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23125 HIV-1 Direct Antigen (ICD) Assay

HIV-1 Direct Antigen (ICD) Assay
Test Code: HIV-AG
Synonyms/Keywords
​Human Immunodeficiency Virus Type I (HIV-1) p24 Antigen Assay, p24 Antigen
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Serum​ Red Top Tube (RTT)​ Serum Separator Tube (SST)​ 1.0 mL​ ​0.5 mL ​
Collection Processing Instructions
​Centrifuge and remove serum from cells within 24 hours of collection. Send serum frozen in a screw-capped plastic vial.
Specimen Stability Information
Specimen Type Temperature
​Serum Frozen​
Rejection Criteria
Heat-inactivated specimens
Hemolyzed samples​
Specimens containing particulate matter or precipitate ​
Specimens other than serum ​
​Cadaver specimens
Diluted specimens ​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Focus Diagnostics​ ​ 2 to 6 days​ ELISA (Enzyme Linked Immunosorbent Assay) ​
Reference Lab
Focus Diagnostics
Test Information
The measurement of HIV p24 (core) antigen is performed by the Immune Complex Dissociation (ICD) modification of the standard p24 antigen assay. This modification results in a significant increase in the sensitivity of the p24 antigen assay. All specimens found positive are confirmed by neutralization for an additional charge. Quantitation of p24 antigen has been shown to be useful for detection of circulating, replicating, as well as nonviable HIV. The measurement of p24 antigenemia using the ICD modification may be of significant value for the detection of early infection and as a marker of disease progression and therapeutic response. Furthermore the p24/ICD assay has shown significant value for the early detection of HIV infection in neonates and may supplement other diagnostic tests for neonatal infections, such as HIV-1 PCR. Reactive specimens are confirmed by a neutralization method for an additional charge.​
Reference Range Information
Negative
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87390 ​ ​ ​ ​
Synonyms/Keywords
​Human Immunodeficiency Virus Type I (HIV-1) p24 Antigen Assay, p24 Antigen
Ordering Applications
Ordering Application Description
​Centricity ​HIV-1 p24 Ag Assay
​Cerner ​HIV 1 P24 Antigen
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Serum​ Red Top Tube (RTT)​ Serum Separator Tube (SST)​ 1.0 mL​ ​0.5 mL ​
Collection Processing
​Centrifuge and remove serum from cells within 24 hours of collection. Send serum frozen in a screw-capped plastic vial.
Specimen Stability Information
Specimen Type Temperature
​Serum Frozen​
Rejection Criteria
Heat-inactivated specimens
Hemolyzed samples​
Specimens containing particulate matter or precipitate ​
Specimens other than serum ​
​Cadaver specimens
Diluted specimens ​
Reference Range Information
Negative
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Focus Diagnostics​ ​ 2 to 6 days​ ELISA (Enzyme Linked Immunosorbent Assay) ​
Reference Lab
Focus Diagnostics
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87390 ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.