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25403 ROS1 (6q22) Rearrangement, FISH, Tissue (ROS1F)

ROS1 (6q22) Rearrangement, FISH, Tissue (ROS1F)
Test Code: FROS1SO
Synonyms/Keywords
​ROS1, Lung carcinoma, Non-small cell lung cancer, NSCLC
Useful For
​Identifying ROS1 gene rearrangements in patients with late-stage, lung adenocarcinomas that are negative for EGFR mutations and ALK rearrangements.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)​​
Tissue
​FFPE
tumor tissue block
 ​Slides 4 consecutive, unstained, 5 micron thick sections placed on positively charged slides, and 1 H&E slide ​2 consecutive, unstained, 5 micron thick sections placed on positively charged slides, and 1 H&E slide ​
​​Tumor adequacy is optimally at least 300 tumor cells for FISH testing, yet a minimum of 50 cells is required. Less than 50 cells will be cancelled. ​ ​ ​ ​ ​ ​
Collection Processing Instructions

Provide a reason for referral with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.

For blocks residing at a Non-Clinic location, please complete the Surgical Pathology Specimen Request form found in FORMS PRINTER or located here:

Surgical Pathology Specimen Request

Specimen Stability Information
Specimen Type ​​Temperature
​ Tissue ​Ambient (preferred)
Refrigerated​
Rejection Criteria
​< 50 tumor cells
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentati​​on
Mayo Medical Laboratories ​Monday through Sunday ​7 days ​FISH
Reference Lab
Mayo Clinic Laboratories
Reference Range Information
Performing Location Reference Range​​
Mayo Medical Laboratories​ ​Interpretive Report
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments​​
88291 ​ 1 Interpretation and report ​
​88271​ ​ 2 ​Probe Set, 1ST ​
​​88271 ​ 2 ​Probe, +2 ​as needed
​88271​ ​ ​1 ​Probe, +1 ​as needed
​88271​ ​ ​2 ​Probe, +2 ​as needed
​88271​ ​ ​3 ​Probe, +3 ​as needed
​88274​ ​ ​1 ​Interphases, <25​ ​as needed
​​88274 ​ ​1 ​Interphases, 25-99 ​as needed
​88275​ ​ ​1 ​Interphases, >100 ​as needed
Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.
Synonyms/Keywords
​ROS1, Lung carcinoma, Non-small cell lung cancer, NSCLC
Ordering Applications
Ordering Application Description
COM​​​​ ​ROS1 (6q22) Rearrangement
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)​​
Tissue
​FFPE
tumor tissue block
 ​Slides 4 consecutive, unstained, 5 micron thick sections placed on positively charged slides, and 1 H&E slide ​2 consecutive, unstained, 5 micron thick sections placed on positively charged slides, and 1 H&E slide ​
​​Tumor adequacy is optimally at least 300 tumor cells for FISH testing, yet a minimum of 50 cells is required. Less than 50 cells will be cancelled. ​ ​ ​ ​ ​ ​
Collection Processing

Provide a reason for referral with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.

For blocks residing at a Non-Clinic location, please complete the Surgical Pathology Specimen Request form found in FORMS PRINTER or located here:

Surgical Pathology Specimen Request

Specimen Stability Information
Specimen Type ​​Temperature
​ Tissue ​Ambient (preferred)
Refrigerated​
Rejection Criteria
​< 50 tumor cells
Useful For
​Identifying ROS1 gene rearrangements in patients with late-stage, lung adenocarcinomas that are negative for EGFR mutations and ALK rearrangements.
Reference Range Information
Performing Location Reference Range​​
Mayo Medical Laboratories​ ​Interpretive Report
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentati​​on
Mayo Medical Laboratories ​Monday through Sunday ​7 days ​FISH
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments​​
88291 ​ 1 Interpretation and report ​
​88271​ ​ 2 ​Probe Set, 1ST ​
​​88271 ​ 2 ​Probe, +2 ​as needed
​88271​ ​ ​1 ​Probe, +1 ​as needed
​88271​ ​ ​2 ​Probe, +2 ​as needed
​88271​ ​ ​3 ​Probe, +3 ​as needed
​88274​ ​ ​1 ​Interphases, <25​ ​as needed
​​88274 ​ ​1 ​Interphases, 25-99 ​as needed
​88275​ ​ ​1 ​Interphases, >100 ​as needed
Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.
For most current information refer to the Marshfield Laboratory online reference manual.