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23217 Amniotic Fluid Culture for Genetic Testing (AFC)

Amniotic Fluid Culture for Genetic Testing (AFC)
Test Code: AFGENSO
Synonyms/Keywords
​Ref Lab Code: 80334, Amniotic Fluid Culture for Genetic Testing, Cell culture, Amniotic fluid, Culture for Genetic Testing, Amniotic Fluid, Genetic Testing Culture, Amniotic Fluid, Saving cells for possible genetic testing
Useful For
Producing amniocyte cultures that can be used for genetic analysis
 
Once confluent flasks are established, the amniocyte cultures are sent to other laboratories, either within Mayo Clinic or to external sites, based on the specific testing requested.​
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Amniotic Fluid​ Amniotic fluid container​ ​ ​5-10 mL ​5 mL ​
Collection Processing Instructions
Provide a reason for referral and gestational age with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.
 
1. Discard the first 2 mL of amniotic fluid. If the culture will be performed in conjunction with chromosome analysis and alpha-fetoprotein, a total of approximately 25 mL to 30 mL will be needed for the combined studies.
2. Place the tubes in a Styrofoam container (Supply T329).
3. Fill remaining space with packing material.
4. Unavoidably, about 1% to 2% of mailed-in specimens are not viable.
5. Bloody specimens are undesirable.
6. If the specimen does not grow in culture, you will be notified within 7 days of receipt. ​
Specimen Stability Information
Specimen Type Temperature
​Amniotic fluid ​Ambient (preferred)
​ ​Refrigerated
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Medical Laboratories

Monday through Friday; 6am - 9am

Saturday, Sunday; 6am - 4pm​

 23 days​
Cell Culture​
Reference Lab
Mayo Clinic Laboratories
Test Information
Aminocyte culture for prenatal genetic testing. The additional test(s) desired must be indicated on the request form that accompanies the specimen.
Fetal cells obtained by amniocentesis (amniocytes) are used for a wide range of laboratory tests. Prior to testing, the cells may need to be cultured to obtain adequate numbers of amniocytes.
Reference Range Information
Not applicable
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
88235​ ​ ​ Tissue culture, fluid​ ​
​88240 ​ ​ ​Cryopreservation ​
Synonyms/Keywords
​Ref Lab Code: 80334, Amniotic Fluid Culture for Genetic Testing, Cell culture, Amniotic fluid, Culture for Genetic Testing, Amniotic Fluid, Genetic Testing Culture, Amniotic Fluid, Saving cells for possible genetic testing
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Amniotic Fluid​ Amniotic fluid container​ ​ ​5-10 mL ​5 mL ​
Collection Processing
Provide a reason for referral and gestational age with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.
 
1. Discard the first 2 mL of amniotic fluid. If the culture will be performed in conjunction with chromosome analysis and alpha-fetoprotein, a total of approximately 25 mL to 30 mL will be needed for the combined studies.
2. Place the tubes in a Styrofoam container (Supply T329).
3. Fill remaining space with packing material.
4. Unavoidably, about 1% to 2% of mailed-in specimens are not viable.
5. Bloody specimens are undesirable.
6. If the specimen does not grow in culture, you will be notified within 7 days of receipt. ​
Specimen Stability Information
Specimen Type Temperature
​Amniotic fluid ​Ambient (preferred)
​ ​Refrigerated
Useful For
Producing amniocyte cultures that can be used for genetic analysis
 
Once confluent flasks are established, the amniocyte cultures are sent to other laboratories, either within Mayo Clinic or to external sites, based on the specific testing requested.​
Reference Range Information
Not applicable
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Medical Laboratories

Monday through Friday; 6am - 9am

Saturday, Sunday; 6am - 4pm​

 23 days​
Cell Culture​
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
88235​ ​ ​ Tissue culture, fluid​ ​
​88240 ​ ​ ​Cryopreservation ​
For most current information refer to the Marshfield Laboratory online reference manual.