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24781 MSI by IHC and PCR (672 & 666)

MSI by IHC and PCR (672 & 666)
Test Code: MSITSO
Synonyms/Keywords
Ref Lab Code: G00672, MICROSATELLITE INSTABILITY (MSI), TUMOR (672 & 664)
Specimen Requirements
​See "Collection Processing Instructions"
Collection Processing Instructions

 This assay requires submission of normal tissue (normal colonic mucosa or normal tissue from other sites of the same patient OR patient's peripheral blood sample) for comparison with tumor.

 NORMAL CONTROL:
       1. 10-20 mL whole blood EDTA Lavender Top Tube (LTT). Refrigerated (preferred).
    OR
       2. Normal tissue submitted as a Fixed Paraffin Block and marked "Normal Tissue."
TUMOR TISSUE:
       1. Fixed Paraffin Block with corresponding H&E. Tissue should be well fixed in formalin. If an alternate fixative is used, note on order. Tissue should average 5 mm (0.2 cm x 0.2 cm x 0.2 cm tissue). Store at room temperature and ship with cold pack.
    OR
       2. Unstained slides. All slides must be sent within 6 weeks of cutting. For the PCR portion of this test: Precut 7 micron sections from paraffin block. Send 4 slides of tumor and 2-3 slides of normal tissue (if not submitting peripheral blood). Mount on uncoated slides. For the IHC portion of this test: Precut 5 micron sections from paraffin block and mount on charged Poly-L-Lysine coated slides. Minimum of 4 slides per antibody (total of 16 required), plus H&E. Air dry (do not oven dry) and store at room temperature. ​

Specimen Stability Information
Specimen Type Temperature Time
Tissue/Slides​ Room temperature​ ​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Integrated Oncology​
 Monday through Friday​ ​6-7 days IHC and PCR​
Reference Lab
Integrated Oncology
Test Information
Detects instability of microsatellite loci associated with a loss or suppression of DNA mismatch repair system. Detects instability in 5 mononucleotide markers. This is providing both the PCR testing and IHC stains for MLH1/MHS-2 and MSH-6/PMS2​
Reference Range Information
Interpretive report.
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
81301 ​ ​ ​ Microsatellite Instability gene analysis​ ​
88381​ ​ ​ Microdissection, manual​ ​
88342​ ​ ​4 IHC, each antibody​ ​
Synonyms/Keywords
Ref Lab Code: G00672, MICROSATELLITE INSTABILITY (MSI), TUMOR (672 & 664)
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
​See "Collection Processing Instructions"
Collection Processing

 This assay requires submission of normal tissue (normal colonic mucosa or normal tissue from other sites of the same patient OR patient's peripheral blood sample) for comparison with tumor.

 NORMAL CONTROL:
       1. 10-20 mL whole blood EDTA Lavender Top Tube (LTT). Refrigerated (preferred).
    OR
       2. Normal tissue submitted as a Fixed Paraffin Block and marked "Normal Tissue."
TUMOR TISSUE:
       1. Fixed Paraffin Block with corresponding H&E. Tissue should be well fixed in formalin. If an alternate fixative is used, note on order. Tissue should average 5 mm (0.2 cm x 0.2 cm x 0.2 cm tissue). Store at room temperature and ship with cold pack.
    OR
       2. Unstained slides. All slides must be sent within 6 weeks of cutting. For the PCR portion of this test: Precut 7 micron sections from paraffin block. Send 4 slides of tumor and 2-3 slides of normal tissue (if not submitting peripheral blood). Mount on uncoated slides. For the IHC portion of this test: Precut 5 micron sections from paraffin block and mount on charged Poly-L-Lysine coated slides. Minimum of 4 slides per antibody (total of 16 required), plus H&E. Air dry (do not oven dry) and store at room temperature. ​

Specimen Stability Information
Specimen Type Temperature Time
Tissue/Slides​ Room temperature​ ​
Reference Range Information
Interpretive report.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Integrated Oncology​
 Monday through Friday​ ​6-7 days IHC and PCR​
Reference Lab
Integrated Oncology
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
81301 ​ ​ ​ Microsatellite Instability gene analysis​ ​
88381​ ​ ​ Microdissection, manual​ ​
88342​ ​ ​4 IHC, each antibody​ ​
For most current information refer to the Marshfield Laboratory online reference manual.