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25047 Legionella species, PCR (LEGRP)

Legionella species, PCR (LEGRP)
Test Code: LEGPSO
Synonyms/Keywords
​Ref Lab Code: 89564, Legionella PCR
Useful For
Sensitive and rapid diagnosis of pneumonia caused by Legionella species
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Bronchial washing, bronchoalveolar lavage, lung tissue, pleural fluid, sputum, transtracheal aspirate, or tracheal secretions​ Sterile container​ ​ ​1 mL ​0.5 mL ​
Specimen Stability Information
Specimen Type Temperature Time
​Respiratory specimen ​Refrigerated ​
​Lung tissue ​Refrigerated ​< 7 days (preferred)
​ ​Frozen ​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Medical Laboratories Monday through Sunday​ 1 day​
Rapid Polymerase Chain Reaction (PCR)
(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)​
Reference Lab
Mayo Clinic Laboratories
Test Information
​A positive PCR result for the presence of a specific sequence found within the Legionella 5S rRNA gene indicates the presence of a Legionella species DNA in the specimen and supports a diagnosis of legionellosis.
A negative PCR result indicates the absence of detectable Legionella DNA in the specimen, but does not rule out legionellosis as false negative results may occur due to inhibition of PCR, sequence variability underlying the primers and/or probes or the presence of Legionella species in quantities less than the limit of detection of the assay.
This assay does not differentiate between the Legionella species. Although not specifically addressed in validation studies, the assay is not recommended as a test of cure because nucleic acid may persist after successful treatment. False positive results are theoretically possible if patient specimens are contaminated with Legionella DNA, which may occur since Legionella species are environmental organisms present in aquatic environments.
Reference Range Information
Performing Location Reference Range
​Mayo Medical Laboratories ​Negative
Interpretation
A positive PCR result for the presence of a specific sequence found within the Legionella 5S rRNA gene indicates the presence of a Legionella species DNA, which may be due to Legionella infection or environmental/water Legionella DNA in the specimen.
 
A negative PCR result indicates the absence of detectable Legionella DNA in the specimen, but does not rule-out legionellosis as false-negative results may occur due to inhibition of PCR, sequence variability underlying the primers and/or probes, or the presence of Legionella species in quantities less than the limit of detection of the assay.​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87801 ​ ​ ​ ​
Synonyms/Keywords
​Ref Lab Code: 89564, Legionella PCR
Ordering Applications
Ordering Application Description
​Centricity ​Legionella Species, PCR (LEGRP)
​Cerner ​Legionella Sps, PCR (89564)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Bronchial washing, bronchoalveolar lavage, lung tissue, pleural fluid, sputum, transtracheal aspirate, or tracheal secretions​ Sterile container​ ​ ​1 mL ​0.5 mL ​
Specimen Stability Information
Specimen Type Temperature Time
​Respiratory specimen ​Refrigerated ​
​Lung tissue ​Refrigerated ​< 7 days (preferred)
​ ​Frozen ​
Useful For
Sensitive and rapid diagnosis of pneumonia caused by Legionella species
Reference Range Information
Performing Location Reference Range
​Mayo Medical Laboratories ​Negative
Interpretation
A positive PCR result for the presence of a specific sequence found within the Legionella 5S rRNA gene indicates the presence of a Legionella species DNA, which may be due to Legionella infection or environmental/water Legionella DNA in the specimen.
 
A negative PCR result indicates the absence of detectable Legionella DNA in the specimen, but does not rule-out legionellosis as false-negative results may occur due to inhibition of PCR, sequence variability underlying the primers and/or probes, or the presence of Legionella species in quantities less than the limit of detection of the assay.​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Medical Laboratories Monday through Sunday​ 1 day​
Rapid Polymerase Chain Reaction (PCR)
(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)​
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87801 ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.