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26169 Mycoplasma pneu by PCR (MPRP)

Mycoplasma pneu by PCR (MPRP)
Test Code: MPRPSO
Synonyms/Keywords

​Mycoplasma pneumoniae PCR

Useful For

​Diagnosis of infections due to Mycoplasma pneumoniae

Specimen Requirements
Submit only 1 of the following specimens:​ ​ ​ ​​​​
Fasting RequiredSpecimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)		Pediatric Minimum Volume
(no repeat)
No​​Respiratory (Bronchial washing, bronchoalveolar lavage, tracheal secretions, sputum)​Sterile Container​M4, M4-RT, M5, M6 or UTM​1 mL​0.5 mL​
​​Swab (Throat, nasal or nasopharyngeal)​Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)​Culture transport swab (Stuart's media) or place swab in M4, M4-RT, M5, M6, UTM or ESwab​1 Swab​1 Swab​
​​Fluid (Pleural, pericardial, cerebrospinal)​Sterile vial​​0.5 mL​0.5 mL​
Collection Processing Instructions

​Specimen source is required.

Submit only one of the above types of specimens.

Specimen Stability Information
Specimen TypeTemperatureTime
​Varies​Refrigerated (preferred)​7 days
​​Frozen​7 days
Rejection Criteria
Swab/OtherCotton or calcium alginate-tipped swab, wooden shaft swab, transport swab containing gel or charcoal, Port-a-Cul tube, anaerobic fluid vials, or dry swab (no pledget or sponge)
Interference

​This assay should only be used for testing of respiratory tract specimens (throat swabs, nasopharyngeal swabs, tracheal secretions, sputum, and bronchoalvelor lavage fluid) and pleural/chest fluid, pericardial fluid and cerebrospinal fluid. 

Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation
​Mayo Clinic Laboratories​Monday through Sunday​3 days​Rapid Polymerase Chain Reaction (PCR) Using Light Cycler and Fluorescent Resonance Energy Transfer (FRET)
Reference Lab
Mayo Clinic Laboratories
Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories​Not applicable
Interpretation

​ A positive result indicates the presence of Mycoplasma pneumoniae

A negative result does not rule out the presence of M pneumoniae and may be due to the presence of inhibitors within the specimen matrix, or the presence of organisms at numbers below of detection of the assay. 

Outreach CPTs
CPTModifier
(if needed)		QuantityDescriptionComments
​87581​​​​
Synonyms/Keywords

​Mycoplasma pneumoniae PCR

Ordering Applications
Ordering ApplicationDescription
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Submit only 1 of the following specimens:​ ​ ​ ​​​​
Fasting RequiredSpecimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)		Pediatric Minimum Volume
(no repeat)
No​​Respiratory (Bronchial washing, bronchoalveolar lavage, tracheal secretions, sputum)​Sterile Container​M4, M4-RT, M5, M6 or UTM​1 mL​0.5 mL​
​​Swab (Throat, nasal or nasopharyngeal)​Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)​Culture transport swab (Stuart's media) or place swab in M4, M4-RT, M5, M6, UTM or ESwab​1 Swab​1 Swab​
​​Fluid (Pleural, pericardial, cerebrospinal)​Sterile vial​​0.5 mL​0.5 mL​
Collection Processing

​Specimen source is required.

Submit only one of the above types of specimens.

Specimen Stability Information
Specimen TypeTemperatureTime
​Varies​Refrigerated (preferred)​7 days
​​Frozen​7 days
Rejection Criteria
Swab/OtherCotton or calcium alginate-tipped swab, wooden shaft swab, transport swab containing gel or charcoal, Port-a-Cul tube, anaerobic fluid vials, or dry swab (no pledget or sponge)
Interference

​This assay should only be used for testing of respiratory tract specimens (throat swabs, nasopharyngeal swabs, tracheal secretions, sputum, and bronchoalvelor lavage fluid) and pleural/chest fluid, pericardial fluid and cerebrospinal fluid. 

Useful For

​Diagnosis of infections due to Mycoplasma pneumoniae

Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories​Not applicable
Interpretation

​ A positive result indicates the presence of Mycoplasma pneumoniae

A negative result does not rule out the presence of M pneumoniae and may be due to the presence of inhibitors within the specimen matrix, or the presence of organisms at numbers below of detection of the assay. 

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation
​Mayo Clinic Laboratories​Monday through Sunday​3 days​Rapid Polymerase Chain Reaction (PCR) Using Light Cycler and Fluorescent Resonance Energy Transfer (FRET)
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)		QuantityDescriptionComments
​87581​​​​
For most current information refer to the Marshfield Laboratory online reference manual.