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25482 JAK2 V617F with reflex to CALR and MPL (MPNR)

JAK2 V617F with reflex to CALR and MPL (MPNR)
Test Code: MPNRSO
Synonyms/Keywords
83872-JAK2B, Janus kinase 2 gene, Tyrosine Kinase Mutation, CALR Calreticulin Essential Thrombocythemia, JAK2-negative Myeloproliferative Neoplasm, Myelofibrosis, Myeloproliferative Disorder, Myeloproliferative Neoplasm (MPN), Primary Myelofibrosis, MPL S505, MPLW515, Myeloproliferative leukemia virus oncogene
Test Components
​JAK2, V617F,(JAK2B), CALR (CALX), MPL (exon10) (MPLR)
Useful For
This reflex test sequentially evaluates for the common major gene mutations associated with non-BCR/ABL1 positive myeloproliferative neoplasms until a mutation is identified. The testing sequence is based on the reported frequency of gene mutations in this disease group. Initial testing evaluates for the presence of the JAK2 V617F mutation. If this result is negative, testing proceeds with assessment for CALR mutations. If the CALR result is also negative, then testing proceeds to evaluate for mutations in exon 10 of the MPL gene.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)​​
​Whole Blood ​Lavender Top Tube (EDTA) ​Yellow Top Tube (ACD solution B) ​3 mL ​0.5 mL ​
​Bone Marrow ​Lavender Top Tube (EDTA) ​​Yellow Top Tube (ACD solution B) ​2 mL

​0.5 mL

 ​
​Extractd DNA from blood or bone marrow ​1.5  to 2 mL tube with indication of volume and concentration of DNA ​ ​Entire specimen ​ ​
Collection Processing Instructions

The following information is required:
1. Pertinent clinical history
2. Clinical or morphologic suspicion
3. Date of collection
4. Specimen source

Specimen must arrive within 168 hours (7 days) of collection.

Send whole blood or bone marrow specimens in original tube.  Label must indicate sample type.

Specimen Stability Information
Specimen Type Temperature Ti​​me
Whole Blood​ ​ Ambient (preferred) ​7 days
​Refrigerate ​7 days
Bone Marrow ​ ​Ambient (preferred) ​7 days
​Refrigerate ​7 days
Extraced DNA from blood or bone marrow​ ​ ​Ambient (preferred) ​7 days
​Refrigerate ​7 days
Rejection Criteria
Gross hemolysis
​Paraffin embedded bone marrow aspirate clot or biopsy blocks, slides, or paraffin shavings
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrum​​entation
​Mayo Medical Laboratories ​Monday through Friday ​7 days ​Point Mutation Detection in DNA using Quantitaive Polymerase Chain Reaction (PCR)
Reference Lab
Mayo Clinic Laboratories
Reference Range Information
See report
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Commen​​ts
81270 ​ ​1 ​Jak2 V617F variant ​
81219 ​ ​1 ​CALR Gene Mutation, Exon 9 ​If needed
​81403 ​ ​1 ​MPL exon 10 sequence ​If needed
Synonyms/Keywords
83872-JAK2B, Janus kinase 2 gene, Tyrosine Kinase Mutation, CALR Calreticulin Essential Thrombocythemia, JAK2-negative Myeloproliferative Neoplasm, Myelofibrosis, Myeloproliferative Disorder, Myeloproliferative Neoplasm (MPN), Primary Myelofibrosis, MPL S505, MPLW515, Myeloproliferative leukemia virus oncogene
Test Components
​JAK2, V617F,(JAK2B), CALR (CALX), MPL (exon10) (MPLR)
Ordering Applications
Ordering Application Descri​​ption
​Clinic Order Manager ​JAK2 V617F w reflx CALR & MPL
​Centricity ​JAK2 V617F w reflx CALR & MPL
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)​​
​Whole Blood ​Lavender Top Tube (EDTA) ​Yellow Top Tube (ACD solution B) ​3 mL ​0.5 mL ​
​Bone Marrow ​Lavender Top Tube (EDTA) ​​Yellow Top Tube (ACD solution B) ​2 mL

​0.5 mL

 ​
​Extractd DNA from blood or bone marrow ​1.5  to 2 mL tube with indication of volume and concentration of DNA ​ ​Entire specimen ​ ​
Collection Processing

The following information is required:
1. Pertinent clinical history
2. Clinical or morphologic suspicion
3. Date of collection
4. Specimen source

Specimen must arrive within 168 hours (7 days) of collection.

Send whole blood or bone marrow specimens in original tube.  Label must indicate sample type.

Specimen Stability Information
Specimen Type Temperature Ti​​me
Whole Blood​ ​ Ambient (preferred) ​7 days
​Refrigerate ​7 days
Bone Marrow ​ ​Ambient (preferred) ​7 days
​Refrigerate ​7 days
Extraced DNA from blood or bone marrow​ ​ ​Ambient (preferred) ​7 days
​Refrigerate ​7 days
Rejection Criteria
Gross hemolysis
​Paraffin embedded bone marrow aspirate clot or biopsy blocks, slides, or paraffin shavings
Useful For
This reflex test sequentially evaluates for the common major gene mutations associated with non-BCR/ABL1 positive myeloproliferative neoplasms until a mutation is identified. The testing sequence is based on the reported frequency of gene mutations in this disease group. Initial testing evaluates for the presence of the JAK2 V617F mutation. If this result is negative, testing proceeds with assessment for CALR mutations. If the CALR result is also negative, then testing proceeds to evaluate for mutations in exon 10 of the MPL gene.
Test Components
​JAK2, V617F,(JAK2B), CALR (CALX), MPL (exon10) (MPLR)
Reference Range Information
See report
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrum​​entation
​Mayo Medical Laboratories ​Monday through Friday ​7 days ​Point Mutation Detection in DNA using Quantitaive Polymerase Chain Reaction (PCR)
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Commen​​ts
81270 ​ ​1 ​Jak2 V617F variant ​
81219 ​ ​1 ​CALR Gene Mutation, Exon 9 ​If needed
​81403 ​ ​1 ​MPL exon 10 sequence ​If needed
For most current information refer to the Marshfield Laboratory online reference manual.