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25534 PML-RARA Translocation, t(15;17) by RT-PCR, Quantitative (2002871)

PML-RARA Translocation, t(15;17) by RT-PCR, Quantitative (2002871)
Test Code: MISC
Synonyms/Keywords
AMPL' APL, APL MRD, APL Post-consolidation therapy testing, PML-RARA fusion types, A, B, B variant, PML-RARA long, short, variable types, PML-RARA S, L, V forms, PML_RARA fusion types, Quantitative PML, t(15;17)
Useful For

Detect and quantitate PML-RARA fusion transcripts in patients with acute promyelocytic leukemia.  

Monitor minimal residual disease and assess the risk of disease relapse. 

This assay qualifies as COG approved for the AAML0631 study.

Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ ​Whole Blood ​EDTA Lavender Top Tube (LTT) ​ ​5 mL ​1 mL ​
​No ​Bone Marrow ​EDTA Lavender Top Tube (LTT) ​ ​3 mL ​1 mL ​
​No​Extracted RNA​ARUP Tissue Transport Kit​​40 uL with at least 40 ng/uL concentration​40 uL​
Collection Processing Instructions

Call Cytogenetics at 16388 with cases associated to the AAML0631 study.

Specimens must be received with 48 hours of collection due to lability of RNA.

Whole Blood and Bone Marrow:  CRITICAL REFRIGERATED.  Separate specimens must be submitted when multiple tests are ordered. 

For Extracted RNA:  Transport 40 uL RNA with at least 40 ng/uL concentration (Min:  40 uL).  CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.     

Specimen Stability Information
Specimen Type Temperature​Time
​Whole Blood ​ ​Room Temperature​1 hour
​Refrigerated​48 hours
​Bone Marrow ​ ​Room Temperature​1 hour
​Refrigerated ​48 hours
Extracted RNA Frozen ​Indefinitely
Rejection Criteria
CSF Serum
​Plasma ​Clotted sample
​Severely hemolyzed sample ​Anticoagulant other than EDTA
​Extracted DNA​RNA extracted by a non-CLIA lab
​Bone Core​FFPE tissue
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​ARUP Labs

​RNA Isolation: Sunday through Saturday

Assay: Sunday, Tuesday, Thursday

 ​2-7 days ​Reverse Transciption Polymerase Chain Reaction (RT-PCR)
Reference Lab
ARUP Laboratories
Reference Range Information
Performing Location Reference Range
​ARUP Labs ​By report
Interpretation
​Refer to report
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​81315 ​ ​ ​ ​
Synonyms/Keywords
AMPL' APL, APL MRD, APL Post-consolidation therapy testing, PML-RARA fusion types, A, B, B variant, PML-RARA long, short, variable types, PML-RARA S, L, V forms, PML_RARA fusion types, Quantitative PML, t(15;17)
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ ​Whole Blood ​EDTA Lavender Top Tube (LTT) ​ ​5 mL ​1 mL ​
​No ​Bone Marrow ​EDTA Lavender Top Tube (LTT) ​ ​3 mL ​1 mL ​
​No​Extracted RNA​ARUP Tissue Transport Kit​​40 uL with at least 40 ng/uL concentration​40 uL​
Collection Processing

Call Cytogenetics at 16388 with cases associated to the AAML0631 study.

Specimens must be received with 48 hours of collection due to lability of RNA.

Whole Blood and Bone Marrow:  CRITICAL REFRIGERATED.  Separate specimens must be submitted when multiple tests are ordered. 

For Extracted RNA:  Transport 40 uL RNA with at least 40 ng/uL concentration (Min:  40 uL).  CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.     

Specimen Stability Information
Specimen Type Temperature​Time
​Whole Blood ​ ​Room Temperature​1 hour
​Refrigerated​48 hours
​Bone Marrow ​ ​Room Temperature​1 hour
​Refrigerated ​48 hours
Extracted RNA Frozen ​Indefinitely
Rejection Criteria
CSF Serum
​Plasma ​Clotted sample
​Severely hemolyzed sample ​Anticoagulant other than EDTA
​Extracted DNA​RNA extracted by a non-CLIA lab
​Bone Core​FFPE tissue
Useful For

Detect and quantitate PML-RARA fusion transcripts in patients with acute promyelocytic leukemia.  

Monitor minimal residual disease and assess the risk of disease relapse. 

This assay qualifies as COG approved for the AAML0631 study.

Reference Range Information
Performing Location Reference Range
​ARUP Labs ​By report
Interpretation
​Refer to report
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​ARUP Labs

​RNA Isolation: Sunday through Saturday

Assay: Sunday, Tuesday, Thursday

 ​2-7 days ​Reverse Transciption Polymerase Chain Reaction (RT-PCR)
Reference Lab
ARUP Laboratories
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​81315 ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.