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25546 BCR/ABL1, p190, mRNA Detection, Reverse Transcription-PCR (RT-PCR), Quantitative, Monitoring Assay, Varies (BA190)

BCR/ABL1, p190, mRNA Detection, Reverse Transcription-PCR (RT-PCR), Quantitative, Monitoring Assay, Varies (BA190)
Test Code: BC190SO
Synonyms/Keywords
​Philadelphia Chromosome
Useful For
Monitoring response to therapy in patients with known e1/a2 bcr/abl (p190) fusion forms.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
 Submit 1 of the following specimens:​ ​ ​ ​ ​ ​ ​
​No ​Whole Blood ​EDTA Lavender Top Tube (LTT) ​ACD Yellow Top Tube (YTT) ​10 mL 4 mL ​
​N/A ​Bone Marrow ​EDTA Lavender Top Tube (LTT) ​ACD Yellow Top Tube (YTT) ​4 mL 2 mL ​
Collection Processing Instructions

Notify Cytogenetics (1-800-222-5835, ext. 16388) when specimen is collected. 

Pertinent clinical history including if the patient has a diagnosis of chronic myelogeneous leukemia or other BCR/ABL-1 positive neoplasm.

Label specimen as whole blood or bone marrow, invert several times to mix and ship in original collection container.

Specimen Stability Information
Specimen Type Temperature Time
​Varies ​ ​Refrigerated (preferred) 5 days
​Room Temperature ​72 hours
Rejection Criteria
Gross hemolysis
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Medical Laboratories ​Monday through Friday ​4 days ​Quantitative Reverse Transcription-Polymerase Chain Reaction (RT-PCR)
Reference Lab
Mayo Clinic Laboratories
Test Information

mRNA transcribed from BCR/ABL (fusion of the breakpoint cluster region gene [BCR]at chromosome 22q11 to the Abelson gene [ABL] at chromosome 9q23) is detected in all chronic myelogenous leukemia (CML) patients and a subset of both acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML) patients. Although breakpoints in the BCR and ABL genes may occur in a variety of locations, splicing of the primary RNA transcripts result in only 8 fusion site variants (e1/a2, e6/a2, e13/a2, e14/a2, e19/a2, and e1/a3, e13/a3, e14/a3), which incorporate the entire sequence of the exons on both sides of the fusion site. The e1/a2 and e1/a3 fusion forms produce a 190-kDa protein designated p190. This bcr/abl protein form is found in approximately 75% of childhood ALL patients and approximately 50% of adult ALL patients, with the majority arising from e1/a2 mRNA. The p190 is also the predominant fusion form in a small subset of CML patients, although the vast majority of CML cases contain the p210 protein, typically from e13/a2 or e14/a2 mRNA fusions. Other fusion forms are very rare.

Quantitative reverse-transcription PCR (qRT-PCR) is the most sensitive method for monitoring bcr/abl levels during treatment. This test detects mRNA coding for the most common p190 fusion form (e1/a2).

Reference Range Information
Performing Location Reference Range
​Mayo Medical Labs ​ ​Interpretive Report ​
Interpretation
An interpretive report will be provided. The presence or absence of the BCR/ABL mRNA (bcr/abl) fusion form producing the p190 fusion protein is reported. If positive, the level is reported as the ratio of bcr/abl (p190) to abl with conversion to a percentage (ie, bcr/abl (p190) as a percentage of total abl).
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​81207 ​ ​ ​ ​
Synonyms/Keywords
​Philadelphia Chromosome
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
 Submit 1 of the following specimens:​ ​ ​ ​ ​ ​ ​
​No ​Whole Blood ​EDTA Lavender Top Tube (LTT) ​ACD Yellow Top Tube (YTT) ​10 mL 4 mL ​
​N/A ​Bone Marrow ​EDTA Lavender Top Tube (LTT) ​ACD Yellow Top Tube (YTT) ​4 mL 2 mL ​
Collection Processing

Notify Cytogenetics (1-800-222-5835, ext. 16388) when specimen is collected. 

Pertinent clinical history including if the patient has a diagnosis of chronic myelogeneous leukemia or other BCR/ABL-1 positive neoplasm.

Label specimen as whole blood or bone marrow, invert several times to mix and ship in original collection container.

Specimen Stability Information
Specimen Type Temperature Time
​Varies ​ ​Refrigerated (preferred) 5 days
​Room Temperature ​72 hours
Rejection Criteria
Gross hemolysis
Useful For
Monitoring response to therapy in patients with known e1/a2 bcr/abl (p190) fusion forms.
Reference Range Information
Performing Location Reference Range
​Mayo Medical Labs ​ ​Interpretive Report ​
Interpretation
An interpretive report will be provided. The presence or absence of the BCR/ABL mRNA (bcr/abl) fusion form producing the p190 fusion protein is reported. If positive, the level is reported as the ratio of bcr/abl (p190) to abl with conversion to a percentage (ie, bcr/abl (p190) as a percentage of total abl).
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Medical Laboratories ​Monday through Friday ​4 days ​Quantitative Reverse Transcription-Polymerase Chain Reaction (RT-PCR)
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​81207 ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.