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23179 West Nile Confirmation

West Nile Confirmation
Test Code: MISC
Useful For
 In general, West Nile confirmation testing (WNV) should only occur as a result of a positive WNV IgM screening test (serology).
For WNV serological screen testing ordered and performed at Marshfield Laboratories (WNVAB), confirmatory testing will automatically be performed on samples that test positive for IgM antibodies. Confirmation testing will be performed by the Wisconsin State Lab of Hygiene.  Samples testing positive for IgG antibodies will NOT be routinely forwarded for confirmation. ​
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Serum​ ​Red Top Tube (RTT) ​ ​1 mL ​ ​
​CSF ​Sterile screw-top container ​ ​1 mL ​ ​
Specimen Stability Information
Specimen Type Temperature Time
​Serum or CSF ​ Refrigerate​ ​72 hours
Frozen​ Indefinite​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Wisconsin State Lab of Hygiene​ Varies​ 10-14 days​ Neutalization​
Reference Lab
Wisconsin State Lab of Hygiene
Test Information
In general, West Nile confirmation testing (WNV) should only occur as a result of a positive WNV IgM screening test (serology).
For WNV serological screen testing ordered and performed at Marshfield Laboratories (WNVAB), confirmatory testing will automatically be performed on samples that test positive for IgM antibodies. Samples testing positive for IgG antibodies will NOT be routinely forwarded for confirmation.
Confirmation testing will be performed by the Wisconsin State Lab of Hygiene. Turnaround time is 10-14 days.
WNV confirmatory testing for CSF samples and followup on serum IgG samples should be submitted as a 'Miscellaneous' test request for West Nile Confirmatory testing. ​
Reference Range Information
Performing Location Reference Range
Wisconsin State Lab of Hygiene​ No reference range available​
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Serum​ ​Red Top Tube (RTT) ​ ​1 mL ​ ​
​CSF ​Sterile screw-top container ​ ​1 mL ​ ​
Specimen Stability Information
Specimen Type Temperature Time
​Serum or CSF ​ Refrigerate​ ​72 hours
Frozen​ Indefinite​
Useful For
 In general, West Nile confirmation testing (WNV) should only occur as a result of a positive WNV IgM screening test (serology).
For WNV serological screen testing ordered and performed at Marshfield Laboratories (WNVAB), confirmatory testing will automatically be performed on samples that test positive for IgM antibodies. Confirmation testing will be performed by the Wisconsin State Lab of Hygiene.  Samples testing positive for IgG antibodies will NOT be routinely forwarded for confirmation. ​
Reference Range Information
Performing Location Reference Range
Wisconsin State Lab of Hygiene​ No reference range available​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Wisconsin State Lab of Hygiene​ Varies​ 10-14 days​ Neutalization​
Reference Lab
Wisconsin State Lab of Hygiene
For billing questions, see Contacts
For most current information refer to the Marshfield Laboratory online reference manual.