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26120 Mycoplasma genitalium, PCR (MGRP)

Mycoplasma genitalium, PCR (MGRP)
Test Code: MGRPSO
Useful For
Detection of Mycoplasma genitalium from genitourinary and reproductive sources.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​Urogenital, Vaginal, Cervix, Urethra ​M6 Transport Media ​M4 or M5 Transport Media, Swab in Transport Media, Culturette (BBL Culture Swab), Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium) ​One swab ​One swab ​
​Fluids, Amniotic, Pelvic, Prostatic, Semen ​Sterile, screw-capped container ​M4, M5 or M6 Transport Media ​1-10 mL ​1 mL ​
​Urine ​Sterile, screw-capped container ​ ​10 mL ​2 mL ​
​Tissue, Placenta, Genitourinary ​​Sterile, screw-capped container ​ ​5 mm ​ ​
Collection Processing Instructions
​Specimens should be delivered to the laboratory as soon as possible.
 
Do not use calcium alginate or wooden shaft swabs.
 
Samples should be taken without contact with antiseptics or lubricants.
 
For more information on Specimen Collection Media/Swab, see Specimen Transport Pictorial.
Specimen Stability Information
Specimen Type Temperature Time
​All specimens ​ ​Refrigerate (preferred) ​7 days
​Frozen ​7 days
Rejection Criteria
Cotton or calcium alginate-tipped swab, wooden shaft swab, dry swabs, swab containing gel or charcoal
​Formalin-fixed and/or paraffin-embedded tissues
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Medical Laboratories ​Monday through Friday ​3 days ​Real-Time Polymerase Chain Reaction (PCR) Using LightCycler and Fluorescent Resonance Energy Transfer (FRET)
Reference Lab
Mayo Clinic Laboratories
Test Information
Mycoplasma genitalium causes acute and chronic nongonococcal urethritis, cervicitis, and pelvic inflammatory disease. Culture isolation is technically challenging; PCR is the diagnostic test of choice.
Reference Range Information
Performing Location Reference Range
​Mayo Medical Laboratores ​Negative
Interpretation

A positive PCR result for the presence of a specific sequence found within the Mycoplasma genitalium tuf gene indicates the presence of M genitalium DNA in the specimen.

A negative PCR result indicates the absence of detectable M genitalium DNA in the specimen, but does not rule-out infection as false-negative results may occur due to the following; inhibition of PCR, sequence variability underlying the primers or probes, or the presence of M genitalium in quantities below the limit of detection of the assay.

Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87563 ​ ​1 ​ ​
Ordering Applications
Ordering Application Description
​Centricity ​Mycoplasma genitalium, PCR (MGRP)
​Cerner ​Mycoplasma genitalium, PCR (MGRP)
​COM ​Mycoplasma genitalium, PCR (MGRP)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​Urogenital, Vaginal, Cervix, Urethra ​M6 Transport Media ​M4 or M5 Transport Media, Swab in Transport Media, Culturette (BBL Culture Swab), Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium) ​One swab ​One swab ​
​Fluids, Amniotic, Pelvic, Prostatic, Semen ​Sterile, screw-capped container ​M4, M5 or M6 Transport Media ​1-10 mL ​1 mL ​
​Urine ​Sterile, screw-capped container ​ ​10 mL ​2 mL ​
​Tissue, Placenta, Genitourinary ​​Sterile, screw-capped container ​ ​5 mm ​ ​
Collection Processing
​Specimens should be delivered to the laboratory as soon as possible.
 
Do not use calcium alginate or wooden shaft swabs.
 
Samples should be taken without contact with antiseptics or lubricants.
 
For more information on Specimen Collection Media/Swab, see Specimen Transport Pictorial.
Specimen Stability Information
Specimen Type Temperature Time
​All specimens ​ ​Refrigerate (preferred) ​7 days
​Frozen ​7 days
Rejection Criteria
Cotton or calcium alginate-tipped swab, wooden shaft swab, dry swabs, swab containing gel or charcoal
​Formalin-fixed and/or paraffin-embedded tissues
Useful For
Detection of Mycoplasma genitalium from genitourinary and reproductive sources.
Reference Range Information
Performing Location Reference Range
​Mayo Medical Laboratores ​Negative
Interpretation

A positive PCR result for the presence of a specific sequence found within the Mycoplasma genitalium tuf gene indicates the presence of M genitalium DNA in the specimen.

A negative PCR result indicates the absence of detectable M genitalium DNA in the specimen, but does not rule-out infection as false-negative results may occur due to the following; inhibition of PCR, sequence variability underlying the primers or probes, or the presence of M genitalium in quantities below the limit of detection of the assay.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Medical Laboratories ​Monday through Friday ​3 days ​Real-Time Polymerase Chain Reaction (PCR) Using LightCycler and Fluorescent Resonance Energy Transfer (FRET)
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87563 ​ ​1 ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.