTA Plasma​ ​ ​ Ambient 7 hours
​Refrigerate ​24 hours
​Frozen at -20 deg Celsius ​> 24 hours (up to 3 months)
Rejection Criteria
​
Whole Blood > 7 hours
Room Temperature Plasma > 7 hours
Refrigerated Plasma > 24 hours
Interference
​Hemolyzed specimens should be avoided whenever possible. However, hemoglobin (up to 500 mg/dL), triglycerides (triolein up to 3000 mg/dL), bilirubin (up to 20 mg/dL) or human serum albumin (up to 1500 mg/L) do not interfere with the recovery of BNP per Biosite literature.
Useful For
​This test is used as an aid in the diagnosis of congestive heart failure.
Reference Range Information
Performing Location Reference Range
All Performing Sites using Triage Meter Alere <=100 pg/mL​
​Marshfield ​<=80 pg/mL
Interpretation
​Normal values <100 pg/mL. The decision threshold of 100 pg/mL translates into a general specificity greater than 95%, i.e. less than 5% expected false positives in individuals without Congestive Heart Failure (CHF).
BNP levels in CHF:
Less than 200: Likely compensated
200-400: Moderate CHF
Greater than 400: Severe CHF.
 
Normal values <80 pg/mL:  The decision threshold for predicting survival and future heart failure in patients with acute coronary syndromes is 80 pg/mL.
The decision threshold for determining heart failure in apparently healthy individuals is 100 pg/mL.  This BNP value translates into a general specificity of the test of greater than 97%.
Less than 200:  Likely compensated
200-400:  Moderate CHF
Greater than 400:  Severe CHF
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Chippewa Falls​ ​Monday through Friday ​Less than 2 hours​ ​Fluorescence Immunoassay/Triage Meter Alere
​Diagnostic Treatment Center ​Monday through Sunday​ ​Less than 2 hours​ ​​Fluorescence Immunoassay/Triage Meter Alere
​Eau Claire ​Monday through Sunday​ ​Less than 2 hours​ ​​Fluorescence Immunoassay/Triage Meter Alere
​Flambeau Hospital ​Monday through Sunday​ ​Less than 2 hours​ ​​Fluorescence Immunoassay/Triage Meter Alere
​Ladysmith Medical Center ​​Monday through Sunday ​Less than 2 hours​ ​​Fluorescence Immunoassay/Triage Meter Alere
​Lake Hallie ​Monday through Sunday​ ​Less than 2 hours​ ​​Fluorescence Immunoassay/Triage Meter Alere
​Lakeview Medical Center ​Monday through Sunday​ ​Less than 2 hours​ ​​Fluorescence Immunoassay/Triage Meter Alere
Marshfield​ Monday through Sunday​ Less than 2 hours​ Chemiluminescent/Siemens Centaur
Minocqua​ ​Monday through Sunday​ ​Less than 2 hours​ ​​Fluorescence Immunoassay/Triage Meter Alere
​Neillsville​Monday through Sunday​​Less than 2 hours​​Fluorescence Immunoassay/Triage Meter Alere
Stevens Point​​Monday through Sunday​​Less than 2 hours​​​​Fluorescence Immunoassay/Triage Meter Alere
​Wausau ​Monday through Sunday​ ​Less than 2 hours​ ​​Fluorescence Immunoassay/Triage Meter Alere
​Wisconsin Rapids ​Monday through Sunday ​Less than 2 hours ​Fluorescence Immunoassay/Triage Meter Alere
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
83880​ ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.